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Method for extracting olive polyphenol from olive processing waste liquor

A technology for olive polyphenols and processing waste liquid, which can be applied in pharmaceutical formulations, non-central analgesics, medical preparations containing active ingredients, etc., can solve problems such as environmental pollution and resource waste, and achieve high purity and extraction and separation efficiency. The effect of high and good potential for industrial application

Inactive Publication Date: 2015-02-18
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the process of producing and processing olive oil with olive fruit, a large amount of waste liquid is produced, which contains high concentrations of polyphenolic compounds, proteins, polysaccharides and vitamins and other biologically active components, but these waste liquids have not been developed and utilized, not only It caused a great waste of resources, and the direct discharge of olive processing waste liquid caused environmental pollution

Method used

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  • Method for extracting olive polyphenol from olive processing waste liquor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 1000 mL of fresh olive processing waste liquid (polyphenol content is 4.6 g / L), and filter it with 80 mesh filter cloth to obtain filter residue A and filtrate A. Filtrate A is centrifuged at 2000 rpm for 30 min to obtain supernatant B and precipitate B , mix filter residue A and precipitate B, dry and pulverize with hot air to obtain a functional feed additive.

[0021] Add absolute ethanol to the supernatant B so that the volume percentage of ethanol in the mixture is 20%, stir well and precipitate at 0°C for 2 h, and centrifuge the mixture at 2000 rpm for 30 min to obtain supernatant C and precipitate C; Precipitate C was washed with 50% ethanol solution until the washing liquid was colorless, washing liquid D and precipitate D were collected, and precipitate D was vacuum-dried or freeze-dried to obtain the olive polysaccharide-protein complex.

[0022] Combine supernatant C and washing solution D, recover ethanol after concentrating under vacuum and decompressi...

Embodiment 2

[0024] Take 800 mL of fresh olive processing waste liquid (polyphenol content is 4.2 g / L), and filter it with a 100-mesh filter cloth to obtain filter residue A and filtrate A. Filtrate A is centrifuged at 3000 rpm for 20 min to obtain supernatant B and precipitate B , mix filter residue A and precipitate B, dry and pulverize with hot air to obtain a functional feed additive.

[0025] Add absolute ethanol to the supernatant B to make the volume fraction of ethanol in the mixture 30%, stir well and precipitate at 5 °C for 4 h, and centrifuge the mixture at 3000 rpm for 20 min to obtain supernatant C and precipitate C, Precipitate C was washed with 60% ethanol aqueous solution until the washing liquid was colorless, washing liquid D and precipitate D were collected, and precipitate D was vacuum-dried or freeze-dried to obtain the olive polysaccharide-protein complex.

[0026] Combine the supernatant C and the washing solution D, concentrate and recover ethanol under vacuum to ob...

Embodiment 3

[0028] Take 600 mL of waste liquid from fresh olive processing (polyphenol content is 2.9 g / L), and filter it with a 150-mesh filter cloth to obtain filter residue A and filtrate A. Filtrate A is centrifuged at 4000 rpm for 15 min to obtain supernatant B and precipitate B , mix filter residue A and precipitate B, dry and pulverize with hot air to obtain a functional feed additive.

[0029] Add absolute ethanol to the supernatant B so that the volume fraction of ethanol in the mixture is 40%. After fully stirring, precipitate at 10 °C for 6 h. The mixture is centrifuged at 4000 rpm for 15 min to obtain supernatant C and precipitate C. Precipitate C was washed with 70% ethanol aqueous solution until the washing liquid was colorless, washing liquid D and precipitate D were collected, and precipitate D was vacuum-dried or freeze-dried to obtain the olive polysaccharide-protein complex.

[0030] Combine the supernatant C and the washing solution D, concentrate and recover ethanol u...

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Abstract

The invention discloses a method for extracting polyphenols from olive processing waste liquid, and belongs to the technical field of biological separation. The invention uses olive processing waste liquid as raw material, adopts ethanol precipitation technology and macroporous adsorption resin separation technology, obtains high-quality olive polyphenols, and at the same time obtains functional feed additives and olive protein-polysaccharide complexes, and realizes olive processing waste liquid The comprehensive development and utilization of olive polyphenols, and provide technical support for the production of olive polyphenols, has good economic and social effects.

Description

technical field [0001] The invention belongs to the technical field of biological separation, and relates to a method for extracting polyphenols from olive processing waste liquid, in particular to a method for separating and extracting olive polyphenols from olive processing waste liquid. technical background [0002] Plant polyphenols are a class of polyphenolic substances that widely exist in plants, mainly in bark, root, wood, leaf, and fruit of plants. They are also a class of natural products with unique physiological and pharmacological activities. A large number of studies have shown that plant polyphenols have good effects in many aspects such as anti-mutagenesis, anti-tumor, anti-virus, anti-microbial, anti-aging, anti-oxidation, etc. have a broad vision of application. In recent years, the polyphenol products that have been studied more are mainly tea polyphenols and grape seed polyphenols. Olive polyphenols are bioactive components extracted from olive leaves o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/32A61P37/04A61P39/06A61P31/12A61P31/04A61P29/00A61P1/16A61P39/02A61P3/10A61P19/10A61P31/20
Inventor 孔维宝张继白万明牛世全张文许晓卉
Owner NORTHWEST NORMAL UNIVERSITY
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