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Stauntonia brachyanthera Hand-Mazz saponins components as well as preparation method and application thereof

A technology of fructoside and yellow wax fruit, which is applied in the field of preparation of yellow wax fruit saponins, and achieves the effects of high content, high extraction and separation efficiency, and strong operability

Active Publication Date: 2014-04-23
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

) But so far, it is still a blank in the field of chemical research, this patent will be the first time to separate, analyze and study the chemical components of the plant

Method used

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  • Stauntonia brachyanthera Hand-Mazz saponins components as well as preparation method and application thereof
  • Stauntonia brachyanthera Hand-Mazz saponins components as well as preparation method and application thereof
  • Stauntonia brachyanthera Hand-Mazz saponins components as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1 kg of Huanglaguo root powder, soaked and extracted three times with 12 times, 10 times and 8 times of 70% alcohol at 80°C, (3 hours for the first time, 2 hours for the second time, 2 hours for the third time) and filtered , merge the filtrate, reclaim the solvent or evaporate to dryness until concentrated to 1 liter, according to the macroporous adsorption resin and medicinal material amount according to the macroporous adsorption resin (HPD100) column chromatography separation in the ratio of 1: 1, adopt the gradient elution method, successively water , 40% water-containing alcohol, 70% water-containing alcohol, and 90% water-containing ethanol for elution, wash 8 retention volumes for each gradient, combine ethanol eluents, decolorize with magnesium oxide, and add magnesium oxide to reflux at 80 grams per liter Decolorize for 1 hour, filter, and the filtrate is concentrated under reduced pressure and dried to obtain 170 g (yield 17%) of the total saponins of the frui...

Embodiment 2

[0026] 1 kg of yellow wax fruit stem powder, soaked and extracted three times with 10 times, 8 times and 6 times of 60% alcohol at 60°C, (3 hours for the first time, 2 hours for the second time, 2 hours for the third time) and filtered , combine the filtrate, reclaim the solvent or evaporate to dryness until concentrated to 1 liter, the above-mentioned total extract is extracted 3-8 times with 0.5-3 times the volume of water-saturated n-butanol according to the ratio of medicinal material: n-butanol as 1:1 , combined with n-butanol to obtain the n-butanol extract of Huanglaguo. Decolorize with magnesia, add 80 grams per liter of magnesia to reflux for decolorization for 1 hour, filter, and the filtrate is concentrated under reduced pressure and dried to obtain 182g (yield 18.2%) of the total saponins of huangcuruo. Saponins are further separated and purified by normal phase silica gel column chromatography, and eluted with different ratios of chloroform-methanol or acetone-met...

Embodiment 3

[0028] 1 kg of yellow wax fruit leaf powder, soaked and extracted three times with 8 times, 6 times and 6 times of 65% alcohol at 80°C, (3 hours for the first time, 2 hours for the second time, 2 hours for the third time) filter , merge the filtrate, reclaim the solvent or evaporate to dryness until concentrated to 1 liter, according to the macroporous adsorption resin and medicinal material amount according to the macroporous adsorption resin (HPD100) column chromatography separation in the ratio of 1: 1, adopt the gradient elution method, successively water , 40% water-containing alcohol, 70% water-containing alcohol, and 90% water-containing ethanol for elution, wash 9 retention volumes for each gradient, combine the ethanol eluents, decolorize with magnesium oxide, and add magnesium oxide to reflux at 80 grams per liter Decolorize for 1 hour, filter, and the filtrate is concentrated under reduced pressure and dried to obtain 120 g (yield 12%) of the total saponins of the fr...

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Abstract

The invention relates to a Stauntonia brachyanthera Hand-Mazz component as well as a preparation method and application thereof, belonging to the technical field of medicines. The method comprises the following steps of: extracting Stauntonia brachyanthera Hand-Mazz by using water or organic solvents with different concentrations, filtering and recovering the solvents to obtain a concentrated solution; eluting the concentrated solution by using water and an organic solvent through macroporous absorption resin, recovering the solvent to obtain a saponin, or obtaining the saponin from an n-butyl alcohol layer by using an extracting method; then further separating and purifying the saponin by using a normal-phase silicagel column, a reversed phase column and a high efficient liquid phase preparation system; and finally obtaining a monomer or compound with remarkable activity. The Stauntonia brachyanthera Hand-Mazz component of the invention can be singly or combined with other drugs to be used as a raw drug applied to a drug for resisting tumour, resisting inflammation, promoting urination, and treating other diseases. The Stauntonia brachyanthera Hand-Mazz component prepared with the method has high purity, remarkable pharmacological action, simple and convenient method, practicability, low price, safety, suitability for industrialized production.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a method for extracting and preparing medicinal raw materials and an application thereof, in particular to a method for preparing and application of saponin components of japonica. Background technique [0002] Huanglaguo (Stauntonia brachyanthera Hand-Mazz) is a plant of the genus Wild Papaya in the family Akebia. It is distributed in Guizhou, Guangxi and other provinces (regions) in my country. It is an evergreen woody vine. The effects of clearing away heat and diuresis, dredging meridian and activating collaterals, relieving pain and draining pus, clearing milk, etc. are the same as Akebia. The shape of the fruit is basically the same as that of Zhizhizi. Huangla fruit does not crack after ripening, and its color is yellow. Folks also use it as a medicine for predicting seeds; the peel contains many stone cells, and it is used by folks for stones. The leaves are also used as me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00C07J17/00A61K36/185A61K31/704A61K31/56A61P35/00A61P29/00A61P7/10
Inventor 孟大利陈超耿剑亮李铣李宁
Owner SHENYANG PHARMA UNIVERSITY
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