44results about How to "High extraction and separation efficiency" patented technology

The invention belongs to the field of medical technology and relates to a new method for preparing xanthoceraside in shinyleaf yellowhorns. The highly purified xanthoceraside can be prepared efficiently with the preparation method. The preparation method is easy and convenient to use and reliable and reduces redundant intermediate operation steps, thus, product loss is reduced, and the yield of the preparation method is obviously increased compared with that of a former method. Crude extract is directly processed with a silica gel column, eluted with the chloroform / dichloromethane-methyl alcohol eluent with the volume ratio of 10:1-7:1, the ethyl acetate-methyl alcohol eluent with the volume ratio of 10:1-5:1 or the acetone-methyl alcohol eluent with the volume ratio of 10:1-6:1, and then eluted with the chloroform / dichloromethane-methyl alcohol eluent with the volume ratio of 6:1-2:1, the ethyl acetate-methyl alcohol eluent with the volume ratio of 4:1-1:1 or the acetone-methyl alcohol eluent with the volume ratio of 5:1-3:1, solvent recovery, purification and recrystallization are performed, and finally the xanthoceraside is obtained.

The invention discloses a process for preparing astragalus root saponins comprising the following steps in sequence, charging dissolvent into astragalus root, adjusting the solution to alkalinity, carrying out extraction, obtaining extract, passing the extract through macroscopic alkaline anion exchange resin, flushing with alkaline water and pure water, discarding the flushing fluid, eluting with water-containing ethanol, and concentrating the eluent.

The invention discloses a method for preparing hyperin from Dogbane leaves, which comprises the following steps: firstly. determining the optimum extraction temperature, a separation material and the concentration of an eluting agent; secondly, regulating the pH value, and extracting with a solvent having high hyperin dissolving and extracting power, thus obtaining a liquid extract; thirdly, heating at 80-85 DEG C under reflux of ethanol or methanol, and extracting; and finally, standing and crystallizing at a preferable cooling temperature, thus effectively separating the hyperin and isoquercitrin which are two flavonoid glycoside components having similar properties. In the invention, the hyperin is further purified by preparing a liquid phase; and a moving phase is examined and determined by methodology, and is subjected to targeted and efficient separation and purification based on the absorption wavelength of the hyperin, thus obtaining the hyperin having purity up to 90-99%. Thehyperin conforms to the requirements for Class A active pharmaceutical ingredients.

The invention relates to a method for separating ginkgolic acid monomers in total ginkgolic acid extracts by high-speed countercurrent chromatography. In the technical solution, silver nitrate is added to a two-phase solvent, and silver ion complexation is introduced. The two-phase solvent system For n-heptane / ethyl acetate / methanol / acetic acid. The method of the invention can separate ginkgolic acid, ginkgolic acid, heptadecanadiene ginkgolic acid, hydrogenated gingkoic acid, heptadecanone ginkgolic acid, five high-purity ginkgolic acid monomer compounds at one time, and the preparation method is simple and convenient High efficiency, cost-effective, suitable for industrial production, has important economic significance.

The invention relates to an extracting method of polysaccharide, which is characterized by comprising the following steps of: firstly, taking low-concentration ethanol as a solvent to extract and separate out micromolecule components by adopting a decompressing inner ebullition; then taking water as a solvent to extract polysaccharide from materials to be extracted by adopting the decompressing inner ebullition. Since the extracting temperature is low and the extracting time is short, starch can not be pasted and the swelling of the materials is small, the diffusion of the micromolecules of the polysaccharide is not influenced, and impurities of an extracting solution are less and the filtration is easy. Compared with the traditional polysaccharide extracting and separating technology, the consumption level of the ethanol of the extracting method of polysaccharide is reduced by more than 1 time, the speed of the extracting process is increased by 10-20 times, and the yield and the purity of the polysaccharide are respectively increased by 10-50 percent and 10-40 percent.

The invention belongs to the field of protein extraction, and specifically discloses a method for extracting and separating IgM and IgG from blood plasma, adjusting the pH value of the cold glue-free plasma to 6.3-7.0, and adjusting the conductivity of the cold-free plasma to 9-12mS / cm, carry out anion exchange chromatography to obtain the first flow-through solution and the anion gel packing with IgM protein adsorbed; adjust the pH value of the first flow-through solution to 5.4-5.6, and adjust the conductance of the first flow-through solution The rate was 2.0-4.0 mS / cm, and the cation exchange chromatography was carried out to obtain the second flow-through liquid and the cationic gel filler with IgG protein adsorbed thereon. The solution of the present invention can solve the problem of low efficiency of separating IgM and IgG.

The invention discloses a method for extracting polyphenols from olive processing waste liquid, and belongs to the technical field of biological separation. The invention uses olive processing waste liquid as raw material, adopts ethanol precipitation technology and macroporous adsorption resin separation technology, obtains high-quality olive polyphenols, and at the same time obtains functional feed additives and olive protein-polysaccharide complexes, and realizes olive processing waste liquid The comprehensive development and utilization of olive polyphenols, and provide technical support for the production of olive polyphenols, has good economic and social effects.

The invention discloses a preparation method of saponins from medicinal herbs which comprises, adding medicinal herbs into solvent, regulating to alkality, extracting, loading on macroporous basic anion-exchange column, eluting with alkali solution and pure water, discarding the flushing liquor, eluting with aqueous ethanol to give eluant, and concentrating.The inventive extraction method has theadvantages of high extraction efficiency, high saponins content, good biological activity presevation, and easy industrialization.

The invention relates to a Stauntonia brachyanthera Hand-Mazz component as well as a preparation method and application thereof, belonging to the technical field of medicines. The method comprises the following steps of: extracting Stauntonia brachyanthera Hand-Mazz by using water or organic solvents with different concentrations, filtering and recovering the solvents to obtain a concentrated solution; eluting the concentrated solution by using water and an organic solvent through macroporous absorption resin, recovering the solvent to obtain a saponin, or obtaining the saponin from an n-butyl alcohol layer by using an extracting method; then further separating and purifying the saponin by using a normal-phase silicagel column, a reversed phase column and a high efficient liquid phase preparation system; and finally obtaining a monomer or compound with remarkable activity. The Stauntonia brachyanthera Hand-Mazz component of the invention can be singly or combined with other drugs to be used as a raw drug applied to a drug for resisting tumour, resisting inflammation, promoting urination, and treating other diseases. The Stauntonia brachyanthera Hand-Mazz component prepared with the method has high purity, remarkable pharmacological action, simple and convenient method, practicability, low price, safety, suitability for industrialized production.