Method for promoting accumulation of panax notoginseng saponins in radix notoginseng medicinal material

The technology of total saponins of Panax notoginseng and Panax notoginseng is applied in the directions of fertilization method, root crop cultivation, fertilization device, etc., can solve the problems of quality reduction of Panax notoginseng and the like, and achieves the effect of simple method, convenient implementation and improvement of total saponin content

Active Publication Date: 2019-02-19
KUNMING UNIV OF SCI & TECH
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  • Abstract
  • Description
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Problems solved by technology

Therefore, insufficient nitrogen fertilizer application or excessive nitrogen application will further inhibit the synthesis of notoginseng saponins, resulting in a decrease in the quality of notoginseng

Method used

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  • Method for promoting accumulation of panax notoginseng saponins in radix notoginseng medicinal material
  • Method for promoting accumulation of panax notoginseng saponins in radix notoginseng medicinal material
  • Method for promoting accumulation of panax notoginseng saponins in radix notoginseng medicinal material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: The method for promoting the accumulation of total saponins in Panax notoginseng medicinal materials is as follows:

[0014] During the first year of growth after transplanting of 1-year-old Panax notoginseng, the ratio of nitrogen fertilizer to potassium fertilizer is 1:2 by weight, and the application rate of nitrogen fertilizer (calculated as N) is 200 kg·hm -2 , nitrogen and potassium fertilizers (in K 2 (2) is 30% of base application, 70% of topdressing, and the time and amount of topdressing are 20% of topdressing in May, 10% of topdressing in June, 20% of topdressing in August and 20% of topdressing in October;

[0015] During the second year of growth after transplanting the 1-year-old Panax notoginseng, the mass ratios of nitrogen and potassium fertilizers in the three experimental fields were 1:1.5, 1:2 and 1:3, respectively, and the amount of nitrogen fertilizer (calculated as N) was 225 kg·hm -2 , potassium fertilizer (in K 2 O meter) dosage...

Embodiment 2

[0016] Embodiment 2: adopt the Panax notoginseng after the processing of embodiment 1 to measure the total saponin content in the main root

[0017] The root of Panax notoginseng was excavated after the seedlings fell, and the content of saponin in the notch, main root and tendon was measured; it was found that the total saponin content in the notch, main root and tendon of notoginseng was 16.84% under the condition of nitrogen and potassium ratio of 1:1.5 , 13.33% and 10.99%, which are 10.21%, 10.07% and 10.12% higher than those of commercially available medicinal materials; the total saponin content in notoginseng cuts, main roots and tendons were 17.12%, 13.65% under the condition of nitrogen and potassium ratio of 1:2 % and 11.29%, which are 12.04%, 12.72% and 13.13% higher than those of commercially available medicinal materials; it was found that the saponin content in notoginseng cuts, main roots and tendons were 17.57%, 13.93% and 13.13% respectively under the condition...

Embodiment 3

[0018] Example 3: Using the Panax notoginseng treated in Example 1 to determine the relative expression level of genes related to saponin synthesis in the main root

[0019] Before Radix Notoginseng harvesting, excavate Radix Notoginseng root, measure the relative expression of saponin synthesis-related genes in the main root of Radix Notoginseng after the treatment of Example 1, and simultaneously use Radix Notoginseng of customary fertilization as a contrast; find that the ratio of nitrogen and potassium is 1:1.5, Genes related to the synthetic pathway of Panax notoginseng taproot saponin Mevalonate under the conditions of 1:2 and 1:3 ACAT, HMAS, HMCAR, MVK, PMK, MDD, IDI , MEP pathway-related genes DXPS, DXPR, ispD, ispE, ispF, ispG, HDR and GGPS, FPS, SS, SE, DS, CAS The relative expression levels were significantly higher than those of customary fertilization ( figure 2 ); indicating that this fertilization method significantly up-regulated the expression of genes r...

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Abstract

The invention discloses a method for promoting accumulation of panax notoginseng saponins in a radix notoginseng medicinal material and belongs to the technical field of cultivation of medicinal plants. According to the method, nitrogen-potassium fertilizer with different proportions is applied to biennial or triennial radix notoginseng, and meanwhile, base fertilizer application or topdressing with different proportions is conducted; it is proved through experiments that according to a fertilization composition and an application mode, compared with a radix notoginseng medicinal material soldon the market, the content of all saponins in radix notoginseng main roots is increased by 10-15%; the method is simple, efficient and convenient to implement, fertilization is conducted while accumulation of panax notoginseng saponins is promoted, and the method is suitable for application and popularization in agricultural production.

Description

technical field [0001] The invention discloses a method for promoting the accumulation of total saponins of notoginseng in medicinal materials of notoginseng, belonging to the technical field of medicinal plant cultivation. Background technique [0002] Notoginseng Panax notoginseng (Bruk) F. H. Chen is a perennial herbaceous plant of the Araliaceae ginseng genus. Its dried roots are used as medicine. It has two-way regulating functions of hemostasis and blood circulation. Saponin is the main medicinal ingredient and quality evaluation index of Panax notoginseng, which is a triterpenoid secondary metabolite based on the carbon frame structure. According to the hypotheses of "carbon / nitrogen balance" and "growth / differentiation balance", plants will allocate more carbohydrates for their own growth when they grow in a nutrient-rich environment, but the limitation of nutrient deficiency in the growth environment is greater than that. During photosynthesis, plants allocate mor...

Claims

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Application Information

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IPC IPC(8): A01G22/25A01C21/00
CPCA01C21/005A01G22/25
Inventor 杨野石玥崔秀明刘迪秋曲媛王承潇杨晓艳
Owner KUNMING UNIV OF SCI & TECH
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