66 results about "Succinate dehydrogenase" patented technology

A microorganism which has an L-amino acid producing ability and has been modified so that succinate dehydrogenase activity and α-ketoglutarate dehydrogenase activity are decreased is cultured in a medium to produce and accumulate an L-amino acid in the medium or cells of the microorganism, and the L-amino acid is collected from the medium or cells to produce the L-amino acid.

A method for constructing an ALA production bacterial strain, the method enhances the activity of related enzymes promoting the synthesis of oxaloacetate and in the 5-aminolevulinic acid (ALA) production bacterial strain, or introducing exogenous related enzymes promoting the synthesis of oxaloacetate, such as phosphoenolpyruvate carboxylase or pyruvate carboxylase, and/or reducing the activity of related enzymes in the downstream metabolic pathway of succinyl coenzyme A in the bacterial strain, such as succinyl coenzyme A synthetase or succinate dehydrogenase, and/or reducing the activity of phosphoenolpyruvate carboxylated kinase and/or malic enzyme. An ALA high-yield bacterial strain constructed by utilizing the method, and method for utilizing the bacterial strain to prepare ALA.

The present invention provides a method for producing succinic acid using a yeast belonging to the genus Yarrowia, which has been modified to reduce activity of succinate dehydrogenase in said yeast.

The invention aims at establishing a method for quickly measuring total number of live bacteria of luminous bacteria, in particular to a method for counting the live bacteria based on the MTT coloration principle. The invention adopts MTT colorimetric method to measure the viable count of the luminous bacteria and carries out optimizing selection on the detection condition of the MTT colorimetric method. Therefore, the optimal method for measuring total number of the live bacteria of the luminous bacteria is determined. In the invention, first, the product of formazan generated from the reaction of succinate dehydrogenase in MTT and live thalli is dissolved in DMSO, light absorption value is determined within the range of 400nm-800nm, and the largest detected absorption wavelength is determined to be 500nm. By optimizing time and influencing the light absorption value by temperature, the optimal measurement condition is detected: the reaction time is 2h, and the reaction temperature is 25 DEG C. The invention has novel detection principle, simple and fast detection method, good repeatability, can be used for the quantitative detection of the number of the live bacteria of the luminous bacteria in the fields of food hygiene and safety, environmental monitoring and the like. The invention can also be applied to the tests of microbiology, immunology and other disciplines and used for measurement of the activity and number of the live bacteria materials. The invention can also develop the method for measuring the total number of the bacteria which is used for replacing the traditional method for measuring the total number of the live bacteria.

The invention discloses an allele of a succinate dehydrogenase B subunit gene sdHB of Corynespora cassiicola and a use thereof. The invention provides a protein. The protein is obtained through mutation of a succinate dehydrogenase B subunit amino acid I at 280th site in the amino acid sequence into V, wherein other amino acid residues are not changed. Novel unreported allele mutation is found in the Corynespora cassiicola group collected in the Liaoning province and can cause high resistance of the strain to fluopyram. The mutation has the important guiding significance for detection of SDHIs resistance in the field and the later drug resistance management.

Provided are a recombinant strain for preparing a terpenoid, and method for constructing the recombinant strain. Also provided is a recombinant bacterium 1, the recombinant bacterium 1 being a recombinant bacterium obtained in order to improve the enzymatic activity of α-ketoglutarate dehydrogenase in escherichia coli or the mutant thereof. The method for improving the enzymatic activity of α-ketoglutarate dehydrogenase in escherichia coli or the mutant thereof is replacing the original regulating element of the ketoglutarate dehydrogenase gene (sucAB) in escherichia coli or the mutant thereof with any of the following regulating elements: artificial regulating element M1-46, M1-37, and M1-93. Also provided are a plurality of recombinant bacteria. By improving the enzymatic activity of α-ketoglutarate dehydrogenase, succinic acid dehydrogenase and transaldolase therein and improving the ability of a cell to synthesize NADPH and ATP, the efficiency of the MEP pathway and the production capacity of terpenoid are improved.

A composition containing succinate dehydrogenase inhibitor and a potentiator has been discovered to enhance the activity of the succinate dehydrogenase such that the amount of the succinate dehydrogenase inhibitor need to effectively treat a microbial substance can be reduced substantially. The compositions may be used as additives for paints and coatings, and protecting crops, seeds, wallboard, metal working fluids, wood from mold, fungi and other microbes.

The invention relates to a pleurotus eryngii carboxin resistance screening and transforming vector as well as a construction method and application thereof. A DNA fragment of succinate dehydrogenase iron sulfur subunit with point mutation is ligated into a plasmid vector, and is denoted as pTSdi. The transforming vector provided by the invention can be used for transforming pleurotus eryngii so asto enable the pleurotus eryngii to obtain the resistance to a fungicide-carboxin; in addition, an enzyme cleavage site for inserting an exogenous gene fragment is reserved in the constructed transforming vector, so that an interested exogenous gene can be conveniently inserted into the transforming vector and transformed into the pleurotus eryngii for performing genetic research; therefore, the pleurotus eryngii carboxin resistance screening and transforming vector has a good application prospect.

The invention relates to a genetic transformation selection marker of Pleurotus eryngii. The selection marker is obtained by a single base mutation of a succinate dehydrogenase iron sulfur subunit gene encoded by the genome of Pleurotus eryngii. The carboxin resistance selection marker obtained by the invention is derived from a single base mutation of a self-gene of Pleurotus eryngii with no introduction of foreign DNA of other species, and has high safety. Meanwhile, the selection marker has high transformation efficiency and can replace a hygromycin resistance screening marker commonly usedin the genetic transformation of Pleurotus eryngii. The selection marker obtained by the invention can be safely and efficiently applied to the genetic improvement of Pleurotus eryngii, lays a foundation for cultivating new varieties of Pleurotus eryngii which accords with the production demand and consumer preferences, and has a good market application prospect.

The invention discloses a sterilization composition and application thereof and relates to the technical field of pesticide mixtures. The sterilization composition is prepared from effectively ingredients including phenazino A and succinate dehydrogenase inhibitor bactericide B, wherein succinate dehydrogenase inhibitor bactericide B is one or more of fluopyram, bicycle-fluxapyroxad penflufen and the like. The weight ratio of phenazino A to succinate dehydrogenase inhibitor bactericide B is 1:100-100:1. The effective ingredients A and B are blended to achieve remarkable synergism, and the application amount of single agents can be effectively reduced. Good effects on enlarging the bacteriocidal spectrum and delaying plant drug resistance are achieved, the lasting period can be prolonged, and obvious popularization value is achieved. The composition can be added with other auxiliary ingredients allowing to be used in pesticide to be prepared into any dosage forms.

The invention discloses a reagent for detecting whether gene mutation of mitochondrial succinate dehydrogenase (SDH) exists in samples and a kit containing the reagent. The reagent or the kit can be used for susceptibility analysis of pheochromocytoma. The invention also discloses an optimized method by which SDH gene amplification products are obtained and a method for determining whether gene mutation of SDH exists in the nucleic acid samples to be tested.

The invention discloses a traditional Chinese medicine composite used for releasing fatigue as well as preparation method of the traditional Chinese medicine composite. The traditional Chinese medicine composite disclosed by the invention takes Maca which has various functions of improving fertility, resisting fatigue, improving sexual performance, antioxidation, inhibiting hyperplasia of prostate, alleviating menopausal syndrome, strengthening immunity combined with American ginseng extract, rhizome chuanxiong extract, morinda officinalis extract, tuber onion seed extract and tangerine peel extract. The traditional Chinese medicine compound has the functions of antioxidation and antifatigue, can lower the levels of lactate dehydrogenase and serum urea, improve succinate dehydrogenase activity of muscles, effectively relief uncomfortable feeling after exercise, such as limb weakness, tiredness and muscle soreness. The invention also provides a traditional Chinese medicine preparation containing the traditional Chinese medicine composite and a preparation method of the traditional Chinese medicine preparation.

The invention relates to application of CART (Cocaine Amphetamine Regulated Transcript) in preparing medicaments for treating alzheimers diseases. In the invention, CART is prepared into injection preparations for treating alzheimers diseases, and the defects of the traditional medicaments for enhancing cholinergic action and N-methyl-D-aspartic acid (NMDA) receptor antagonists are overcome. The novel medicament has multiple target point actions on the generation and the degradation of A beta as well as the influence of the neurotoxicity and the molecular mechanism of the A beta. The invention has effects on effectively treating dementia with estrogen and up-regulating intracephalic CART; more CARTs are distributed in intracephalic hippocampus; CART exists in intracephalic acetylcholine neurons and can promote the release of acetylcholine; and mitochondrial dysfunction induced by A beta neurotoxicity is the main pathway for the A beta to cause neuronal apoptosis. The CART has a directaction on mitochondrial succinodehydrogenase subunit B and can maintain the respiration of mitochondria and the generation of energy, alleviate oxidative stress, stabilize mitochondrial membrane potential and prevent the mitochondria from being damaged, thereby inhibiting apoptosis.