47results about How to "Increase total saponin content" patented technology

The invention discloses a method for conducting wild tending of Chinese Paris rhizome under trees. The method is based on the planting principle of in-situ conversion, nearby development, wild tending and guaranteeing quality. For wild tending of Chinese Paris rhizome, a special traditional Chinese medicinal material cultivation medium suitable for growth of Chinese Paris rhizome and disease control is selected and applied, and a cultivation method comprising the steps of soil condition improving, land selection and preparation, reproduction, field management, disease and pest control, topping and harvesting is established. When the method for conducting wild tending of Chinese Paris rhizome under trees is used for cultivation of Chinese Paris rhizome, growth of roots and stems of Chinese Paris rhizome can be promoted, the incidence of diseases is reduced, the quality of the roots and the stems is improved, and the harvest yield is increased; the method can be used as a standard cultivation method for wild tending of Chinese Paris rhizome and has good development prospect.

The present invention relates to a method for mass propagating the adventitious root of ginseng, camphor ginseng, wild ginseng by tissue culture and improving the saponin content. More specially, the method comprises the steps of: tissue culturing of the leaf, root, stems of ginseng, camphor ginseng, wild ginseng and dissecting the callus; Propagating the adventitious root, which was developed by above dissected callus; Mass culturing of the propagated adventitious root in a bioreactor. Especially, the present invention makes it possible to produce the adventitious root that has enriched saponin content and the ratio of diol saponin and triol saponin being the same level of the natural ginseng, thereby, it can provide more valuable adventitious root in commercial and usefulness.

The invention relates to the field of foods, and particularly relates to noni puree and a preparation of the noni puree. The noni puree is prepared from the following raw materials: noni juice, noni pulp, hawthorn, white granulated sugar and anhydrous citric acid, wherein the preparation method of the noni juice comprises the following steps of: taking noni fruits, fermenting till the content of the soluble solids is smaller than 5.0%, removing the juice, and thus obtaining the noni juice. The preparation method of the noni pulp comprises the following steps of: taking noni fruits, fermenting till the content of the soluble solids is smaller than 5.0%, removing juice, crushing, filtering to remove crude fibers and seeds, thus obtaining the noni pulp. According to the invention, the noni fruits are sufficiently utilized, and after the hawthorn, white granulated sugar and anhydrous citric acid are added into the juice and the pulp obtained through fermentation, the noni puree with excellent mouthfeel is prepared, the noni fruits are sufficiently utilized, and waste of resources is avoided. The preparation method has simple technology and high yield, and the recovery rate can be as high as 50%.

The invention discloses a composition of probiotic-fermented corn stigma as well as a preparation method of the composition. The composition is prepared by the following steps: drying and crushing the corn stigma, soaking the corn stigma, conducting probiotic fermentation, and adding a sweetening agent. The corn stigma, through the probiotic fermentation, is relatively high in content of corn stigma total saponins and relatively low in molecular weight of corn stigma polysaccharides; and the fermented corn stigma can take such effects on lowering blood glucose, reducing blood fat, relieving swelling and inducing diuresis, clearing away heat and toxic materials, calming liver and benefiting gallbladders, improving constipation and the like. The composition of the probiotic-fermented corn stigma disclosed by the invention has easily acceptable taste and the composition is non-irritant to gastrointestinal tracts; and meanwhile, the composition, which contains probiotics and metabolites thereof, is capable of conditioning the environment of the gastrointestinal tracts and enhancing drug absorption.

A sealwort planting method sequentially comprises the following steps of sealwort rapid propagation and efficient sealwort cultivation. Sealwort rapid propagation can be sealwort rhizoma in-vitro rapid propagation and can also be sealwort seed rapid propagation. Efficient sealwort cultivation includes the substeps of land selection and soil preparation, planting, field management, disease and insect pest prevention, harvesting, processing and the like. The sealwort planting area can be enlarged, and the sealwort planting benefits are improved.

The invention discloses a polygonatum sibiricum planting method and belongs to the field of planting. The polygonatum sibiricum planting method includes land preparation, breeding, field management, pest control, harvest and primary processing and seed reservation. According to the polygonatum sibiricum planting method, polygonatum sibiricum and gramineae upright crops are interplanted. The polygonatum sibiricum planting method has the advantages that the polygonatum sibiricum planting method is capable of improving disease resistance of polygonatum sibiricum plants and increasing survival rate of polygonatum sibiricum seedlings, provides standard operation instructions for polygonatum sibiricum cultivation methods, fertilizer application and pest control, is beneficial to increase of annual output per mu and supplies high-quality, safe and stable medicinal herb resources for production of polygonatum sibiricum pharmaceuticals.

The invention relates to a Stauntonia brachyanthera Hand-Mazz component as well as a preparation method and application thereof, belonging to the technical field of medicines. The method comprises the following steps of: extracting Stauntonia brachyanthera Hand-Mazz by using water or organic solvents with different concentrations, filtering and recovering the solvents to obtain a concentrated solution; eluting the concentrated solution by using water and an organic solvent through macroporous absorption resin, recovering the solvent to obtain a saponin, or obtaining the saponin from an n-butyl alcohol layer by using an extracting method; then further separating and purifying the saponin by using a normal-phase silicagel column, a reversed phase column and a high efficient liquid phase preparation system; and finally obtaining a monomer or compound with remarkable activity. The Stauntonia brachyanthera Hand-Mazz component of the invention can be singly or combined with other drugs to be used as a raw drug applied to a drug for resisting tumour, resisting inflammation, promoting urination, and treating other diseases. The Stauntonia brachyanthera Hand-Mazz component prepared with the method has high purity, remarkable pharmacological action, simple and convenient method, practicability, low price, safety, suitability for industrialized production.

A ginseng biological composite bacterial manure consists of organic fertilizer 26-39wt%, turfy soil powder 2.4-3.6wt%, phosphorus rock powder 2-3wt%, calcium-magnesium phosphate phosphorus rock powder 2-3% proportion, lime hydrate powder 0.3-0.5%, mountain useful bacterial soil 8-13.4wt%, EM proliferous liquid 1.3-3.2wt%, hard-wood powder 6.4-9.6wt%, plant ash 0.4-0.6wt% and water 32-48wt%. It is convenient and cheap, has various material resources, it can degrade residual pesticide and other harmful substances and inhibit diseases effectively.

The invention relates to a use of yuenkanin total saponins extracted from yuenkanin plants for preparing weight-losing and blood lipid-lowering medicine, particularly losing weight, and simultaneously, the contents of TC, TG and LDL-C in blood serum can be further reduced and the content of HDL-C level is increased.

The invention belongs to the technical field of traditional Chinese medicines and relates to Radix Astragali extract and its preparation method and preparation. The Radix Astragali extract is used for treating shortness of breath, palpitaition, collapse, spontaneous perspiration, asthenia and edema, symptoms after tumor chemoradiotherapy and operation, chronic nephrosis, chronic diarrhea, proctoptosia, uterine prolapsed, gynecological diseases, ulcer fester and nonunion wounds. The Radix Astragali extract has obviously improved content of total saponins and astragaloside. The preparation method comprises the following steps of adding water into Radix Astragali, carrying out decoction, filtering the decoction, concentrating the filtrate, adding ethanol into the concentrated filtrate, carrying out standing, collecting a supernatant, recovering the ethanol, carrying out condensation and drying, and carrying out crushing to obtain fine powder. Aiming at solving defects of the existing preparation and satisfying clinical medicine requirements, the inventor carries out detailed research. The Radix Astragali extract as an active component and appropriate medicinal auxiliary materials are processed into the preparation by the conventional processes.

The invention provides a radix notoginseng extract which is extracted from fresh radix notoginseng as a raw material, and a preparation method of the radix notoginseng extract and a medicinal prescription prepared from the radix notoginseng extract as an active component. The radix notoginseng extract of the invention comprises the following components: 4-10%radix notoginseng saponin R1, 23-36% ginseng saponin Rg1, 2-5% ginseng saponin Re, 27-39% ginseng saponin Rb1 and 3-10% ginseng saponin Rd, wherein the radix notoginseng saponin R1, the ginseng saponin Rg1, the ginseng saponin Re, the ginseng saponin Rb1 and the ginseng saponin Rd account for 65-95% of the total weight. The radix notoginseng extract prepared by the method in the invention has few impurities, and total saponin component is high in impurity. The medicinal prescription prepared by the radix notoginseng extract as the active component has good curative effect and high safety.

The invention relates to a method for purifying panax notoginseng total saponins and a preparation of the panax notoginseng total saponins, and aims to provide a purifying method capable of preparing panax notoginseng total saponins with higher efficiency and higher purity and overcoming the defects of the prior art. The purifying method breaks through the conventional method for purifying the panax notoginseng total saponins; impurity removal, decolorization and enrichment of the total saponins can be effectively implemented by fully combining different purifying effects of macroporous resin, polyamide and active carbon; and the polyamide is filtered by one step of washing operation, so the process is simple, an organic solvent is saved and industrialized production is easily realized. The invention also comprises the medicinal preparation of the panax notoginseng total saponins, and the preparation can be prepared into power injection, injection, tablets or capsules.

The invention belongs to the technical field of food processing, and in particular relates to a Chinese yam polysaccharide, a Chinese yam healthcare wine and a production method thereof, aiming to solve a first technical problem of providing a Chinese yam polysaccharide and a preparation method thereof. As the Chinese yam polysaccharide is extracted by using alpha-amylase and saccharifying enzyme in an assistant manner, mere water extraction is avoided, the problem that the Chinese yam polysaccharide cannot be completely released as starch granule wrapping constraint is not broken can be solved, through enzymolysis, starch granules can be broken, dissolution of the Chinese yam polysaccharide can be promoted, and thus the yield of the Chinese yam polysaccharide is remarkably increased; and furthermore, starch mixed in an extract can be effectively eliminated, so that the purity of a crude polysaccharide in the obtained extract can be greatly increased. The Chinese yam healthcare wine prepared by using the method provided by the invention is light golden yellow in color, has wine fragrance and taste and flavor and smell of certain medicinal raw materials, is free of odor, and has very good market prospects.

The present invention relates to a method for mass propagating the adventitious root of ginseng, camphor ginseng, wild ginseng by tissue culture and improving the saponin content. More specially, the method comprises the steps of: tissue culturing of the leaf, root, stems of ginseng, camphor ginseng, wild ginseng and dissecting the callus; Propagating the adventitious root, which was developed by above dissected callus; Mass culturing of the propagated adventitious root in a bioreactor. Especially, the present invention makes it possible to produce the adventitious root that has enriched saponin content and the ratio of diol saponin and triol saponin being the same level of the natural ginseng, thereby, it can provide more valuable adventitious root in commercial and usefulness.

The invention discloses an efficient and stable asexual propagation method of panax notoginseng. The method comprises steps as follows: selection of explants; disinfection of the explants; induction of cluster buds; proliferation of the cluster buds; rooting culture; acclimatization and transplanting. The method is characterized in that a growth substrate for transplanting is prepared from following substances in parts by weight through mixing: 80-100 parts of garden soil, 25-35 parts of bentonite, 10-20 parts of kaolin, 10-20 parts of yellow sand, 3-7 parts of brown rice, 0.8-1.2 parts of water-soluble zinc salt, 0.8-1.2 parts of water-soluble magnesium salt, 0.6-0.8 parts of water-soluble potassium salt, 0.6-1.0 part of water-soluble ammonium salt and 4-8 parts of licorice extract. According to the asexual propagation method, the survival rate is high, obtained panax notoginseng seedlings have higher content of basic total saponins, and cultivation of the panax notoginseng with high content of total saponins is facilitated. With the adoption of the method, a large quantity of panax notoginseng seedlings can be obtained, the farmers' cost for obtaining the seedlings is reduced, and market competitiveness is improved.

The invention discloses an alfalfa squalene epoxidase gene overexpression method and application thereof. The alfalfa squalene epoxidase gene overexpression method comprises the following steps of (1)cloning and sequence analysis of alfalfa SE1 gene, (2) bioinformatics analysis, (3) subcellular localization, (4) prokaryotic expression, (5) expression of alfalfa squalene epoxidase MsSE1, and (6) MsSE1 gene overexpression carrier establishment and alfalfa genetic transformation. By establishing a high-efficiency genetic transformation system to transfer alfalfa squalene epoxidase genes into alfalfa for overexpression, the alfalfa squalene epoxidase gene overexpression method can improve the content of total saponins in transgenic alfalfa and provide breeding materials for culturing high-saponin-content alfalfa.

The invention discloses a post-harvest drying method for American ginseng. The post-harvest drying method comprises the following steps: pre-treating, grading, performing ultraviolet radiation, performing air-drying, performing NO fumigation and drying. The product quality of the American ginseng processed by the method is improved while the American ginseng is dried, content of active ingredients in the American ginseng can be greatly increased, color and luster, taste and nutrient ingredients of fresh American ginseng are completely kept, uniform water separation speed and a sufficient drying effect are achieved, and loss, caused by high-temperature heating, of various active ingredients is prevented; the content of the active ingredients of the dried American ginseng is obviously increased, and the content of main components such as ginsenoside is far higher than that by an existing drying method; and moreover, the dried product is uniform in color and luster, is stable in quality, is high in bioavailability, is strong in activity, is high in quality, is low in water content, is good in stability and is long in guarantee period.

The invention discloses a method for promoting accumulation of panax notoginseng saponins in a radix notoginseng medicinal material and belongs to the technical field of cultivation of medicinal plants. According to the method, nitrogen-potassium fertilizer with different proportions is applied to biennial or triennial radix notoginseng, and meanwhile, base fertilizer application or topdressing with different proportions is conducted; it is proved through experiments that according to a fertilization composition and an application mode, compared with a radix notoginseng medicinal material soldon the market, the content of all saponins in radix notoginseng main roots is increased by 10-15%; the method is simple, efficient and convenient to implement, fertilization is conducted while accumulation of panax notoginseng saponins is promoted, and the method is suitable for application and popularization in agricultural production.

The invention discloses a method for substantially increasing content of total saponins of panax notoginseng. Annual panax notoginseng with developed root systems and without diseases and damage is selected as a seedling and infiltrated with one or more of an ethanol solution of methyl jasmonate, a choline chloride water solution or a naphthylacetic acid solution before transplantation, plant probiotics are poured into soil surrounding the transplanted seedling 10-15 days after transplantation, a 20-30-fold diluted solution of an agrobacterium tumefaciens treatment agent or a 2-4-fold dilutedsolution of an agrobacterium tumefaciens resistant microbial agent is sprayed to the surrounding of the root part of a plant every two months after the seedling is transplanted for 1 year; after the seedling is transplanted for 2 years, a salicylic acid solution and a paclobutrazol water solution are uniformly sprayed to leaf surfaces every month on the basis of the spray amount allowing the leafsurfaces to be wet without liquid drops dripping; potassium fulvic acid is applied 5 months before harvesting. By means of the method, growth of main roots of the panax notoginseng can be promoted, the weight of the main roots is increased, the content of the total saponins of the panax notoginseng can be notably increased, the morbidity of root rot is reduced, the utilization rate of fertilizer is increased, and the cost is reduced. The method is easy to master, has remarkable benefits and is applicable to popularization.

The invention discloses a planting method for improving the platycodon grandiflorum yield and the medicine effect ingredient content, and belongs to the technical field of traditional Chinese medicinal material planting. According to the used method, DA-6 is sprayed for treatment; the combined treatment of the DA-6 spray application and the bud picking topping treatment is preferably selected. A method of combining DA-6 spray application and bud picking topping treatment is used, so that the single plant fresh weight and dry weight of platycodon grandiflorum can be improved by 50 percent or above; the diameter of platycodon grandiflorum roots can be increased by 45 percent or above; meanwhile, the lateral root number can be obviously reduced; the total platycodin content in the platycodongrandiflorum can also be increased by 56 percent or above; the platycodin-D content in the platycodon grandiflorum can be increased by 150 percent or above; the yield of the platycodon grandiflorum, particularly Zhongjiang platycodon grandiflorum, and the effective ingredient content in the medicinal material are obviously improved; the obvious economic benefits are achieved.

The invention discloses a growth matrix and a method for vegetative propagation of a medicinal plant paris polyphylla by employing the growth matrix. The growth matrix is prepared from the following ingredients in parts by weight: 120-160 parts of garden soil, 30-50 parts of high-calcium fly ash, 20-30 parts of laterite, 10-20 parts of attapulgite clay, 8-12 parts of sodium humate, 2-4 parts of water-soluble zinc salt, 1-3 parts of magnesium salt,1-3 parts of water-soluble sylvite, 1.5-2.5 parts of an water-soluble ammonium salt, 30-50 parts of water and 6-10 parts of a folium sennae extract. The preparation method of the folium sennae extract comprises the steps of carrying out heat reflux extraction on folium sennae by using an ethanol solution of which the volume percent is 70-90% for 2-4 times, mixing extract solutions, carrying out vacuum concentration and then carrying out spray drying to obtain the folium sennae extract. The growth matrix is stable and efficient, the survival rate is high, the basic total saponin content of seedlings is obviously improved, and growth of the medicinal paris polyphylla with abundant total saponin is facilitated.

The invention provides a preparation method of a radix ginseng rubra paste. The radix ginseng rubra paste is directly prepared from fresh ginseng or sun-dried ginseng, the step of preparing radix ginseng rubra first is avoided, the process is simple, cost is low, the energy consumption is low, the production period is shortened by 50% or above, and the preparation method is suitable for large-scale production. The heating time in the preparation process is short, the Maillard browning degree is low, and the product is good in color and luster, strong in flavor and good in water solubility. Extract extracted with alcohol is steamed, ginsenoside can be sufficiently converted, and water-soluble components in the extract are dissolved out and added to aqueous extract of ginseng. On the dry basis, the total saponin content of the radix ginseng rubra paste prepared from sun-dried ginseng is 3.2-3.4%, the total saponin content of the radix ginseng rubra paste prepared from fresh ginseng is 3.5-3.9%, and the total saponin content of an existing radix ginseng rubra paste is lower than 2.5%. The total saponin content of the radix ginseng rubra paste prepared from sun-dried ginseng is increased by 28-36%, and the total saponin content of the radix ginseng rubra paste prepared from fresh ginseng is increased by 40-56%. The content of ginsenoside monomer Rh1 is 0.17-0.45 mg / g, the content of Rh2 is 0.01-0.09 mg / g, the content of Rg3 is 0.56-1.60 mg / g, and the content of Rg5 is 0.41-0.95 mg / g.

The invention discloses a method for extracting total saponins from Mailuoning injection production waste liquid, which is characterized by comprising the following steps: (1) concentrating Mailuoninginjection production ester extraction waste liquid into an ester-flavor-free fluid extract, adding water, dissolving, and filtering to obtain a filtrate; (2) concentrating the filtrate under reducedpressure; (3) adsorbing with polyamide, washing with water to remove water-soluble impurities, carrying out gradient elution with ethanol, and collecting an eluent 1; (4) concentrating the alcohol eluent 1 under reduced pressure; (5) adsorbing by using macroporous resin, washing off water-soluble impurities by using water, carrying out gradient elution by using ethanol, and collecting an eluent 2;(6) concentrating the alcohol eluent 2 under reduced pressure until a crude extract of total saponins is obtained; and (7) dissolving the obtained crude extract of total saponins in a small amount ofabsolute methanol, then dropwise adding ethyl acetate, uniformly stirring until the total saponins are separated out, repeatedly treating for 5 times, collecting the separated total saponins, and finally concentrating under reduced pressure to obtain the extract of total saponins.

The invention discloses a composition for radix ophiopogonis planting. The composition is prepared from the following components in parts by weight: 799 to 1,051 parts of a nitrogen fertilizer, 1,904to 2,296 parts of a phosphate fertilizer, 823 to 901 parts of a potash fertilizer and 44 to 59 parts of paclobutrazol. The invention further provides the application of the composition in a Sichuan radix ophiopogonis planting process and an application method of the composition. According to the composition provided by the invention, on the basis of reducing the use amount of the paclobutrazol, the over-ground part of Sichuan radix ophiopogonis can fully grow; the leaf dry weight and the number of leaves are correspondingly increased, so that the yield and the quality of the Sichuan radix ophiopogonis are improved. Compared with the prior art, the composition disclosed by the invention has the advantages that the fresh yield is increased by 66.40 percent, the dry yield is increased by 66.46 percent, the content of water-soluble extracts is increased by 1.21 percent, and the content of total saponins is increased by 31.43 percent.

The invention discloses a method for cultivating organic ginsengs. The method comprises the steps of seed pretreatment, sowing and three-time transplanting. Seeds are transplant wild ginseng seeds. According to the step of sowing, rotten organic fertilizer for the ginseng bed is used as base fertilizer and is mixed evenly with soil, and then sowing is carried out. The three-time transplanting time respectively comprises four years after planting, eight years after planting and 12 years after planting. According to the method, the health growth of the ginsengs can be ensured, the growth period reaches more than 15 years, the disease resistance of the ginsengs is improved, the external manual plant protection effect of the ginsengs is reduced, the various effective components of the harvested ginsengs are obviously improved, and the quality and the yield of the ginsengs are greatly improved.

The invention discloses the brewing method for pseudo-ginseng beer, comprising the following steps: carrying out gelatinization, boiling, filtering, and getting wort, adding pseudo-ginseng alcohol extractive containing saponin into wort, sterilizing, cooling, and fermenting. The ratio of saponin and wort is 200-1000mg / L. The method keeps the beer vinous flavour and color and luster, and has the Notoginsen triterpenes function.

The invention provides red ginseng whole powder prepared through a composite enzymolysis method assisted by an extrusion technology and a preparation method. Red ginseng is pretreated through the extrusion technology, the red ginseng is subjected to enzymolysis through the composite enzymolysis method, and the prepared red ginseng whole powder is very high in total saponin content, soluble dietary fiber content and protein content; the nutritional value is more comprehensive and richer, and small molecules after enzymolysis are easier to absorb by a human body; the product is good in solubility, faint yellow in color and luster, free of earthy smell and good in sensory value; and the comprehensive utilization of ginseng products with high added value is realized, the obtained red ginseng whole powder can be used as a food base material, and a new method is provided for the development of ginseng food.

The invention discloses a method for greatly increasing the total saponin content of Panax notoginseng. Annual Panax notoginseng with well-developed root system, no disease and no damage is selected as seedlings, and ethanol solution of methyl jasmonate, choline chloride aqueous solution or One or more infiltration treatments in naphthalene acetic acid solution, 10-15 days after transplanting, pour plant probiotics into the soil around the transplanted seedlings, and after transplanting for 1 year, spread them on the roots of the plants every 2 months Spray Genailing 20-30 times solution or anti-rhizobacillus agent 2-4 times solution around; after transplanting for 2 years, evenly spray salicylic acid solution and paclobutrazol aqueous solution to the leaves every other month, spray The amount is based on the moistness of the leaf surface and no dripping; 5 months before harvest, apply potassium fulvic acid. The method of the invention can promote the growth of the main root of the notoginseng, increase the weight of the main root, significantly increase the content of the total saponins of the notoginseng, reduce the incidence of root rot, improve the utilization rate of the fertilizer, and reduce the cost. The method of the invention is easy to grasp, has obvious benefits, and is suitable for popularization.