Chromogranin A detection reagent, detection reference interval and detection method

Abstract

The invention discloses a chromogranin A detection reagent. The chromogranin A detection reagent comprises an immune complex and an antibody detection system, wherein the immune complex is formed by compounding a marked first specific antibody, a marked second specific antibody, a chromogranin A calibration product, a chromogranin A quality control product and a sample to be detected; the first specific antibody and the second specific antibody are formed by carrying out epitope specific binding on antibodies and amino acid sites in a human chromogranin A amino acid sequence; the amino acid sites comprise: 1st to 113rd sites, 131st to 143rd sites, 337th to 364th sites and 373rd to 439th sites; the detection reagent can be used for quantitatively detecting the concentration of chromograninA in human blood plasma and is clinically mainly used for auxiliary diagnosis and differential diagnosis on pheochromocytoma and neuroendocrine neoplasm; the reference interval of the invention has ahigh conformity degree with clinical diagnosis, and the accuracy of the auxiliary diagnosis is improved; a detection method developed by a full-automatic magnetic bead chemiluminescence platform is further utilized by the invention and the detection period is shortened.

Description

technical field [0001] The invention belongs to the field of reagent diagnosis research, in particular to a chromogranin A detection reagent, a detection reference interval and a detection method. Background technique [0002] Chromogranin A or chromogranin A (CGA) belongs to the chromogranin protein family. Chromogranin exists in the secretory vesicles of all neuroendocrine cells that can secrete catecholamines or other endocrine hormones, and is rich in acidic amino acids. Acidic soluble protein, originally extracted from bovine adrenal medulla granules, was found to widely exist in the nervous system and APUD (amine precursor uptake and decarboxylation) system. cDNA sequence analysis and biochemical research show that CGA is composed of 439 amino acid residues, its molecular weight is 48KD, its isoelectric point is 4.8, and it has the characteristics of heat deformation resistance. [0003] As a precursor peptide, CGA can be decomposed into different fragments in differe...

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Problems solved by technology

[0009] However, due to the inherent characteristics of the methodology, the above listed products all have certain defects.

For example, the enzyme-linked immunoassay method has cumbersome operation steps and takes a long time to react; the immunoradiometric assay method has poor sensitivity, cannot be absolutely quantified, and there is radiation and pollution; the fluorescent immunoassay method has poor sensitivity and the test results are affected by subjective factors

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