Traditional Chinese medicine composition for treating cancer osseous metastasis
A technology of bone metastasis and composition, applied in the field of medicine, can solve the problems of inability to relieve cancer pain, different pathogenesis, etc., and achieve the effect of prolonging the survival period and improving the quality of life
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[0012] Weigh 11 grams of Guizhi, 8 grams of white peony, 8 grams of licorice, 45 grams of Clematis, 12 grams of Bupleurum, 11 grams of Scutellaria baicalensis, 11 grams of tangerine peel, 11 grams of turmeric, 11 grams of paeonol, 11 grams of notopterygium, 11g, Fangfeng 11g, Asarum 3g, Poria 11g, Angelica 11g, Achyranthes bidentata 11g, Chuanduan 11g, Rhubarb 6g, Ligusticum edulis 11g, Vitex 11g and Chuanxiong 11g, crushed , passed through a 100-mesh sieve, and mixed uniformly to obtain a powder.
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[0014] MTT (purchased from Sigma Company) was dissolved in normal saline, prepared into a working solution of 5 mg / mL, and stored at -20°C; dimethylsulfoxide (DMSO) was produced by Xi'an Chemical Reagent Factory and was analytically pure. Human breast cancer T47D cells were purchased from Shanghai Fengshou Biotechnology Co., Ltd.
[0015] Principle of MTT method
[0016] MTT is an acceptable H + yellow dye. In the mitochondrial respiratory chain of living cells, succinate dehydrogenase and cytochrome C can cleave the tetrazolium ring of MTT to produce blue-purple formazan crystals. Dead cells do not contain this dehydrogenase. The amount of Formazan crystals generated is directly proportional to the number of viable cells, and the DMSO solution of the crystals has a maximum absorption peak at 570nm. Therefore, the effect of the drug on cell proliferation can be evaluated by detecting the amount of formazan crystals.
[0017] Human breast cancer T47D cells were selected as ...
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