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Screening and application of biomarker related to AD cell model constructed by A beta induction

A technology of biomarkers and cell models, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of new biomarkers that have not been reported

Active Publication Date: 2021-09-03
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the development of novel biomarkers in AD cell models based on proteomics has not been reported

Method used

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  • Screening and application of biomarker related to AD cell model constructed by A beta induction

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Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0024] cell culture.

[0025] 1.1 Cell subculture: Before the experiment, the ultra-clean bench and the cells were irradiated with ultraviolet light for 30 minutes to sterilize, and ventilated for 10 minutes. Transfer the prepared complete culture solution, PBS, and trypsin from 4°C to room temperature. Use an alcohol cotton ball to wipe the operating table and light the alcohol lamp, take the culture bottle containing the cells out of the incubator, observe the color and clarity of the culture solution, and observe the cell shape, adhesion and fusion degree under a microscope. Reaching about 80% can be subcultured. Transfer the cells to the ultra-clean bench, suck out the culture medium, add PBS, shake the bottle body, suck off the PBS, wash repeatedly three times, then add trypsin, the cells can be seen to become round under the microscope, quickly suck out the trypsin, add complete culture solution to stop Digestion. After blowing the cells into a single-cell suspension,...

Embodiment approach 2

[0053] 2.1 Bioinformatics analysis.

[0054] The Panther Classification System was used to conduct GO enrichment analysis of the screened differential proteins, including biological processes, molecular functions and cellular components; the STRING analysis software was used to analyze the protein interaction of the differential proteins; the KEGG signaling pathway analysis was performed on the differential proteins.

[0055] Result analysis.

[0056] Gene Ontology (GO) enrichment analysis of differential proteins.

[0057] GO functional enrichment analysis was performed on the expressed proteins of the AD cell model and the normal control group in terms of cell biological process, cellular components and molecular functions. It was found that in the biological process of differential proteins, cellular process, single-organism process, metabolic process, single-organism cellular process, cellular metabolic process, etc. showed a relatively good enrichment effect; in cell com...

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Abstract

The invention belongs to the field of biomarkers, and particularly relates to screening and application of a biomarker related to an AD cell model constructed by A beta induction. The biomarker comprises one or a combination of more of the following components: Cysteine Desulfurase, mistoral, the code is NFS1, NADH (Nicotinamide Adenine Dinucleotide) dehydrogenase [ubiquitin] 1 alpha subcomplex subunit 9, mitochondrial, the code is NDUFA 9; 3-hydroxyacyl-CoA dehydrogenase type-2, and the code is HSD17B10; Cytochrome c oxidase subunit NDUFA4, and the code is NDUFA4; NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial, the code is NDUFS1; Succinate dehydrogenase[ubiquinone]iron-sulfur subunit, mitochondrial, the code is SDHB; NADH dehydrogenase[ubiquinone]iron-sulfur protein 4, mitochondrial, the code is NDUFS4; Lipoprotein lipase, the code is LPL; NADH dehydrogenase[ubiquinone]1 alpha subcomplex subunit 5, the code is NDUFA5. A proteomics method is adopted, a group of biomarkers are found in an A beta-induced AD cell model, and the biomarkers are found to be mutually matched in the A beta-induced AD cell model and jointly play an important role. The method for detecting the AD cell model constructed through A beta induction is enriched, and a new target is provided for clinical research.

Description

technical field [0001] The present invention relates to the field of biomarkers. Specifically, the present invention relates to the screening and application of biomarkers related to AD cell models induced by Aβ. Background technique [0002] Alzheimer's disease (AD) is the most common degenerative disease of the central nervous system, leading to cognitive decline, memory deficits, neuropsychiatric symptoms, etc., accounting for 50% to 75% of dementias. With the extension of human life span, the prevalence of AD doubles approximately every 5 years after the age of 65. Currently, the number of AD patients continues to rise, affecting more than 150 million people worldwide, which has brought a huge burden to families and society, and has become a global challenge to public health. But due to the complexity of its pathogenesis, there are currently no effective treatments to cure AD or prevent its progression. Therefore, the early diagnosis of AD has become the focus of AD p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 张林方德宇张晓丽柳春赵丹玉王艳杰陈文娜郭隽馥丛培玮谷丽艳殷晓梅倪菲李硕苗君静宋丹君朱昱林郭星池
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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