The invention discloses an environment-friendly and durable sisal bag and a preparation method thereof. The environment-friendly and durable sisal bag comprises raw materials in parts by weight as follows: sisal fibers, shengma fibers, ramie fibers, bamboo fibers, carbon fibers, banana fibers, konjac starch, polyvinyl alcohol, polyvinyl chloride, aromatic particles, negative ion mineral particles, thermoplastic polyurethane, titanium dioxide, laccase, agar, a plasticizer, a surfactant, a plant extract solution and an antibacterial agent. The natural degradable fibers including the sisal fibers, the shengma fibers, the ramie fibers, the bamboo fibers, the carbon fibers and the banana fibers are selected as the main raw materials, the plastic bag can be degraded rapidly in a natural state by means of combined use of titanium dioxide and the laccase, and by means of cooperation with other additives such as the plasticizer, the surfactant and the like, the environment-friendly and durable sisal bag is easy to degrade and has higher environmental resistance, water resistance, durability and antibacterial property.