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This invention discloses a flavonoid compound with antibacterial activity, its preparation method, and its application. It is prepared from *Hylocereus undatus* twigs through pretreatment, extract extraction, silica gel column chromatography, and high-performance liquid chromatography separation. The compound is named: 4'-hydroxy-8-methoxy-6-(4-methyl-1... H 4'-pyrrole-2-yl)-flavonoid, its English name is: 4'-hydroxy-8-methoxy-6-(4-methyl-1 H (-pyrrol-2-yl)-flavone, which has the following structure: The antibacterial active flavonoids of this invention are obtained from *Hypericum esculentum* twigs through extraction, silica gel column chromatography, and high-performance liquid chromatography. The isopentenyl flavonoids, after activity testing, show good antibacterial activity and a sweet aftertaste. Using this compound in cigarette tipping paper can eliminate or reduce the possibility of harmful bacteria growth and reproduction in the cigarette tipping paper and improve its flavor.