40 results about "Platycodin D" patented technology

The invention relates to a saponin with immune adjuvant function and a preparation method thereof, a vaccine preparation containing the saponin as an adjuvant, and applications of the saponin and the vaccine preparation in the prevention and treatment of infectious diseases and cancers of human and animals. The saponin is platycodin D, platycodin D2, or a total-saponin containing the two saponin compounds. The platycodin D and platycodin D2 are both extracted and separated from balloonflower, a Chinese medicine. The saponin can induce an organism to generate Th1-type and Th2-type immune responses, show the capability of inducing the organism to generate stronger cell immune response and humoral immune response to a vaccine than the alhydrogel adjuvant known in the prior art, and can be taken as the immune adjuvant for a plurality of vaccines and achieve an ideal immunity effect. The vaccine which takes the saponin as the adjuvant has simple preparation technology and simple and convenient method, and the quality is easy to control and the saponin can be reserved by freezing.

The invention provides a preparation method and a new application of a platycodon grandiflorum active site, in particular the application of a platycodon grandiflorum active site in an antineoplastic drug. The invention also relates to two new triterpenoidal saponin compounds (compounds A and B) separated from the platycodon grandiflorum active site by a phytochemical separation way and a preparation method thereof. The invention further provides a detection method for QC (Quality Control) of the platycodon grandiflorum by platycodin D, polygala saponin D2, platycodin D3, deapiose platycodin D3, platycodin D2, deapiose platycodin D2, deapiose platycodin E and platycodin E, and the method has excellent repeatability and is capable of comprehensively evaluating the quality of the platycodon grandiflorum, platycodon grandiflorum extracts and the platycodon grandiflorum active site.

The invention provides a non-irritant contraceptive gel with a function of inhibiting bacteria and a preparation method thereof, relates to contraceptive gel and a preparation method thereof, and aims to improve the nonoxinol spermicidal effect and solve the problem of side effect caused when existing nonoxinol is used for killing sperms. The gel is prepared from the following main components: chlorhexidine acetate, aloe gel, nonoxinol, pomegranate powder, turmeric extract and platycodin D. The method comprises the following steps of: 1, adding water into Carbomer to swell to obtain a Carbomer solution; regulating the pH value to obtain gel; 2, dissolving aloe gel with water to obtain aloe gel solution; and 3, adding ethanol and tween 80 into chlorhexidine acetate, uniformly stirring to obtain a mixed solution, adding pure water, uniformly stirring, adding into gel and nonoxinol to obtain mixture; and 4, adding pomegranate powder, turmeric extract and platycodin D, and uniformly mixing to obtain a contraceptive gel. The contraceptive gel has a good sperm killing effect, does not have side effect if being used for a long time, and is used in the field of contraceptive medicines.

The invention provides a method for extracting platycodin D from platycodon grandiflorum and aims to mainly solve the problems that the prior art is complex to operate, low in product content, low in recycle rate and unsuitable for large-scale production operation. The method for extracting platycodin D from the platycodon grandiflorum comprises the following steps: (1) extraction; (2) primary ultrafiltration; (3) secondary nanofiltration; and (4) recrystallization. The method for extracting platycodin D from the platycodon grandiflorum can be used for preparing a platycodin D product of which the purity is higher than 98% by just a plurality of steps, and is convenient to operate, high in product content, high in recycle rate, small in harms caused by solvents and suitable for large-scale production operation.

The invention belongs to a preparation method for platycodin D, and adopts a mixed bacterial strain which includes a Pichia kudriavzevii bacterial strain and a Bacillus velezensis bacterial strain toprepare the platycodin D. According to the preparation method, two types of bacterial strains with different conversion mechanisms are used for the first time to convert platycodin in a mixed fermentation way, and the yield of the platycodin D and deapioplatycodin D can be effectively improved. By use of bacterial strain fermentation characteristics, the platycodin can be subjected to biological transformation, the safe and environmentally-friendly production of the platycodin can be realized, platycodon grandiflorum resources can be fully utilized, and the platycodin which is contained in theplatycodon grandiflorum resources and has low biological activity can be converted into saponin with high biological activity so as to achieve an effect of recycling waste materials.

The present invention relates to cosmetic and pharmaceutical compositions used for whitening skin which include platycodin-D as an effective constituent. The platycodin-D can be used safely without side effect to skin, and has an excellent effect for restraining melanin from generating and preventing pigmentation. Therefore, the compositions using platycodin-D as an effective constituent can be used effectively for releasing splashes and flecks, and whitening skin.

The invention discloses a medicament for killing sperms in vitro, which comprises the raw materials in percentage by weight: 3-7mg of platycodin D, 3-5mg of divaricate saposhnikovia extract, 3-5mg of cassia twig extract, 1-3mg of malaytea scurfpea extract and 130-170ml of 0.9 percent physiological saline. The platycodin D, the divaricate saposhnikovia extract, the cassia twig extract, and the malaytea scurfpea extract are added into a vessel for uniformly mixing and then 130-170ml of 0.9 percent physiological saline is added into the vessel for uniformly shaking to obtain the medicament. The medicament is prepared from pure traditional Chinese medicine, can rapidly achieve the aim of killing the sperms in vitro and has better antimicrobial effect and sterilizing effect. The medicament can be prepared into emulsion, suppository and other forms for external contraception of vagina, or can be prepared into sustained-release capsules which are placed in an intrauterine device and are carried to enter a human body, so that the contraception effect of the intrauterine device can be enhanced and no toxic and side effect are generated.

The invention relates to a traditional Chinese medicine compound preparation for treating ovarian tumor and application thereof. The traditional Chinese medicine compound preparation is prepared by using an alcohol extract of radix notoginseng, spreading hedyotis herb and herba lycopi, platycodin D and geniposide as active ingredients, wherein the alcohol extract is prepared according to the following steps: weighing 20 to 30 parts of radix notoginseng, 20 to 30 parts of spreading hedyotis herb and 5 to 10 parts of herba lycopi, crushing, adding 5 to 10 times of ethyl alcohol with volume fraction of 80% to 90%, soaking for 10 to 20 hours at room temperature, refluxing in a water bath of 80 DEG C to 90 DEG C for two to three times, each time for 1 to 3 h, then combining filtrate, concentrating the filtrate into an extracting solution with the concentration of 1 to 2g / ml, and drying the extracting solution at 40 DEG C to 50 DEG C to be constant in weight. The traditional Chinese medicinecompound preparation provided by the invention has the advantages that tumor growth can be significantly inhibited, the platycodin D and the geniposide have certain synergistic effects, less medicinematerials are used, the medicine efficacy is specialized, the safety is high, and the development and promotion of clinical medicine is facilitated.

The invention relates to a traditional Chinese medicine compound preparation for treating ovarian tumor and application thereof. The traditional Chinese medicine compound preparation is prepared by using an alcohol extract of radix notoginseng, spreading hedyotis herb and herba lycopi, platycodin D and geniposide as active ingredients, wherein the alcohol extract is prepared according to the following steps: weighing 20 to 30 parts of radix notoginseng, 20 to 30 parts of spreading hedyotis herb and 5 to 10 parts of herba lycopi, crushing, adding 5 to 10 times of ethyl alcohol with volume fraction of 80% to 90%, soaking for 10 to 20 hours at room temperature, refluxing in a water bath of 80 DEG C to 90 DEG C for two to three times, each time for 1 to 3 h, then combining filtrate, concentrating the filtrate into an extracting solution with the concentration of 1 to 2g / ml, and drying the extracting solution at 40 DEG C to 50 DEG C to be constant in weight. The traditional Chinese medicinecompound preparation provided by the invention has the advantages that tumor growth can be significantly inhibited, the platycodin D and the geniposide have certain synergistic effects, less medicinematerials are used, the medicine efficacy is specialized, the safety is high, and the development and promotion of clinical medicine is facilitated.

The invention relates to a saponin with immune adjuvant function and a preparation method thereof, a vaccine preparation containing the saponin as an adjuvant, and applications of the saponin and the vaccine preparation in the prevention and treatment of infectious diseases and cancers of human and animals. The saponin is platycodin D, platycodin D2, or a total-saponin containing the two saponin compounds. The platycodin D and platycodin D2 are both extracted and separated from balloonflower, a Chinese medicine. The saponin can induce an organism to generate Th1-type and Th2-type immune responses, show the capability of inducing the organism to generate stronger cell immune response and humoral immune response to a vaccine than the alhydrogel adjuvant known in the prior art, and can be taken as the immune adjuvant for a plurality of vaccines and achieve an ideal immunity effect. The vaccine which takes the saponin as the adjuvant has simple preparation technology and simple and convenient method, and the quality is easy to control and the saponin can be reserved by freezing.

The present invention relates to cosmetic and pharmaceutical compositions which include platycodin D as an active ingredient. The platycodin D of the present invention can be used safely and without side effects on the skin, and also has outstanding effects in suppressing the production of melanin and impeding pigment deposition, and thus a composition which contains the present invention as an active ingredient can be used very effectively to improve skin patches and freckles and for skin whitening.