The invention discloses a method and a kit for rapidly detecting homovanillic acid, vanillylmandelic acid, 5-hydroxyindoleacetic acid and
creatinine in human
urine. The method comprises the following steps of 1) preparing a
sample dilution working solution, 2) under a dark condition, taking a
urine sample, adding EDTA and Na2S2O3 into the
urine sample, then adding
acetic acid for acidification, and uniformly mixing to obtain a to-be-detected
urine sample, and (3) mixing a to-be-detected
urine sample, a calibration product, a urine
quality control product and the sample
diluent working solution, sealing a film, vorticing, centrifuging, taking supernate, and detecting by adopting liquid
chromatography-
mass spectrometry. When a
urine sample prepared from random urine is detected, homovanillic acid, vanillylmandelic acid, 5-hydroxyindoleacetic acid and
creatinine can be quantified at a time only by 10 microliters of urine sample, the
pretreatment method is simple and rapid, the
operation time is saved,
neuroblastoma and carcinoid in a
human body can be effectively screened and diagnosed according to the indexes, and the
pheochromocytoma can be screened in an auxiliary manner.