Agarose gel plate storage method

The technology of agarose gel and preservation method is applied in the field of medicine and health, and can solve the problems of agarose-prone bacteria, troublesome, short storage time, etc.

Inactive Publication Date: 2015-05-06
SHANGHAI DIAN CLINICAL TESTING CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although great attention has been paid to the disinfection steps when preparing the gel plates, because the agarose is prone to bacteria growth, the time for

Method used

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  • Agarose gel plate storage method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0050] Example 1 Experimental chart on the day after being made ( image 3 )

[0051] Take a fresh serum sample, use the antiseptic agarose gel plate on the day of preparation for protein electrophoresis, and analyze the results:

[0052] Swim lane ALB α1 globulin alpha 2 globulin beta globulin gamma globulin 164.53.68.712.310.8 264.83.89.211.710.4 365.24.08.712.110.0 465.93.98.911.59.8 Mean (x)65.13.838.911.910.3 Standard deviation(s)0.520.150.200.320.38 Coefficient of variation (CV%) 0.813.872.312.663.75

Example Embodiment

[0053] [0053] Example 2 Experimental diagram of samples stored in a refrigerator at 4°C for 3 months ( Figure 4 )

[0054] Take a sample, and seal a preservative agarose gel plate with moisture, wet appearance, sufficient moisture, fullness, and no bacterial growth in the sealed box. Take a fresh serum sample, do a protein electrophoresis experiment with the sample taken out, and analyze the results (due to the tailing in lane 4, the results are eliminated):

[0055] Swim lane ALB α1 globulin alpha 2 globulin beta globulin gamma globulin 154.74.910.214.316.0 254.24.39.614.617.3 353.44.810.313.218.2 552.24.310.215.118.2 654.74.010.512.618.2 755.64.79.913.616.2 Mean (x)54.14.510.113.917.4 Standard deviation(s)1.190.350.320.931.03 Coefficient of variation (CV%) 2.207.833.156.725.94

Example Embodiment

[0056] Example 3 Experimental diagram of samples stored in a refrigerator at 4°C for 4 months ( Figure 5 )

[0057] Take a sample, and seal a preservative agarose gel plate with moisture, wet appearance, sufficient moisture, fullness, and no bacterial growth in the sealed box. Take a fresh serum sample, do a protein electrophoresis experiment with the taken sample, and analyze the results:

[0058] Swim lane ALB α1 globulin alpha 2 globulin beta globulin gamma globulin 161.22.87.912.415.7 263.02.58.110.116.2 360.22.87.113.016.8 460.72.67.111.717.9 560.02.67.211.818.5 662.42.86.311.616.9 762.12.77.211.416.6 862.33.06.511.516.8 961.72.78.211.915.6

[0059] 1061.02.78.411.116.7 Mean (x)16.462.727.411.6516.77 Standard deviation(s)1.000.140.720.760.89 Coefficient of variation (CV%) 1.635.149.726.575.32

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Abstract

The present invention provides an agarose gel plate storage method, wherein a sealed wrapping material is wrapped outside the agarose gel plate, and the method comprises film covering, single packaging, boxing and bagging. Compared with the method in the prior art, the method of the present invention has the following characteristics that: in order to ensure the long-term effectiveness of the kit, a purpose of the present invention is to provide a method for long-term storage of the agarose gel plate, the agarose gel prepared by the method has characteristics of no bacterial growth, strong antiseptic performance and easy storage, and the experiment results show that the storage time of the agarose gel plate stored by the agarose gel plate storage method in the 4 DEG C refrigerator is up to 6 months, such that the problem of the too short agarose gel plate storage time is solved, and the great market value is provided.

Description

technical field [0001] The invention relates to the technical field of medicine and health, in particular to a method for preserving an agarose gel plate. Background technique [0002] Electrophoresis refers to the charged test substance (protein, nucleotide, etc.) in an inert support medium (such as starch gel, cellulose acetate, agarose gel, polyacrylamide gel, etc.), under the action of an electric field , swimming towards the corresponding electrode direction according to their respective speeds, so that the components are separated into narrow zones, and the method of recording the electrophoretic zone map or calculating its content (%) with a suitable detection method. The pH of the electrophoresis buffer is between 6 and 9, and the ionic strength is 0.02 to 0.05 as the optimum. 1% agarose is commonly used as the electrophoresis support. Although the resolution of agarose gel is lower than that of polyacrylamide gel, it is easy to prepare and has a wide range of sepa...

Claims

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Application Information

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IPC IPC(8): G01N27/447
Inventor 于嘉屏陆桂琴李艳梅王月婷
Owner SHANGHAI DIAN CLINICAL TESTING CENT
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