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Preparation method for thyroid and mammary fine needle aspiration cell tissue blocks

A technology of cell tissue and thyroid, which is applied in the field of preparation of thyroid and breast fine-needle aspiration cell tissue blocks, which can solve the problems of unsuitable fine-needle aspiration mammary gland specimen cell smears and difficult accurate diagnosis, achieving good application effect and saving detection costs , improve the effect of diagnosis

Active Publication Date: 2019-07-05
马晓丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for preparing thyroid and breast fine-needle aspiration cell tissue blocks, so as to solve the problem that the existing tissue block preparation methods are not suitable for fine-needle aspiration of thyroid and breast specimens and cell smears, which are difficult to diagnose accurately

Method used

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  • Preparation method for thyroid and mammary fine needle aspiration cell tissue blocks
  • Preparation method for thyroid and mammary fine needle aspiration cell tissue blocks
  • Preparation method for thyroid and mammary fine needle aspiration cell tissue blocks

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1: Preparation of Thyroid Fine Needle Aspiration Cell Tissue Block.

[0026] 1. Pre-experimental steps: Take non-infectious pleural effusion and centrifuge twice to obtain 100mL of supernatant, respectively take 0.5mL, 1mL, 1.5mL, and 2mL of supernatant and inject them into disposable 10mL centrifuge tubes, add 95% of the same volume Gently mix with ethanol, centrifuge at 2000 r / min for 10 min, observe the size of the precipitated mass, and take the mass at the bottom of the centrifuge tube with a height of about 0.3cm (volume is about 1.0cm*1.0cm*0.3cm) It is used as a sample for backup, and the lumps of other centrifuge tubes are discarded.

[0027] Discard the supernatant of the selected sample, and add 5-10 times of 4% neutral buffered formaldehyde fixative. After solidification, the solidified cell pellets in the centrifuge tube were taken out and put into embedding paper, and then put into a dehydrated embedding box. Paraffin tissue specimens were process...

Embodiment 2

[0032] Example 2: Preparation of mammary fine needle aspiration cell tissue block.

[0033] 1. Pre-experimental steps: Take non-infectious ascites and centrifuge twice to obtain 100mL of supernatant, respectively take 0.5mL, 1mL, 1.5mL, and 2mL of supernatant and inject them into disposable 10mL centrifuge tubes, add 95% of the same volume Gently mix with ethanol, centrifuge at 2000 r / min for 5 minutes, observe the size of the precipitated mass, and take the mass at the bottom of the centrifuge tube with a height of about 0.3cm (volume is about 1.0cm*1.0cm*0.3cm) It is used as a sample for backup, and the lumps of other centrifuge tubes are discarded.

[0034] Discard the supernatant of the selected sample, and add 5-10 times of 4% neutral buffered formaldehyde fixative. After solidification, the solidified cell pellets in the centrifuge tube were taken out and put into embedding paper, and then put into a dehydrated embedding box. Paraffin tissue specimens were processed by ...

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Abstract

The invention provides a preparation method for thyroid and mammary fine needle aspiration cell tissue blocks. The method includes the following steps: a, performing centrifugation on hydrothorax or ascites in advance, and obtaining hydrothorax or ascites supernatant; b, taking the hydrothorax or ascites supernatant to inject into a centrifuge tube; c, injecting a fine needle punctured thyroid andmammary specimen into the centrifuge tube, and then immediately taking 95% of ethanol to inject into the centrifuge tube; and d, performing centrifugation on the centrifuge tube and abandoning the supernatant so that cell sediment can be obtained, adding 4% of neutral buffer formaldehyde fixative, and adopting a paraffin tissue specimen processing program to perform conventional embedding after the cell sediment in the centrifuge tube is solidified. The preparation method can guarantee that tissue fragments and free-floating single cells are not lost; and cytoplasm is good in form and structure preservation, can be sliced continuously or for many times, and is suitable for a plurality of dyeing, and therefore, diagnosis efficacy can be enhanced.

Description

technical field [0001] The invention relates to the technical field of cell tissue block preparation, in particular to a method for preparing thyroid and mammary gland cell tissue block by fine needle aspiration. Background technique [0002] The incidence of thyroid cancer and breast cancer worldwide is increasing rapidly year by year, among which the incidence of thyroid cancer is increasing at a rate of 6% per year. At present, the average incidence rate of thyroid cancer in China is 7.7 / 100,000, ranking fifth in the incidence rate of malignant tumors. 85% of thyroid adenocarcinomas are papillary carcinoma (Papillary Thyroid Carcinoma, PTC). A special biological behavior of PTC is the early occurrence of regional lymph node metastasis. Although the prognosis of differentiated thyroid cancer is good, about 30% of thyroid cancer patients will relapse during nearly 30 years of follow-up, and a small number of patients will eventually die of thyroid cancer. The preoperativ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/36
CPCG01N1/30G01N1/36
Inventor 马晓丽
Owner 马晓丽
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