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A kind of preparation method of ginkgolic acid

A technology of ginkgolic acid and total ginkgolic acid, which is applied in the separation/purification of carboxylic acid compounds, organic chemistry, etc., can solve the problems of complex components, lack of reference significance, and unclear content, and achieve simple operation, cost economy, high efficiency effect

Active Publication Date: 2021-04-27
SHANDONG ANALYSIS & TEST CENT
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  • Claims
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AI Technical Summary

Problems solved by technology

The extraction and separation process of traditional Chinese medicine, the compounds contained in it are complex and the content is not clear
Therefore, the exploration of the solvent system is a complex task, and the extraction methods in the prior art often have no reference significance

Method used

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  • A kind of preparation method of ginkgolic acid
  • A kind of preparation method of ginkgolic acid
  • A kind of preparation method of ginkgolic acid

Examples

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Embodiment 1

[0029] 1. Sample Extraction

[0030] Separate the testa of ginkgo fruit from the ginkgo fruit, place the testa in a cool place to dry, and extract 5 kg of the dried testa with 10 L of petroleum ether (boiling range 60-90 ° C) for 3 times, each time for 2 hours, and then The extract was filtered using a vacuum filter unit. All the extracts were combined and evaporated to dryness at 55° C. to obtain 247 g of total ginkgolic acid component extract, which was stored in a refrigerator at 4° C. for further HSCCC purification.

[0031] 2. Separation and purification of ginkgolic acid monomers in Ginkgo biloba exocarp by online collection and cyclic countercurrent chromatography

[0032] Prepare the two-phase solvent system n-heptane / ethyl acetate / methanol / acetic acid (5:4:1:1, v / v) according to the above solvent ratio, place it in a separatory funnel, and add 0.20 mmol / L of For the silver nitrate solution, shake it up and let it stand for stratification. After a period of equilibri...

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Abstract

The invention relates to a method for separating ginkgolic acid monomers in total ginkgolic acid extracts by high-speed countercurrent chromatography. In the technical solution, silver nitrate is added to a two-phase solvent, and silver ion complexation is introduced. The two-phase solvent system For n-heptane / ethyl acetate / methanol / acetic acid. The method of the invention can separate ginkgolic acid, ginkgolic acid, heptadecanadiene ginkgolic acid, hydrogenated gingkoic acid, heptadecanone ginkgolic acid, five high-purity ginkgolic acid monomer compounds at one time, and the preparation method is simple and convenient High efficiency, cost-effective, suitable for industrial production, has important economic significance.

Description

technical field [0001] The invention relates to a method for separating and purifying the active ingredients of traditional Chinese medicine, specifically, using countercurrent chromatography to separate ginkgolic acid monomer compounds (ginkgolic acid, ginkgolic acid, heptadecanadiene ginkgolic acid, hydrogenated ginkgolic acid, heptadecane- alkenyl ginkgolic acid) method. Background technique [0002] Ginkgolic acid compounds are another important medicinal ingredient besides flavonoids and lactones in the middle leaves, ginkgo and pericarp of Ginkgo biloba, which are derivatives of 6-alkenyl salicylic acid. Modern pharmacological studies have shown that ginkgolic acids have antiviral, antibacterial, anti-inflammatory, anti-cancer, anti-parasitic and other pharmacological activities. Since the ginkgolic acid component is a low-polarity structural analogue, it is difficult to separate. The traditional separation methods of monomer components include silica gel column chro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C51/42C07C65/05C07C65/19
CPCC07C51/42C07C65/05C07C65/19
Inventor 王岱杰闫慧娇耿岩玲赵恒强李丽丽朱姮
Owner SHANDONG ANALYSIS & TEST CENT
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