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Method and kit for rapidly detecting homovanillic acid, vanillylmandelic acid, 5-hydroxyindoleacetic acid and creatinine in human urine

A technology of hydroxyindole acetic acid and vanillyl mandelic acid, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to accurately quantify analytes and high detection limits, and achieve a simple and fast pretreatment method, simple processing process, and economical The effect of operating time

Pending Publication Date: 2021-06-11
BGI CLINICAL LAB (SHENZHEN) CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the limitation of the method itself and the detector, high-performance liquid chromatography can only measure one or several analytes, and the detection limit of the method itself is high, so it cannot accurately quantify the analytes with extremely low content

Method used

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  • Method and kit for rapidly detecting homovanillic acid, vanillylmandelic acid, 5-hydroxyindoleacetic acid and creatinine in human urine
  • Method and kit for rapidly detecting homovanillic acid, vanillylmandelic acid, 5-hydroxyindoleacetic acid and creatinine in human urine
  • Method and kit for rapidly detecting homovanillic acid, vanillylmandelic acid, 5-hydroxyindoleacetic acid and creatinine in human urine

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Embodiment 1

[0101] Example 1. Rapid Detection Kit for Homovanillic Acid, Vanillylmandelic Acid, 5-Hydroxyindoleacetic Acid and Creatinine in Human Urine

[0102] The rapid detection indicators of the kit provided in this embodiment include: vanillylmandelic acid (VMA), homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA) and creatinine (Cr), wherein the amount of creatinine is taken as random urine of normalization.

[0103] The contents of the rapid test kit include:

[0104]

[0105]

[0106] In this kit, Set A is stored at room temperature, and Set B is stored at a temperature below -18°C.

[0107]Among them, calibrators: there are seven calibrators in sequence, Cal1-Cal7, each 50 μL, and the calibrators are artificial urine samples; the concentration of the homovanillic acid standard in the Cal1 artificial urine samples is 0.5 μg / ml, The concentration of vanillylmandelic acid is 0.5 μg / ml, the concentration of 5-hydroxyindoleacetic acid is 0.5 μg / ml, and the concentrat...

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Abstract

The invention discloses a method and a kit for rapidly detecting homovanillic acid, vanillylmandelic acid, 5-hydroxyindoleacetic acid and creatinine in human urine. The method comprises the following steps of 1) preparing a sample dilution working solution, 2) under a dark condition, taking a urine sample, adding EDTA and Na2S2O3 into the urine sample, then adding acetic acid for acidification, and uniformly mixing to obtain a to-be-detected urine sample, and (3) mixing a to-be-detected urine sample, a calibration product, a urine quality control product and the sample diluent working solution, sealing a film, vorticing, centrifuging, taking supernate, and detecting by adopting liquid chromatography-mass spectrometry. When a urine sample prepared from random urine is detected, homovanillic acid, vanillylmandelic acid, 5-hydroxyindoleacetic acid and creatinine can be quantified at a time only by 10 microliters of urine sample, the pretreatment method is simple and rapid, the operation time is saved, neuroblastoma and carcinoid in a human body can be effectively screened and diagnosed according to the indexes, and the pheochromocytoma can be screened in an auxiliary manner.

Description

technical field [0001] The invention belongs to the field of drug detection, in particular to a method for rapidly detecting homovanillic acid, vanillylmandelic acid, 5-hydroxyindoleacetic acid and creatinine in human urine and a kit thereof. Background technique [0002] Neuroblastoma (Neuro blastoma, NB) is an endocrine tumor, and is the most common malignant solid tumor among non-central nervous system tumors in childhood, accounting for about 8%-10% of all childhood tumors. Its typical clinical manifestations are fever, protruding eyes, ecchymosis around the eyes, bone pain, and abdominal mass, etc. [1] ; A small number of children with NB are characterized by typical symptoms of other systems as the first manifestation, such as acute cerebellar ataxia, allergic purpura and idiopathic thrombocytopenic purpura, etc., which are called paraneoplastic syndromes. [0003] Pheochromocytoma is a type of endocrine tumor originating from neuroectodermal chromaffin tissue, its ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 李梓僮廖云莉饶维桥任艳訾金林梁
Owner BGI CLINICAL LAB (SHENZHEN) CO LTD
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