Method for extracting total saponins from Mailuoning injection production waste liquid

A technology of injection and total saponins, which is applied in the field of medicinal natural products, can solve the problems of no enterprise, low added value, and bring economic benefits, and achieve the effects of saving resources, low production costs, and protecting the environment

Inactive Publication Date: 2020-04-21
JINLING PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the waste residues of medicinal materials were transferred to fertilizer and feed companies to be processed into various bio-organic fertilizers, feed additives, microbial culture media, etc. However, due to the low added value, the transfer of waste residues of medicinal materials did not bring economic benefits to the enterprise

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Concentrate the waste liquid from the production of Mailuoning injection to 10 L of ester-free liquid extract, add 80 L of water to dissolve, filter, remove the water-insoluble matter, and obtain the filtrate; then concentrate the filtrate under reduced pressure (instrument: BÜCHI Rotary evaporator R-210, water bath 55°C, Vacuum Pump V-700 decompression pump, 70mbar (7 kPa)), concentrated under reduced pressure to about 1 / 3 volume of 30 L; adsorbed by 45 L polyamide (PA6), up The volume ratio of the sample volume to the polyamide was 2:3, and the flow rate was 8 mL / min. Then, the water-soluble impurities were washed away with water, and then the concentrations of 10%, 30%, 50%, 70%, and 95% ethanol were used successively. Carry out gradient elution, collect 10% and 30% alcohol eluents; concentrate 10% and 30% alcohol eluents under reduced pressure (instrument: BÜCHI rotary evaporator R-210, water bath 65°C, VacuumPump V-700 Decompression pump, 70 mbar (7 kPa)), concentr...

Embodiment 2

[0023]Concentrate the waste liquid from the production of Mailuoning injection to 10 L of ester-free fluid extract, add 100 L of water to dissolve, filter, remove the water-insoluble matter, and obtain the filtrate; then concentrate the filtrate under reduced pressure (instrument: BÜCHI Rotary evaporator R-210, water bath 60°C, Vacuum Pump V-700 decompression pump, 85 mbar (8.5kPa)), concentrated under reduced pressure to about 1 / 4 volume of 28 L; adsorbed by 56 L polyamide (PA66), The volume ratio of sample loading to polyamide was 1:2, and the flow rate was 10 mL / min. Then, the water-soluble impurities were washed away with water, and then the concentrations of 10%, 30%, 50%, 70%, and 95% were used successively. Carry out gradient elution with ethanol, collect 10% and 30% alcohol eluents; concentrate 10% and 30% alcohol eluents under reduced pressure (instrument: BÜCHI rotary evaporator R-210, water bath 70°C, Vacuum Pump V -700 decompression pump, 85mbar (8.5kPa)), to 1 / 3 o...

Embodiment 3

[0025] Concentrate the waste liquid from the production of Mailuoning injection to 10 L of ester-free fluid extract, add 120 L of water to dissolve, filter, remove the water-insoluble matter, and obtain the filtrate; then concentrate the filtrate under reduced pressure (instrument: BÜCHI Rotary evaporator R-210, water bath 65°C, Vacuum Pump V-700 decompression pump, 100mbar (10kPa)), decompression concentration to about 1 / 5 volume 26 L; the concentrated solution is passed through a 52 L polyamide column (PA1212) Adsorption, so that the active ingredients in the liquid are adsorbed on the polyamide resin, the volume ratio of the sample volume to the polyamide is 1:2, the flow rate is 10 mL / min, the effluent is discarded, and the resin is washed with distilled water of 1 column volume Column, discard the effluent, then wash the resin column with 20% ethanol of 2 times the column volume, discard the effluent, continue to carry out gradient elution with 10%, 30%, 50%, 70%, 90% etha...

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PUM

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Abstract

The invention discloses a method for extracting total saponins from Mailuoning injection production waste liquid, which is characterized by comprising the following steps: (1) concentrating Mailuoninginjection production ester extraction waste liquid into an ester-flavor-free fluid extract, adding water, dissolving, and filtering to obtain a filtrate; (2) concentrating the filtrate under reducedpressure; (3) adsorbing with polyamide, washing with water to remove water-soluble impurities, carrying out gradient elution with ethanol, and collecting an eluent 1; (4) concentrating the alcohol eluent 1 under reduced pressure; (5) adsorbing by using macroporous resin, washing off water-soluble impurities by using water, carrying out gradient elution by using ethanol, and collecting an eluent 2;(6) concentrating the alcohol eluent 2 under reduced pressure until a crude extract of total saponins is obtained; and (7) dissolving the obtained crude extract of total saponins in a small amount ofabsolute methanol, then dropwise adding ethyl acetate, uniformly stirring until the total saponins are separated out, repeatedly treating for 5 times, collecting the separated total saponins, and finally concentrating under reduced pressure to obtain the extract of total saponins.

Description

technical field [0001] The invention relates to the field of medicinal natural products, in particular to a method for extracting total saponins from production waste liquid of Mailuoning injection. Background technique [0002] Traditional Chinese medicine is the treasure of our nation and the most practical and helpful spirit in Chinese culture. For thousands of years, traditional Chinese medicine has made great contributions to the multiplication and prosperity of the Chinese nation, and it still plays an important and irreplaceable role in the cause of people's health until today. As the disadvantages of western medicine and western medicine continue to appear, more and more people believe in and use traditional medicine and treatment methods. [0003] Compared with traditional dosage forms such as pills, powders, ointments, and pills, traditional Chinese medicine injections are a unique new type of traditional Chinese medicine dosage form. It is a good option for pati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8984A61P3/06A61P35/00A61P29/00A61P39/06
CPCA61K36/21A61K36/355A61K36/808A61K36/8984A61K2236/51A61K2236/55A61P3/06A61P29/00A61P35/00A61P39/06A61K2300/00
Inventor 李剑徐向阳张蕙夏云倪洁张庆晓崔凌云周红燕
Owner JINLING PHARMA
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