Pancreatic cancer-associated serum/plasma tsRNA marker and probe and application thereof

A marker, a technique for pancreatic cancer, applied in the field of molecular biology

Active Publication Date: 2020-10-27
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Recent studies have found that tsRNAs can also exist stably and in abundance in serum. However, the related research on tsRNA and pancreatic cancer has not been reported so far. If abnormally expres

Method used

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  • Pancreatic cancer-associated serum/plasma tsRNA marker and probe and application thereof
  • Pancreatic cancer-associated serum/plasma tsRNA marker and probe and application thereof
  • Pancreatic cancer-associated serum/plasma tsRNA marker and probe and application thereof

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Experimental program
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Embodiment

[0030] A serum / plasma tsRNA marker associated with pancreatic cancer, wherein the serum / plasma tsRNA marker is any one or a combination of the following sequences:

[0031] tRF-Pro-AGG-004: SEQ ID No. 1;

[0032] tRF-Leu-CAG-002: SEQ ID No. 2.

[0033] A probe capable of specifically capturing the above-mentioned serum / plasma tsRNA markers related to pancreatic cancer. The probe is a custom-synthesized TaqMan tsRNA probe from Applied Biosystems.

[0034] Application of the above-mentioned serum / plasma tsRNA markers and probes in the preparation of a pancreatic cancer diagnostic kit.

[0035] A diagnostic kit for pancreatic cancer, the kit is used to detect the expression level of one or more of the above-mentioned tsRNA markers in serum / plasma.

[0036] The above-mentioned probes are included in the kit. The kit also includes commonly used reagents and enzymes for PCR reactions. The kit includes dNTP / AMV reverse transcriptase, buffer, MgCl2, DEPC water, Taq enzyme and the...

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Abstract

The invention belongs to the field of molecular biology, and discloses a pancreatic cancer-associated serum/plasma tsRNA marker and application thereof. The marker is tRF-Pro-AGG-004 and/or tRF-Leu-CAG-002. The tsRNA screened by the invention has significantly higher specificity and sensitivity for detection of pancreatic cancer patients than protein markers currently used in clinical practice, and greatly improves the accuracy of diagnosis. The marker can be used to prepare a diagnostic kit for auxiliary early diagnosis of pancreatic cancer.

Description

[0001] field of invention [0002] The invention belongs to the field of molecular biology, and relates to a serum / plasma tsRNA marker, probe and application thereof related to pancreatic cancer. Background technique [0003] Pancreatic cancer, commonly known as the "king of cancers", is one of the most malignant types of gastrointestinal tumors, and its mortality rate ranks third in the world. According to statistics, in 2018, there were 459,000 new cases of pancreatic cancer worldwide, and 432,000 deaths. Pancreatic cancer has a poor prognosis, with a 5-year survival rate of only about 5%, and a median survival period of less than 6 months. It poses a serious threat to human health and is a major problem that needs to be solved urgently. [0004] Early diagnosis of pancreatic cancer is difficult. At present, CT, endoscopic ultrasonography, MRI / magnetic resonance cholangiopancreatography (MRCP) are commonly used diagnostic methods for pancreatic cancer, but the vast majorit...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/686C12N15/113
CPCC12Q1/6886C12Q1/686C12Q2600/158C12Q2600/178C12Q2561/113C12Q2563/107C12Q2545/114C12Q2521/107
Inventor 王延博金芳芳
Owner NANJING UNIV
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