Method for determining content of platycodin D in Huang's sound tea

A technology for Platycodon grandiflorum saponins and Huang's sing, which is applied in the field of detection of active ingredients of medicines, can solve the problems of lack of a method for determining the content of platycodon saponins D in Huang's-sound tea, and achieves a simple and efficient extraction method, short detection time, and high inspection efficiency. Effect

Inactive Publication Date: 2021-09-24
WUXI JIMIN KEXIN SHANHE PHARMA
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The prior art lacks the method for determining ...

Method used

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  • Method for determining content of platycodin D in Huang's sound tea
  • Method for determining content of platycodin D in Huang's sound tea
  • Method for determining content of platycodin D in Huang's sound tea

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Embodiment 1

[0049] 1) Reference substance solution: Take an appropriate amount of platycodon saponin D reference substance, weigh it accurately, add methanol to make platycodon saponin D reference substance solution with three concentration levels of low, middle and high, and obtain it.

[0050] 2) The test solution: take 2g of Huang’s Xiangsheng tea, weigh it accurately, add 30ml of methanol, sonicate (power 250W, frequency 50Hz, water bath temperature below 30°C) for 1h, filter, wash the filter residue with an appropriate amount of methanol, and combine The filtrate and lotion were evaporated to dryness on a warm water bath, the residue was dissolved in methanol and transferred to a 5ml volumetric flask, diluted to the mark with methanol, and shaken up to obtain the product.

[0051] 3) measure: get reference substance solution, each 20 μ l of need testing solution, inject high performance liquid chromatograph, obtain chromatogram, according to chromatogram peak area, calculate the conte...

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Abstract

The invention relates to a method for determining the content of platycodin D in Huang's sound tea, which comprises the following steps: 1) preparing a reference substance solution: taking a proper amount of a platycodin D reference substance, precisely weighing, and adding methanol to prepare the platycodin D reference substance solution; (2) preparing a test solution: taking Huang's sound tea, precisely weighing, adding methanol, dissolving, filtering, washing filter residues with a proper amount of methanol, combining filtrate and washing liquor, placing on a warm water bath to be volatilized, dissolving residues with methanol, and transferring into a measuring flask; and 3) determination: taking the reference substance solution and the test solution, injecting the reference substance solution and the test solution into a high performance liquid chromatograph to obtain a chromatogram, and calculating the content of platycodin D in the test solution according to the peak area of the chromatogram.

Description

technical field [0001] The invention relates to a method for detecting active ingredients of medicines, in particular to a method for determining the content of platycodon saponin D in Huang's Xiangsheng tea. Background technique [0002] Huang's sound tea, the name of Chinese patent medicine. It is composed of bellflower, mint, menthol, cicada slough, myrobalan meat, fat sea, fritillaria, catechu, chuanxiong, rhubarb (wine), forsythia, and licorice. It has the functions of relieving throat and opening sound, clearing away heat and resolving phlegm, reducing swelling and relieving pain. It is used for hoarseness, pain in vocalization, and dry throat caused by acute laryngitis and chronic laryngitis caused by wind-heat invading the lung. Platycodon grandiflorum in the prescription is a monarch drug, which can open lung qi, relieve throat and open sound, and its main component platycodon saponin D. The present invention adopts high performance liquid chromatography-evaporati...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/74G01N2030/324
Inventor 叶馨薇戴亚妮张小娜徐凤莲邱婷张萍
Owner WUXI JIMIN KEXIN SHANHE PHARMA
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