An isoflavone compound having antibacterial activity and a preparing method and applications thereof

A technology of antibacterial activity, compounds, applied in the field of phytochemistry

Active Publication Date: 2017-08-29
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • An isoflavone compound having antibacterial activity and a preparing method and applications thereof
  • An isoflavone compound having antibacterial activity and a preparing method and applications thereof
  • An isoflavone compound having antibacterial activity and a preparing method and applications thereof

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Embodiment 1

[0048] Preparation of isoflavone compound C 17 h 14 o 4 , including extracting extract, silica gel column chromatography, and high-pressure liquid chromatography separation, specifically adopting the following steps:

[0049] 1. Extraction of extract: get Pueraria root sample and pulverize to 30 mesh, use high-concentration methanol (w%: 95%) or high-concentration ethanol (w%: 95%) or high-concentration acetone (w%: 70%) as extraction solvent , extraction solvent: Radix Puerariae (weight ratio)=3:1, soak 54h each time, extract 4 times, combine extract, filter and concentrate to become extractum.

[0050] 2. Silica gel column chromatography: dissolve the extract with 2.5 times the weight of pure methanol or pure acetone, mix the sample with 1.2 times the weight of 80-100 mesh silica gel, and dry pack with 3 times the weight of 250 mesh silica gel The column is subjected to silica gel column chromatography; chloroform-acetone solution with a volume ratio of (1:0, 20:1, 9:1, 8...

Embodiment 2

[0054] Pueraria lobata samples were from Nanping, Guangxi, and the variety was Pueraria mirifica. Sampling 2.0 kg of kudzu root crushed to 30 mesh and soaking in methanol with a weight percentage concentration of 95% for extraction 5 times, each time for 24 hours, the weight ratio of the extraction solvent and kudzu root used each time is 2:1, the extracts are combined and filtered , Concentrated under reduced pressure into an extract to obtain 105 g of extract.

[0055] After dissolving the extract with 2.0 times the amount of pure methanol by weight, mix the sample with 120g of 100-mesh silica gel, pack 0.3kg of 160-mesh silica gel into a column for silica gel column chromatography, and use a volume ratio of 1:0, 20:1, 9:1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring Merge the same part, each gradient elution to TLC spot plate without spots (that is, after the gradient elution does not produce substances), replace the next gradient elution to...

Embodiment 3

[0058] The Pueraria lobata sample comes from Wanzhou, Chongqing, and the variety is Chaige. 3.5 kg of Pueraria lobata samples crushed to 30 meshes are sampled and chopped, soaked and extracted 4 times with 95% ethanol aqueous solution with a concentration of 95% by weight, each time for 48 hours. The weight ratio of the extraction solvent to Pueraria Radix was 3:1, the extracts were combined, filtered, and concentrated under reduced pressure to obtain an extract to obtain 250 g of the extract.

[0059] The extract is dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 250g of 80-mesh silica gel, and 1kg of 200-mesh silica gel is packed into a column for silica gel column chromatography. The volume ratio is 1:0, 20:1, 9 :1, 8:2, 7:3, 6:4, 1:1, 1:2 chloroform-acetone gradient elution, TLC monitoring and merging the same part, each gradient elution to the TLC point plate after no point ( That is, after the gradient elution does not produce substances)...

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Abstract

The invention relates to an isoflavone compound having antibacterial activity and a preparing method and applications thereof, and belongs to the technical field of phytochemistry. The structure of the compound is shown as a formula (I). The formula (I) is shown in the specification. The compound is prepared from kudzu vine root that is a traditional medicinal and edible plant and through steps of extractum extraction, column chromatography on silica gel and separation and purification through high performance liquid chromatography. Activity tests prove that the compound has a good bacteriostatic function; when the compound is added into a tobacco material solution, the compound can effectively inhibit microbe growth in the material solution and can prolong the shelf life of the solution; when the compound is used for cigarette tipping paper, the compound can eliminate or reduce the possibility of bacterium breeding and reproduction in the cigarette tipping paper; and the compound has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to an isoflavone compound with antibacterial activity obtained by extracting and separating traditional medicine and food homologous plant kudzu root, and meanwhile, the invention also relates to a preparation method and application of the compound. Background technique [0002] Pueraria lobata is the dried root of the leguminous plant Pueraria mirifica, commonly known as Pueraria mirifica. Excavated in autumn and winter, cut into thick slices or small pieces while fresh; dry. Sweet, pungent, cool. It has the functions of relieving muscle and reducing fever, clearing rash, promoting body fluid and quenching thirst, promoting yang and relieving diarrhea. It is often used for superficial syndromes of fever, stiff neck and back pain, opaque measles, fever and thirst, yin deficiency and thirst, heat diarrhea and dysentery, and diarrhea due to spleen deficiency. At ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/36C07D311/40A01N43/16A01P1/00A01P3/00D21H21/36
CPCA01N43/16C07D311/36C07D311/40D21H21/36
Inventor 李雪梅杨叶昆米其利向海英李晶刘欣夭建华徐济仓张承明孔维松许永周敏杨光宇胡秋芬李干鹏
Owner CHINA TOBACCO YUNNAN IND
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