98 results about "Hydrothorax" patented technology

The invention discloses a probe and a sequence combination for simultaneous detection of various mutation types and particularly provides a kit, a method, a gene chip, a probe and a sequence combination which are high in sensitivity and specificity and used for simultaneous detection of point mutation, short segment insertion and deletion, copy number variation and fusion genes of 57 tumor driver genes listed in a detection list. The invention further discloses a sequence combination and probe associated gene chip and kit and a method for simultaneous detection of various mutation types. By detection of body fluid including blood, hydrothorax, abdominal dropsy and the like and tumor frozen tissues or paraffin sections, comprehensive tumor driver gene mutation information can be acquired, sensitivity and accuracy in tumor gene detection are improved, and accordingly clinicians can be assisted in making of individualized medication schemes to achieve best accurate treatment effects.

An anticancer high-molecular precursor medicine C-Spacer-P, where P is high- molecular pectin, C is an anticancer medicine containing amino radical or hydroxy radical, and Spacer is the spacing radical, is prepared by bonding the amino or hydroxy of an anticancer medicine with the hydroxy, carboxy, or hydroxymethyl of high- molecular pectin via spacing radical. It is an injection suitable for the solid cancer, and cancerous ascites or hydrothorax.

The invention discloses a new traditional Chinese medicine composition for treating liver ascites symptoms and a preparation method thereof. The traditional Chinese medicine composition is mainly prepared from the following medicine components: cassia twig, tuckahoe, rhizoma alismatis, Plantain seed, fiveleaf akebia, Lobelia, mulberry bark, climbing fern spore, broom cypress fruit, lysimachia, talcum, notopterygium root, ledebouriella root, acanthopanax bark, orientvine, cinnamon, clove, pangolin, dried orange peer, immature bitter orange, nut grass, rhubarb, hemlock parsley, magnolia bark, Chinese yam, white atractylodes rhizome, semen raphani, japanese sweetflag, bush-cherry seed, angelica glabra, Evodia rutaecarpa, galangal, allium macrostemon, Semen Euryales, pyrrosia lingua, ricepaperplant and oriental wormwood. The traditional Chinese medicine composition can be prepared into any commonly-used oral preparation by using the conventional traditional Chinese medicine preparation method. The invention can obviously improve the symptoms of abdominal distention, ascites, difficulty in walking, and probable obvious diaphragm elevation with expiratory dyspnea and exomphalocele before the ocurrence of ascites. Some patients possibly have the symptoms of hydrothorax, increased portal venous pressure, increased hydrostatic pressure abdominal visceral vessel beds, hypoalbuminemia diseases, excessive lymphogenesis, secondary hyperaldosteronism, reduction of renal blood flow, sodium output and urinary output and the like. The traditional Chinese medicine composition has exact and obvious clinical curative effect and high efficiency. By basically combining edible and pharmaceutical products specified in the national pharmacopoeia, the traditional Chinese medicine composition has the advantages of low cost, no toxic or side effects and the like.

The invention belongs to the medical teaching equipment technical field and relates to a fully-automatic multi-functional integrated puncture training computer dummy. The fully-automatic multi-functional integrated puncture training computer dummy comprises a high-simulation human body model, a position-automatic changing experimental table, puncture cavities, modules, arteriovenous veins, a liquid injection automatic control device, a fully-automatic arterial pulse simulator and a microcomputer controller. The dummy is characterized in that the high-simulation human body model is installed on the position-automatic changing position experimental table and is provided with various puncture cavities / modules such as pneumothorax, hydrothorax and abdominocentesis modules which are all connected with the microcomputer controller and / or a micro water pump / air pump; double-layer metal mesh electrodes are distributed on the neck, thoracic puncture and lumbar puncture positions of the dummy; the dummy is further provided with kidney, bladder, lymph gland and bone marrow puncture cavities / modules as well as a variety of puncturable central veins and peripheral veins; and arteries are connected with a fully-automatic blood circulation simulator. With the dummy adopted, dozens of puncture items can be provided; puncture position change, liquid injection, gas injection and artery pulsing are completely automated; and sound-light alarms can be given out automatically; life-like simulation effects can be realized; and teaching effects can be significantly improved.

The invention relates to a method for extracting cfDNA (cell-free deoxyribonucleic acid) from a hydrothorax sample to construct a high-throughput sequencing library, and belongs to the technical field of gene detection. The invention successfully extracts the cfDNA from hydrothorax for the first time; and after verification, the cfDNA can be used as a sample source for constructing a high-throughput sequencing library and can become an effective supplement to plasma cfDNA. Besides, large and small segments of the cfDNA in the hydrothorax sample are separated according to the method, so that the cfDNA in the hydrothorax sample is higher in purity, thereby being more suitable for DNA library construction, being more beneficial to later period mutation detection and being capable of solving the problems involving sample singleness and detection rate in conventional simplex plasma based cfDNA extraction and library construction.

The invention provides a separation method of breast cancer stem cells and belongs to the field of cell separation. The separation method comprises the following steps of: (1) preparing a hydrothorax single-cell suspension; (2) carrying out immunomagnetic bead negative sorting, and separating and removing non-breast-cancer cells, so as to obtain breast cancer epithelial cell sap; and (3) carrying out enrichment and purification on the breast cancer epithelial cell sap obtained in the step (2) by utilizing a non-adhesive culture method, so as to obtain breast cancer stem cell spheroids. Compared with the prior art, the separation method has the beneficial effects that the surfaces of the cells obtained by utilizing the separation method are not interfered by antibodies and magnetic beads; the separation efficiency of the breast cancer cells and the purity of the breast cancer cells after the breast cancer stem cells are enriched can be guaranteed; and not only can a small quantity of breast cancer epithelial cells be effectively separated, but also the separation time is shortened, and the production cost is lowered, so that the separation method is safe and effective.

The invention is a method of culturing TIL (tumour infiltration lymphocytes) by using self-pleural effusion supernatant. It centrifuges the collected malignant pleural effusion to store the supernatant as culture medium, places albugineous-layer cells in bottom-layer cells in the culture medium for culturing, so as to obtain suspended TIL, cultures the suspended TIL in a hatching box for 1 days, then adds in OKT3, IL-2, PHA, etc, and after 1 or 2 days, adds in IL-2 for culturing. The invention avoids the defects of existing methods: the infection of hepatitis B, AIDS, etc caused by using human AB serum, the pollution probability increased by overlong culturing time in vitro, increasing the burden of patients, prolonging curing time and increasing cost, etc.

The invention provides a traditional Chinese medicine composition for curing tuberculous exadative pleurisy. The bulk drugs of the traditional Chinese medicine composition include flos farfarae, root bark of white mulberry, Chinese yam, euphorbia kansui, rhizoma panacis majoris, folium eriobotryae, radix stemonae, Chinese-date, inula flower, Chinese mahonia stems, rhizoma zingiberis, rhizoma sparganii, flowers carthami, folia perillae acutae, allium macrostemon, selaginella tamariscina, vine of multiflower knotweed, exocarpium, changium smyrnioides, poria cocos, semen coicis, turmeric, schisandra chinensis, semen lepidii, radix polygonati officinalis, rhizoma anemarrhenae, dayflower, polygonum cuspidatum, adenophora tetraphylla and kaladana. The traditional Chinese medicine composition reduces untoward effect of hydrothorax absorption, shortens course of disease, reduces pleural adhesions, pleural thickening and chest pain, enables hydrothorax absorption of patients to be quick, remarkably alleviates chest pain, and greatly reduces rate of pachynsis pleurae or pleural adhesion, thereby overcoming side effects caused by western drug therapy and having a certain curative effect on curing tuberculous exadative pleurisy.

The invention relates to a MCP-2 / CCL8 protein used as a marker for identifying and diagnosing tubercular hydrothorax, and applications of the protein. The invention provides applications of the MCP-2 / CCL8 protein in preparation of diagnostic reagents or kits for identifying and diagnosing tubercular hydrothorax. The invention also provides a corresponding detection kit. The invention also provides a method for detecting or diagnosing tubercular hydrothorax.

The present invention relates to a pharmaceutical composition comprising an insulin sensitizer and a diuretic which can prevent or treat side effects such as edema, cardiac enlargement, body fluid retension or hydrothorax caused by administration of an insulin sensitizer.

The invention discloses an immunological detection method and a kit for detecting mycobacterium tuberculosis. According to the immunological detection method, mycobacterium tuberculosis specific antigens are adopted. The mycobacterium tuberculosis specific antigens comprise one or two components selected from protein 38kDa, protein CFP10, carbohydrate antigen LAM, and carbohydrate antigen TBGL. According to the invention, a multiple antigen composition is adopted as a mixed antigen for detecting tuberculosis antibodies. The method and the kit have advantages of high sensitivity, high specificity, and low false positive rate. During a real operation process, a multiple point combined determination result is improved into a single point determination result. Therefore the operation is more convenient. With the method and the kit, antibodies in tuberculosis patient body fluid samples such as serum and hydrothorax can be detected specifically in half a day. The kit can be produced in large scale with low cost. Therefore, the invention has an important significance in the controlling of tuberculosis.

The invention provides a traditional Chinese medicine plaster for treating malignant tumors, which is prepared from the following raw materials by weight portion: 80-130 portions of euphorbia pekinensis, 80-130 portions of euphorbia kansui, 80-130 portions of lilac daphne flower bud, 80-130 portions of rhizoma sparganii, 80-130 portions of rhizoma zedoariae, 60-100 portions of frankincense, 60-100 portions of golden cypress, 60-100 portions of rhizoma coptidis, 60-100 portions of rhubarb, 60-100 portions of myrrh, 40-70 portions of nux vomica, 40-70 portions of Chinese gall, 40-70 portions of pillis ophidiae, 40-70 portions of angelica, 40-70 portions of liquorice, 40-70 portions of dried rhizome of rehmannia, 40-70 portions of radix scrophulariae, 40-70 portions of radices trichosanthis, 40-70 portions of fiveleaf akebia fruit, 35-55 portions of centipede, 35-55 portions of scorpio, 35-55 portions of pseudo-ginseng and 35-55 portions of senso. The invention also provides a preparation method of the traditional Chinese medicine plaster. The traditional Chinese medicine plaster is used for treating various malignant tumors, is a preferred plaster for patients with end-stage tumors, i.e. hydrothorax, ascites, jaundice, and the like and has the advantages of innocuity, high safety, low cost, convenient preparation, and the like.

The invention relates to the field of biotechnology, in particular to an organoid culture medium and an organoid culture method. The organoid culture medium comprises a base medium, hydrothorax supernatant, enzyme hydrolyzed casein, hydrocortisone, l-glutamic acid, insulin, transferrin, cholera viruses, Y-27632, EGF, amphotericin B, penicillin and streptomycin. The organoid culture method includesperforming two-dimensional culture on tumor cells, and performing three-dimensional culture on the cultured cells to form organoid tissue. The medium formula can promote the rapid proliferation of tumor tissue while avoiding hypoxia injuries, and the culture method can greatly shorten the time required for a subsequent drug sensitivity test; the accuracy of the drug sensitivity test is improved,and the test cost is reduced.

A natural pharmaceutical preparation for treating hypoproteinemia, includes at least one member selected from the group consisting of a drug of increasing human serum albium, a drug of improving and enhance immunity, a drug of treating hepatitis B, a drug of treating Hepatitis C, a drug of treating acquired immune deficiency syndrome (AIDS), a drug of treating Tuberculosis, a drug of treat Fibrosis, a drug of treating hydrothorax, a drug of easing pain, a drug of stopping diarrhea, a drug of arresting coughing, a drug of reducing inflammation, and a drug of increasing urine.

The invention belongs to the filed of microorganism animal cell lines and provides a Chinese lung adenocarcinoma cell line with high metastases potentiality of bone, lever and adrenal gland. According to the invention, hydrothorax of an adenocarcinoma first-diagnosis patient is used as primary culture of cells; the human adenocarcinoma cell has the preservation number of CGMCC No.3137 and classified name as human lung adenocarcinoma cell line CPA-Yang3, can grow adhered to the wall and has the tumorigenesis rate up to 100 percent; the human lung adenocarcinoma cell can grow quickly and metabolize vigorously; the expression levels of cancer genes EMS1, VEGF-C, IL-6, IL-8, SVIL and AR gene are higher than the expression level of SPC-A-1 cell; and the lung adenocarcinoma cancel cell has the biological characteristics of mainly transferring the bone and having high transfer potential of the lever and the adrenal gland, high growth speed of bone transfer cells and complete cell morphology. The invention can be used for providing reference data to early diagnosis for transferring the human lung adenocarcinoma bone, further establishing the relevant gene chip technology and assessing and exploring the curative effects of various medicaments and the like by comprehensively applying the gene chip, quantifying PCR (Polymerase Chain Reaction), western blot in real time and other technologies.

The invention provides a hydrothorax and ascite malignant cell embedding method. The method comprises the following steps: putting hydrothorax and ascite in a plurality of 50-ml centrifugal tubes respectively, adding analytically pure ethyl alcohol into the centrifugal tubes respectively, and uniformly mixing and standing for 2-4h to obtain sediments; sucking up moisture in the sediment, wrapping a specimen by using a fixing mold and putting into a material drawing box; dehydrating the obtained specimen with the ethyl alcohol, and transparentizing the dehydrated specimen by adopting a transparent agent-xylene; putting the transparentized specimen in a 70-DEG C constant-temperature box for wax dipping, wherein an embedding agent is paraffin wax; and embedding the specimen after wax dipping with paraffin in an embedding workstation, pouring the specimen and the paraffin into an embedding box, putting the embedding box into cold water to rapidly solidify the paraffin after the paraffin on the surface of the embedding box is solidified, taking the paraffin block out of the embedding box and storing. The method can greatly increase the positive rate of cast-off cells, and can be used in further diagnosis of molecules, genes, immunohistochemistry and the like.

The invention discloses a kit for detection or assisted detection of tuberculous pleurisy. The kit comprises a device for detecting the expression amount of an immune factor, wherein the immune factor comprises (1) IL-6, IL-13, G-CSF, CCL1, CCL21, IP-10 (CXCL10), CCL8 (MCP-2), CXCL9 (MIG) and CXCL12 (SDF-1); or (2) at least one of IL-6, IL-13, G-CSF, CCL1, CCL21, IP-10 (CXCL10), CCL8 (MCP-2), CXCL9 (MIG) and CXCL12 (SDF-1). Experiments prove that a method for specifically identifying tuberculous pleurisy by taking hydrothorax as a detection object, utilizing a group of immunologic molecular marker, and adopting a multiple-factor microsphere detection technique is developed, and the purposes of improving the disease diagnosis accuracy rate, scientifically judging the state of illness and the like are realized by a multiple-factor combination manner.

The invention provides a set of primer for noninvasively detecting EML4-ALK fusion gene based on vesicles. The invention also provides a probe and a primer for detecting the EML4-ALK fusion gene through qPCR or digital PCR; the sequence is shown as SEQ ID NO:1-5. The primer and the probe are used for building a noninvasive, fast and high-sensitivity EML4-ALK fusion gene mutation detection method based on vesicles. The detection method has the advantages that by aiming at body fluid specimens (including blood, hydrothorax, ascitic fluid, cerebrospinal fluid and the like), clinical specimens canbe easily obtained. The detection sensitivity of the method is high; EML4-ALK mutation containing 10 copies can be stably detected; the precise medicine use guide can be provided.

The invention relates to mycobacterium tuberculosis specific fusion protein as well as preparation and an application of the mycobacterium tuberculosis specific fusion protein and belongs to the technical field of medical immunology diagnosis of tuberculosis. The fusion protein is formed by sequentially connecting antigenic epitopes of three kinds of protein: 38kDa, Rv0577 and Rv1271c, wherein the nucleotide sequence of the antigenic epitope of the protein 38kDa is shown as a sequence 1 in a sequence table, the nucleotide sequence of the antigenic epitope of the protein Rv0577 is shown as a sequence 2 in the sequence table, and the nucleotide sequence of the antigenic epitope of the protein Rv1271c is shown as a sequence 3 in the sequence table. Compared with the commercial antibody detection reagent box in the prior part, the prepared mycobacterium tuberculosis specific fusion protein provided by the invention has the advantages that in the tuberculosis serology diagnosis aspect, the sensitivity is high, the specificity is high, the complementarity with other antigens is realized, and the mycobacterium tuberculosis specific fusion protein can be used for detecting specific antitubercle antibodies in body fluid specimens of serum, hydrothorax and the like.

The invention discloses a method for separating lung cancer cell from hydrothorax. The method comprises the following steps of: (1) preparing Percoll solutions with different concentrations; (2) preparing hydrothorax suspension; (3) preparing Percoll layers with discontinuous density gradients; (4) centrifuging; (5) sampling; and (6) performing cell culture. The method disclosed by the invention is simple and stable and has the advantages of low cost and good effect.

The invention discloses a semi-automatic method for producing pleural and ascites liquid-based cytology. After the sample is collected, it is placed in a preservation solution bottle, and the preservation solution bottle is placed on a vibrator, and then passed through a centrifuge tube with a sharp bottom in a centrifuge. Add the cell dispersion liquid into the sampler, absorb and discard the supernatant in the test tube, remove the remaining liquid, and place the centrifuged sample on the sample delivery device of the film maker, and each sample passes through the spectrometer in turn, and the sample is aspirated The sample is placed on the corresponding slide in the transfer module, and the dried slide can be used for Papanicolaou staining or HE staining for cytological diagnosis, and Feulgen special staining for DNA quantitative analysis. The present invention is mainly used to improve and solve the problems existing in the non-cervical cell extraction, including the small number of cells, the overlapping of cells, the long production time, the background impurities of the produced films, and the low degree of automation. The quality of slides is improved, the number and distribution of cells on slides are more uniform, easy to use, and has the value of popularization and application.

A rapid stereoscopic positioning fixing system relates to human tissue body surface positioning, and is widely applied to operation procedure navigation and positioning, such as brain illness, cerebral hemorrhage puncture positioning, brain tumor surgical positioning, hydrothorax puncture positioning, renal biopsy, liver biopsy, and extraction of in-vivo foreign matters. The rapid stereoscopic positioning fixing system comprises a set of spherical membrane sticking combination, wherein the spherical membrane sticking combination comprises at least one spherical sticking membrane that is a membrane; an adhesive layer is arranged on the back side of the spherical sticking membrane; a coordinate is arranged on the spherical sticking membrane, and comprises line segments arranged according to arithmetic progression; and the line segments are developing line segments that are parallel to one another. The rapid stereoscopic positioning fixing system is rapid and time-saving in positioning, simple, convenient and labor-saving, accurate and reliable, strong in practicability, convenient to learn and popularize, and easy for wide application.

The invention relates to an external traditional Chinese medicine composition for treating hydrothorax and ascites. The external traditional Chinese medicine composition is prepared from, by weight, 20-30 parts of semen lepidii, 20-30 parts of semen pharbitidis, 5-10 parts of radix Kansui, 10-15 parts of cortex cinnamomi, 15-25 parts of radix notoginseng and 20-30 parts of cassia twigs. The external traditional Chinese medicine composition has the advantage that mass clinical observation proves that the external traditional Chinese medicine composition has a significant treatment effect on hydrothorax and ascites. The pure traditional Chinese medicine preparation is adopted for external application, preparing is easy, dosage control is easy, and compared with Western medicine treatment, the external traditional Chinese medicine composition has the advantages of being stable, lasting, not prone to medicine resistance, low in toxic and side effect, capable of obviously reducing complications and the like.

The invention relates to a visual hydrothorax and ascites injection and suction device and an auxiliary device thereof. The auxiliary device comprises that a flexible endoscope embedded with an optical fiber lighting unit is arranged inside a puncture trocar for long-distance observation and real-time shooting, so that medical care personnel can in real time observe puncture position and conditions as well as surgical operation position and conditions; a needle tip is provided with a certain inclined surface to avoid secondary injury to punctured positions due to inclination or movement of a sharp puncture needle; under guidance of real-time images, complications caused by conventional blind puncture can be avoided; meanwhile, ultra-micro optical fiber imaging can avoid radiation influenceand acoustic channel limitation and be applicable to bedside operation, thereby providing a safe and reliable technical means for manual hydrothorax and ascites injection and suction. The visual hydrothorax and ascites injection and suction device is simple in structure, reasonable in design, accurate in puncture positioning, capable of saving operation time and reducing injury of patients as well as operation risks, high in practicality and reliability and easy to generalize and apply.

The invention discloses a traditional Chinese medicine compound preparation used for treating nephrotic syndrome hypoproteinemia. Nephritic syndrome hypoproteinemia has clinical indications of plasma-albumin lower than 30g / L, lower extremities edema and hydrothorax and ascites with different degrees, poor appetite, hypodynamia, abdominal distention; or companied skin eczema, and bitter taste and dryness of mouths; or companied purplish mouth, lips or tongues, and purplish and expended sublingual venae. The compound preparation provides functions of spleen strengthening, kidney benefiting, blood circulation promoting, meridian obstruction removing, heat clearing, and dampness eliminating. The compound preparation is an oral preparation composed of an active component extract and medicinal auxiliary materials. The active component extract is at least extracted from black soybean, Mongolian milkvetch root, common yam rhizome, swordlike atractylodes rhizome, ligusticum, and leeches. The invention also discloses an extraction method of the active components and an application thereof in preparing medicines used for preventing and treating nephrotic syndrome hypoproteinemia.

The invention provides a preparation method for thyroid and mammary fine needle aspiration cell tissue blocks. The method includes the following steps: a, performing centrifugation on hydrothorax or ascites in advance, and obtaining hydrothorax or ascites supernatant; b, taking the hydrothorax or ascites supernatant to inject into a centrifuge tube; c, injecting a fine needle punctured thyroid andmammary specimen into the centrifuge tube, and then immediately taking 95% of ethanol to inject into the centrifuge tube; and d, performing centrifugation on the centrifuge tube and abandoning the supernatant so that cell sediment can be obtained, adding 4% of neutral buffer formaldehyde fixative, and adopting a paraffin tissue specimen processing program to perform conventional embedding after the cell sediment in the centrifuge tube is solidified. The preparation method can guarantee that tissue fragments and free-floating single cells are not lost; and cytoplasm is good in form and structure preservation, can be sliced continuously or for many times, and is suitable for a plurality of dyeing, and therefore, diagnosis efficacy can be enhanced.

The invention belongs to the filed of microorganism animal cell lines and provides a Chinese lung adenocarcinoma cell line with high metastases potentiality of bone, lever and salivary gland. According to the invention, hydrothorax of a lung adenocarcinoma first-diagnosis patient is used as primary cells for culturing; the human lung adenocarcinoma cell has the preservation number of CGMCC No.3138 and classified name as human lung adenocarcinoma cell line CPA-Yang4, with the tumorigenesis rate up to 50-100 percent; and the human lung adenocarcinoma cell can grow quickly and metabolize vigorously and has the biological characteristics of mainly transferring the bone, transferring the lever and the salivary gland and other organs as well as classic pathological characteristics of transferring tumor CPA-Yang4 bone and salivary gland. The invention can be used for providing reference data to early diagnosis for transferring the human lung adenocarcinoma bone and further analyzing lung cancer transferred relevant functional genomes and transferred relevant genes and can provide an effective molecule detection method for early diagnosis and curative effect assessment for bone transfer.

The invention relates to a traditional Chinese medicine composition for treating cancer osseous metastasis. The traditional Chinese medicine composition is prepared from, by weight, 8 parts to 14 parts of cassia twigs, 6 parts to 10 parts of radix paeoniae alba, 6 parts to 10 parts of liquorice, 30 parts to 60 parts of radix clematidis, 8 parts to 14 parts of radix bupleuri, 8 parts to 14 parts of scutellaria baicalensis, 8 parts to 14 parts of pericarpium citri reticulatae, 8 parts to 14 parts of curcuma aromatica, 8 parts to 14 parts of cortex moutan, 8 parts to 14 parts of notopterygium roots, 8 parts to 14 parts of radix angelicae pubescentis, 8 parts to 14 parts of radix saposhnikoviae, 1 part to 5 parts of asarum sieboldii, 8 parts to 14 parts of poria cocos, 8 parts to 14 parts of angelica sinensis, 8 parts to 14 parts of radix achyranthis bidentatae, 8 parts to 14 parts of dipsacus asperoides, 4 parts to 8 parts of rheum officinale, 8 parts to 14 parts of radices ligustici sinensis, 8 parts to 14 parts of chastetree fruits and 8 parts to 14 parts of ligusticum wallichii. The traditional Chinese medicine composition has the obvious inhibiting effect and the obvious repairing effect on various cancer patients, various metastatic tumor patients, various cancer hydrothorax and ascites patients and various osseous metastasis patients, and the anti-tumor mode achieves the positive and beneficial effect on improving the life quality and prolonging the lifetime of tumor patients.