44results about How to "High extraction and separation efficiency" patented technology

The invention relates to a field of separation and purification of proteins, in particular to a novel method for extracting and separating proteins from cake by using an ionic liquid and an enzyme process. The method for extracting and separating proteins from cake by using an ionic liquid and an enzyme process disclosed by the invention comprises the following steps of: (1) smashing and sieving cake serving as a raw material, and degreasing to obtain degreased cake powder; (2) performing enzymolysis on the degreased cake powder under the condition that the pH is 7-10, the temperature is 40-65 DEG C and the material to liquid ratio is 1:7-1:15 in a state that the total enzyme activity is 90-250 U.g<-1> for 1-5 hours, and centrifugally separating; (3) performing alkali-solution and acid-isolation on the obtained precipitate and hydrolysate, and performing freeze drying to obtain crude proteins; (4) adding the crude proteins obtained in the step (3) into an ionic liquid / salt dual-water-phase system, and separating and purifying proteins at the temperature of 40-70 DEG C and at the pH of 4-9; and (5) separating proteins from an ionic liquid phase obtained in the step (4) with a membrane separation method, recovering an ionic liquid, and performing freeze drying to obtain high-purity protein powder. The method is easy to operate, is environment-friendly, and is a novel environment-friendly, energy-saving and efficient protein separating and purifying method; and the protein purity is high, and the bioactivity is kept.

The invention discloses a method for preparing hyperin from Dogbane leaves, which comprises the following steps: firstly. determining the optimum extraction temperature, a separation material and the concentration of an eluting agent; secondly, regulating the pH value, and extracting with a solvent having high hyperin dissolving and extracting power, thus obtaining a liquid extract; thirdly, heating at 80-85 DEG C under reflux of ethanol or methanol, and extracting; and finally, standing and crystallizing at a preferable cooling temperature, thus effectively separating the hyperin and isoquercitrin which are two flavonoid glycoside components having similar properties. In the invention, the hyperin is further purified by preparing a liquid phase; and a moving phase is examined and determined by methodology, and is subjected to targeted and efficient separation and purification based on the absorption wavelength of the hyperin, thus obtaining the hyperin having purity up to 90-99%. Thehyperin conforms to the requirements for Class A active pharmaceutical ingredients.

The invention discloses a preparation method of total saponins fromPlatycodon grandiflorum which comprises in sequence adding Platycodon grandiflorum into solvent, regulating to alkality, extracting, loading on macroporous basic anion-exchange column, eluting with alkali solution and pure water, discarding the flushing liquor, eluting with aqueous ethanol to give eluant, and concentrating. The inventive preparation method of Platycodon grandiflorum total saponins has high extraction separation efficiency, high total saponins yield, high biological activity preservation, and easy industrialization production.

The invention discloses a preparation method of total saponins from Ophiopogon japonicus, which comprises in sequence: adding Ophiopogon japonicus into solvent, regulating to alkality, extracting, loading on macroporous basic anion-exchange column, eluting with alkali solution and pure water, discarding the flushing liquor, eluting with aqueous ethanol to give eluant, and concentrating. The inventive preparation method of total saponins of Ophiopogon japonicus has high extraction separation efficiency, high total saponins content, high biological activity, and easy industrialization production.

The invention relates to a Stauntonia brachyanthera Hand-Mazz component as well as a preparation method and application thereof, belonging to the technical field of medicines. The method comprises the following steps of: extracting Stauntonia brachyanthera Hand-Mazz by using water or organic solvents with different concentrations, filtering and recovering the solvents to obtain a concentrated solution; eluting the concentrated solution by using water and an organic solvent through macroporous absorption resin, recovering the solvent to obtain a saponin, or obtaining the saponin from an n-butyl alcohol layer by using an extracting method; then further separating and purifying the saponin by using a normal-phase silicagel column, a reversed phase column and a high efficient liquid phase preparation system; and finally obtaining a monomer or compound with remarkable activity. The Stauntonia brachyanthera Hand-Mazz component of the invention can be singly or combined with other drugs to be used as a raw drug applied to a drug for resisting tumour, resisting inflammation, promoting urination, and treating other diseases. The Stauntonia brachyanthera Hand-Mazz component prepared with the method has high purity, remarkable pharmacological action, simple and convenient method, practicability, low price, safety, suitability for industrialized production.

The invention relates to a preparation method of Jasminum lanceolarium medium-ring iridoids and application of the Jasminum lanceolarium medium-ring iridoids in inflammation resistance and tumor resistance, belonging to the technical field of medicine. The preparation method comprises the following steps: pulverizing dry Jasminum lanceolarium stem, carrying out flash extraction to obtain a total extracting solution, and carrying out macroporous resin column chromatography on the total extracting solution to obtain the crude extract containing rich iridoids; and separating by normal-phase silica gel column chromatography, screening the effective fraction containing rich iridoids, separating the effective fraction by semipreparative or preparative HPLC (high performance liquid chromatography), and eluting by using methanol-water, acetonitrile-water or methanol-acetonitrile-water as the mobile phase to obtain the iridoids. The preparation method provided by the invention is convenient and reliable, has the advantages of high extraction separation efficiency and strong operability, and can easily implement industrialized mass production; and the prepared iridoids have anti-inflammatory and antitumor activities.

The invention discloses a preparation method of saponins from medicinal herbs which comprises, adding medicinal herbs into solvent, regulating to alkality, extracting, loading on macroporous basic anion-exchange column, eluting with alkali solution and pure water, discarding the flushing liquor, eluting with aqueous ethanol to give eluant, and concentrating.The inventive extraction method has the advantages of high extraction efficiency, high saponins content, good biological activity presevation, and easy industrialization.

The invention discloses a process for preparing acanthopanax root saponin extract comprising the following steps in sequence, charging dissolvent into acanthopanax root, rhizome, stem or leaf, adjusting the solution to alkalinity, carrying out extraction, obtaining extract, passing the extract through macroscopic alkaline anion exchange resin, flushing with alkaline water and pure water, discarding the flushing fluid, eluting with water-containing ethanol, and concentrating the eluent.

The invention discloses a process for preparing ginseng saponin comprising the following steps in sequence, charging dissolvent into ginseng root, rhizome or ginseng leaves, adjusting the solution to alkalinity, carrying out extraction, obtaining extract, passing the extract through macroscopic alkaline anion exchange resin, flushing with alkaline water and pure water, discarding the flushing fluid, eluting with water-containing ethanol, and concentrating the eluent.

The invention discloses a method for preparing notoginsen triterpenes. It comprises following steps: (1) collecting notoginseng roots or leaves, feeding them into disslovant, moderating to basic with solution, extracting and getting extract; (2) feeding extract into large- porous basic anion exchange column, washing with basic solution and pure water, getting rid of washing liquid, washing with water- containing ethanol, condensing washing liquid, and getting final product. The invention is characterized by high extract separatio rate, high total concentration of notoginsen triterpenes, strong physiologically activity and suitability for industrial production.

The invention provides an extraction preparation method of triptolide. The method comprises the steps of extracting thunder god vine herbs by utilizing ethyl acetate, dissolving extract by utilizing dichloromethane, then carrying out the alkali wash, carrying out the secondary positive silica-gel column chromatography, carrying out the elution sequentially by utilizing dichloromethane, dichloromethane / methanol mixed solvent and methanol for the primary column chromatography, carrying out the gradient elution by utilizing petroleum ether / ethyl acetate mixed solvent for the secondary column chromatography, and finally preparing a triptolide pure product in a crystallizing manner. The preparation method is fewer in steps and simple to operate, the triptolide product with high purity can be effectively obtained, and the production cost of the triptolide can be greatly reduced.

The invention relates to an extracting method for medium and low temperature hemicellulose, and is capable of effectively improving the extraction separation efficiency of the hemicellulose, shortening the extraction time, saving cost, and improving the product quality and yield. The method comprises the following steps: cutting plant fibrous raw materials to be sections or slices, washing, air-drying, degreasing by using benzyl alcoholic solution, adding a degreasing raw material to alkali liquor, heating, executing ultrasound reaction, sieving by using 200 meshes of a sieve, concentrating the filter liquor by using an ultrafiltration membrane, adjusting pH value to be 5.5, adding ethyl alcohol, stewing in the room temperature, centrifuging, washing sediment by using the ethyl alcohol, and washing the sediment to be neutral by using deionized water, freezing and drying to obtain the hemicellulose. The method is scientific and rational, and easy to operate. The production efficiency is high, the using effect is good, and the operation is simple and easy. The yield and purity of extracting the hemicellulose can be improved, the extraction separating efficiency of the hemicellulose is effectively improved, the extraction time is shortened, the cost is saved, and the quality and yield of the product are improved. The extracting method is a big innovation for extracting the hemicellulose, and has remarkable economic and social benefits.

The invention provides a plant extract used for preparing medicine for treating asthma, which is a mixture formed by rhododendron mariae extract and Japanese ardisia extract according to any proportion, the mixture is an extract prepared after the rhododendron mariae is mixed with the Japanese ardisa or is prepared by the steps of preparing the rhododendron mariae extract and the Japanese ardisia extract and before mixing the two extracts. The invention also provides the application of the extract in the preparation of the medicine for treating asthma; the extract and pharmaceutically receivable minor ingredients are made into the medicine preparation, and the medicine preparation can be dripping pills, troche, capsule, granules or other common medicine preparations. The extract has less components, very simple preparation process, more scientific and reasonable prescription, strong specific treatment and remarkable treatment effect, uses the traditional famous prescription as support, and combines the traditional Chinese medicine theory with years of clinical experiences.

The invention discloses an extraction separation method of a fat-soluble metabolite in faeces. The extraction separation method comprises the following steps: extracting the metabolite in the faeces by an organic solvent, carrying out gradient chromatography by a petroleum ether / ethyl acetate elution system I and a dichloromethane / methyl alcohol elution system II, and separating metabolite monomers through high performance liquid chromatography. According to the extraction separation method, large-polarity and small-polarity impurities in a sample are removed through normal-phase silica gel column chromatography of gradient elution, the method plays a good role in enrichment and separation for the metabolite, the metabolite monomers can be obtained through extraction and separation, and the disadvantage that isomerides of the metabolite are difficultly identified by HPLC-MS is overcome; and moreover, while subsequent structure determination is guaranteed to be completed, an extracted and separated metabolite standard sample can be obtained.

The invention belongs to the field of medical technology and relates to a new method for preparing xanthoceraside in shinyleaf yellowhorns. The highly purified xanthoceraside can be prepared efficiently with the preparation method. The preparation method is easy and convenient to use and reliable and reduces redundant intermediate operation steps, thus, product loss is reduced, and the yield of the preparation method is obviously increased compared with that of a former method. Crude extract is directly processed with a silica gel column, eluted with the chloroform / dichloromethane-methyl alcohol eluent with the volume ratio of 10:1-7:1, the ethyl acetate-methyl alcohol eluent with the volume ratio of 10:1-5:1 or the acetone-methyl alcohol eluent with the volume ratio of 10:1-6:1, and then eluted with the chloroform / dichloromethane-methyl alcohol eluent with the volume ratio of 6:1-2:1, the ethyl acetate-methyl alcohol eluent with the volume ratio of 4:1-1:1 or the acetone-methyl alcohol eluent with the volume ratio of 5:1-3:1, solvent recovery, purification and recrystallization are performed, and finally the xanthoceraside is obtained.

The invention relates to a method for purifying ganoderic acid T and ganoderic acid Me based on a chemical synthesis method, belonging to the technical field of extraction of traditional Chinese medicines. The method comprises the following steps of: concentrating a ganoderic acid crude extract to extractum-shaped ganoderma cell extract extractum through rotary evaporator treatment, then dissolving the ganoderma cell extract extractum to an organic solvent and adding an acetylation reagent, carrying out acetylation reaction under the monitoring of reversed phase liquid chromatography, extracting the acetylation reagent by utilizing a neutralizer, concentrating ganoderma cell extract after acetylation to form an extractum shape through the rotary evaporator treatment, renewedly dissolving the ganoderma cell extract to a mixed system of carbinol or carbinol and water, adding an acid catalyst for carrying out acid catalyzed reaction under the monitoring of the reversed phase liquid chromatography, and finally placing neutralized acid catalytic reaction products in a rotary evaporator for volatilizing to dryness to obtain ganoderic acid crude products with more than 45% of ganoderic acid T and more than 25% of ganoderic acid Me.

The invention discloses a method for treating an organic silicon low-boiling-point substance by utilizing reactive distillation. The method comprises the following steps: (1) treating a filler layer of a reaction section; (2) mounting a filler layer; (3) rectification treatment: feeding the organic silicon low-boiling-point substance, methyl trichlorosilane, trimethylchlorosilane and a cocatalyst methyl hydrogen dichlorosilane into the surface of a solid catalytic filler layer in a reaction section in a rectification tower to perform disproportionation reaction, and enabling dimethyl dichlorosilane generated in the disproportionation reaction and mixed gas which is not completely reacted to flow downwards to form mixed liquid, wherein one part of the mixed liquid is extracted through a tower kettle discharge pipe and enters a rectification raw material unit for separation treatment, the other part of the mixed liquid is extracted through a tower kettle bypass pipe and is heated by a reboiler to form steam, the steam enters a rectification tower, and low-boiling-point gas which is not completely reacted in the disproportionation reaction and mainly comprises trimethylchlorosilane is extracted through a tower top discharge pipe and conveyed to a condenser. The method has the advantages of being short in process, high in product yield, low in operation cost and capable of achieving continuous and efficient operation.

The invention belongs to the field of protein extraction, and specifically discloses a method for extracting and separating IgM and IgG from plasma. The pH value of cold-glue removed plasma is adjusted to 6.3-7.0, and the conductivity of cold-glue removed plasma is adjusted to 9-12 mS / cm, anion exchange chromatography is carried out to obtain a first flow-through liquid and an anionic gel packingwith IgM protein adsorbed; and the pH value of the first flow-through liquid is adjusted to 5.4-5.6, the conductivity of the first flow-through fluid is adjusted to 2.0-4.0 mS / cm, and cation exchangechromatography is performed to obtain a second flow-through fluid and a cation gel packing with IgG protein adsorbed. By adopting the scheme of the invention, the problem of lower separation efficiency of IgM and IgG can be solved.

The invention discloses a method for extracting and separating six dihydrobenzophenanthridine alkaloids from macleaya cordata. The method comprises the following steps: carrying out solvent extraction, concentration, separation and purification on macleaya cordata raw materials to obtain 6-(2'-ethyl formate)dihydrosanguinarine, 6-(2'-ethyl carboxylate)dihydrochelerythrine, 6-methyl carboxylate dihydrosanguinarine, 6-ethyl carboxylate dihydrosanguinarine, 6-(2'-ethyl carboxylate)dihydrosanguinarine and 6-ethyl isocarboxylate dihydrosanguinarine. According to the method, the six dihydrotriphenanthridine alkaloids are successfully extracted and separated from macleaya cordata, the extraction and separation efficiency is high, and the purity of the obtained alkaloids is high. According to themethod, the resource utilization of macleaya cordata is improved, and an effective way is provided for cognitive research and extraction separation of active ingredients of new drugs.

The invention discloses an oil-water separation device for extracting plant essential oil. The oil-water separation device comprises a separation device body, the outer side of the separation device body is connected with a rack, one side, far away from the rack, of the separation device body is connected with a steam condensation tank, a floating extraction mechanism is arranged in the separation device body, and the floating extraction mechanism comprises a supporting floating plate; a balance weight ring is connected to the outer side of the supporting floating plate, a suction nozzle fixing plate is arranged in the supporting floating plate, and a plurality of evenly-distributed extraction suction nozzles are connected to the side, away from the supporting floating plate, of the suction nozzle fixing plate. The corresponding floating type extraction mechanism is additionally arranged on the oil-water separation device, so that the effect of extracting and separating the plant essential oil in the oil-water separation device is remarkably improved, the situation that the plant essential oil in the oil-water separation device is not thoroughly separated is avoided, waste of the plant essential oil is reduced, the extraction and separation efficiency of the plant essential oil in the oil-water separation device is greatly improved, and the production cost of the plant essential oil is reduced.

The invention discloses a method for preparing hyperin from Dogbane leaves, which comprises the following steps: firstly. determining the optimum extraction temperature, a separation material and the concentration of an eluting agent; secondly, regulating the pH value, and extracting with a solvent having high hyperin dissolving and extracting power, thus obtaining a liquid extract; thirdly, heating at 80-85 DEG C under reflux of ethanol or methanol, and extracting; and finally, standing and crystallizing at a preferable cooling temperature, thus effectively separating the hyperin and isoquercitrin which are two flavonoid glycoside components having similar properties. In the invention, the hyperin is further purified by preparing a liquid phase; and a moving phase is examined and determined by methodology, and is subjected to targeted and efficient separation and purification based on the absorption wavelength of the hyperin, thus obtaining the hyperin having purity up to 90-99%. Thehyperin conforms to the requirements for Class A active pharmaceutical ingredients.

The invention discloses a sample extraction and separation device for corn flour biotoxin detection. The sample extraction and separation device comprises a separation box and a box cover located on one side of the top of the separation box. According to the sample extraction and separation device for corn flour biotoxin detection, when corn flour is fed, grinding treatment is conducted on the corn flour in advance, it is guaranteed that the thickness of the corn flour entering the centrifugal tube is consistent, and the sample extraction and separation effect is improved. Through the arranged extraction mechanism, centrifugal force is applied to conduct centrifugal treatment on the mixture in each centrifugal tube until the interior of each centrifugal tube is layered up and down, a driving motor is turned off, an output shaft of the driving motor drives a centrifugal frame to rotate until the multiple centrifugal tubes are located under the multiple liquid guide tubes, and finally supernate in the multiple centrifugal tubes is extracted through a liquid extraction tube, so a virus detection sample is obtained. The extraction and separation efficiency of the sample is effectively improved, and the phenomenon of sample emulsification is avoided.

The invention discloses a method for extracting polyphenols from olive processing waste liquid, and belongs to the technical field of biological separation. The invention uses olive processing waste liquid as raw material, adopts ethanol precipitation technology and macroporous adsorption resin separation technology, obtains high-quality olive polyphenols, and at the same time obtains functional feed additives and olive protein-polysaccharide complexes, and realizes olive processing waste liquid The comprehensive development and utilization of olive polyphenols, and provide technical support for the production of olive polyphenols, has good economic and social effects.

The invention discloses a method for extracting and separating six dihydrobenzophenanthridine alkaloids from macleaya cordata. The method comprises the following steps: carrying out solvent extraction, concentration, separation and purification on macleaya cordata raw materials to obtain 6-(2'-ethyl formate)dihydrosanguinarine, 6-(2'-ethyl carboxylate)dihydrochelerythrine, 6-methyl carboxylate dihydrosanguinarine, 6-ethyl carboxylate dihydrosanguinarine, 6-(2'-ethyl carboxylate)dihydrosanguinarine and 6-ethyl isocarboxylate dihydrosanguinarine. According to the method, the six dihydrotriphenanthridine alkaloids are successfully extracted and separated from macleaya cordata, the extraction and separation efficiency is high, and the purity of the obtained alkaloids is high. According to themethod, the resource utilization of macleaya cordata is improved, and an effective way is provided for cognitive research and extraction separation of active ingredients of new drugs.

The invention discloses a preparation method of saponins from medicinal herbs which comprises, adding medicinal herbs into solvent, regulating to alkality, extracting, loading on macroporous basic anion-exchange column, eluting with alkali solution and pure water, discarding the flushing liquor, eluting with aqueous ethanol to give eluant, and concentrating.The inventive extraction method has theadvantages of high extraction efficiency, high saponins content, good biological activity presevation, and easy industrialization.

The invention relates to a Stauntonia brachyanthera Hand-Mazz component as well as a preparation method and application thereof, belonging to the technical field of medicines. The method comprises the following steps of: extracting Stauntonia brachyanthera Hand-Mazz by using water or organic solvents with different concentrations, filtering and recovering the solvents to obtain a concentrated solution; eluting the concentrated solution by using water and an organic solvent through macroporous absorption resin, recovering the solvent to obtain a saponin, or obtaining the saponin from an n-butyl alcohol layer by using an extracting method; then further separating and purifying the saponin by using a normal-phase silicagel column, a reversed phase column and a high efficient liquid phase preparation system; and finally obtaining a monomer or compound with remarkable activity. The Stauntonia brachyanthera Hand-Mazz component of the invention can be singly or combined with other drugs to be used as a raw drug applied to a drug for resisting tumour, resisting inflammation, promoting urination, and treating other diseases. The Stauntonia brachyanthera Hand-Mazz component prepared with the method has high purity, remarkable pharmacological action, simple and convenient method, practicability, low price, safety, suitability for industrialized production.