68results about How to "Good potential for industrial application" patented technology

The invention relates to a method for preparing a covalent organic framework material by high-pressure homogenization. According to the synthesis method, at normal temperature and normal pressure, a common organic ligand is used as a raw material, water or an organic reagent is used as a solvent and a template agent, organic acid and inorganic acid are used as catalysts, and the corresponding COFs are obtained by homogenizing for a certain time through a high-pressure homogenizer. The method comprises the following steps of: adding 2, 4, 6-trihydroxy-1, 3, 5-benzenetricarboxaldehyde and p-phenylenediamine into a beaker, adding a proper amount of water and acetic acid, uniformly mixing, adding the mixture into a high-pressure homogenizer, and homogenizing for a certain time; and carrying out suction filtration on the product powder after the homogenizing reaction, recycling the filtrate, washing the product powder, and carrying out vacuum drying to obtain the covalent organic framework material HPH-Tp-Pa-1. Compared with other COFs preparation methods, the method provided by the invention has the advantages of continuous reaction, simple operation, low cost, high yield, environmental protection, energy saving, short reaction time and the like, has the potential of large-scale production, and can greatly promote the industrial production process of the COFs material.

The invention provides a genetic engineering strain for producing 1-hydroxyphenazine as well as a preparation method and applications of the genetic engineering strain. The genetic engineering strain is obtained through the following method: the phzH gene in Pseudomonas chlororaphis HT66 CCTCC NO:M2013467 and the genome of the derivative of the phzH gene is replaced by the exogenous phzS gene. The preparation method comprises the following steps: construction of recombinant plasmids, biparental hybridization, and screening of gene substitution mutant strains. For the first time, the exogenous phzS gene replaces the phzH gene in the strain HT66, the obtained genetic engineering strain can produce high-concentration phenazine-1-carboxylic acid through fermentation by taking natural agricultural and sideline products as raw materials, the high-concentration phenazine-1-carboxylic acid is taken as a substrate and is converted, and thus the high-level 1-hydroxyphenazine is obtained. The genetic engineering strain is safe and reliable, is friendly to the environment, and can be used for preparing 1-hydroxyphenazine.

The invention discloses a rapid regeneration method of a positive electrode material of a waste ternary lithium ion battery. The rapid regeneration method comprises the following steps: manually disassembling the waste ternary lithium ion battery, cleaning with dimethyl carbonate, soaking with NMP, centrifuging, oxidizing, spray-drying, grinding and calcining to obtain waste ternary positive electrode material powder; a combined process of pre-oxidation treatment, spray drying and high-temperature short-time annealing is provided to regenerate the waste ternary positive electrode material; a rock salt phase on the surface of the waste ternary lithium ion battery positive electrode material is oxidized, alkali is added to generate a layered intermediate phase, lithium mixing and grinding are performed, and short-time high-temperature annealing treatment is performed, so that the intermediate phase can form a layered oxide phase to repair a failed surface; meanwhile, lithium lost in thecirculation process can be supplemented, so that the positive electrode material is regenerated; and the method provided by the invention can realize rapid repair and regeneration of the waste ternarypositive electrode material, has the characteristics of simple operation, high efficiency and economical efficiency and no pollution, and provides a new idea for recovery and regeneration of the ternary positive electrode material of the waste lithium ion battery.

The present invention relates to a method for efficiently separating and enzymolyzing lignocellulose with an ionic liquid-high boiling alcohol composite system. The method comprises the following basic steps: pulverizing lignocellulose to 20 mesh or smaller; after non-structural components are removed by one or more combinations of hot water, organic solvent and a neutral detergent, pre-treatmentis conducted with an ionic liquid-high boiling alcohol composite system; and after the treatment is completed, solid-liquid separation is performed to obtain cellulose-rich solids and a filtrate, thesolids are enzymatically hydrolyzed to obtain a sugar solution, and water is added to the filtrate to regenerate the composite system. Advantages are as follows: a series of advantages of low cost ofproton-type ionic liquid, high pretreatment separation efficiency, high boiling alcohol as a good solvent for lignin, etc. are fully combined, production costs are further reduced, high-efficient removing of the lignin is conducted, the efficient pretreatment and enzymolysis of the lignocellulose are further realized, and the method is strong in practicability and has good industrial application potential.

The invention discloses bacillus subtilis for improving the yield of 2-fucosyllactose and a construction method and application of the bacillus subtilis. Bacillus subtilis 168 serves as an original strain, a constitutive promoter Psrfa is adopted to express an exogenous gene bifunctional enzyme L-fucokinase fkp on a genome, a constitutive promoter P43 is adopted to express exogenous genes alpha-1,2-fucosyltransferase futC, lactose transporter lac12 and endogenous gene monosaccharide protease glcp on the gene respectively, and ribosome binding site RBS sequences of fkp, futC, lac12 and glcp are respectively optimized to obtain the bacillus subtilis for improving the yield of the 2'-fucosyllactose. By optimizing the RBS sequence, 2'-FL metabolic pathway key gene is systematically optimized, the initial translation rate of the gene is improved, the final 2'-FL fermentation yield reaches 1.0 g/L, the operation is simple and convenient, and the improvement effect is remarkable.

The invention discloses a chitosanase Csn-PT. The amino acid sequence of the chitosanase Csn-PT is shown as SEQ ID NO. 1. The invention further discloses a gene for coding the chitosanase Csn-PT, andthe nucleotide sequence of the gene is shown as SEQ ID NO. 2. The invention also discloses application of the chitosanase Csn-PT in degrading chitosan and application of the chitosanase Csn-PT in preparing an enzyme preparation for degrading chitosan. The chitosanase Csn-PT provided by the invention is used for degrading chitosan, has the optimum pH of 8, the optimum reaction temperature of 50 DEGC and the specific enzyme activity is 10163.826 U/mg, the chitosanase Csn-PT has high catalytic activity, and has obvious advantages in the aspects of catalytic activity and efficiency compared withreported chitosanase.

The invention discloses a preparation process of a broadband cerium oxycarbonate hydrate microwave absorbent, and belongs to the technical field of microwave absorption. The butterfly-shaped cerium oxycarbonate hydrate is rapidly synthesized by taking cerium nitrate hexahydrate and urea as raw materials and adopting a one-step microwave hydrothermal reaction. The cerium oxycarbonate hydrate microwave absorbent rapidly prepared through a one-step microwave hydrothermal method is novel in morphology and short in reaction time, the morphology and size of the product can be adjusted and controlled by changing the reaction temperature, the ratio of the amount of substance of an alkali source to the amount of substance of a cerium source, time and concentration, and the microwave absorbent has excellent microwave absorption characteristic. The controllable preparation process method disclosed by the invention is simple to operate, short in time and novel and uniform in product morphology, overcomes the characteristics of harsh reaction conditions, complex reaction process, no environmental protection, single reaction product morphology and the like in the previous preparation process, has good industrial application potential, and is expected to be popularized and applied in the market.

The invention discloses a preparation method of double-pyridine type ironic liquid. The preparation method comprises the following steps of: arranging at least one layer of cation exchange membrane inan electrolytic bath; adopting PbO2 as an anode and graphite as a cathode; taking a 2,2-bi-(4-alkyl pyridine)methyl-1,3-propylene glycol water solution of which the concentration is 2-4 mol/L as anolyte; taking an acetonitrile and water mixed solution of KBr of which the content is 8-13 wt% as catholyte; switching on the power; carrying out constant-current electrolysis for 1-5 hours under the conditions that the electrolysis temperature is 40-65 DEG C and the current density is 0.5-2 A/dm<2>; and taking out the catholyte after the electrolysis is ended so as to obtain the double-pyridine type ironic liquid. The preparation method disclosed by the invention is simple in preparation and purification processes and is also relatively high in yield; and meanwhile the prepared double-pyridinetype ironic liquid realizes good application prospects in the aspects such as catalysis and electrochemistry.

The invention discloses a method for preparing high-quality crop straw dissolved pulp through hydrothermal pretreatment. The method comprises the following basic steps: screening crushed straws to obtain a long fiber raw material; soaking the screened long fiber raw material in hot water to remove non-structural components; carrying out hydrothermal pretreatment on the water-insoluble straws, thenextracting by using an alkaline method; extracting lignin from the straws subjected to alkali treatment by using high-boiling alcohol, and bleaching the straws by using a sodium chlorite acidic solution; fully washing the bleached straws, and pulping the straws by a pulping board to obtain a finished product of straw dissolved pulp. The method has the advantages that the process is simple and easy to operate, the high-quality crop straw dissolved pulp is produced at a low cost, meanwhile, three components in the straws are effectively separated and utilized, and the production process is highin practicability and has good industrial application potential.

The invention discloses a method for efficiently converting furfural to gamma-valerolactone by using POMOF catalyst synthesis and a one-pot method. In an isopropanol solvent, the one-pot method is used for converting the furfural into the gamma-valerolactone. The reaction process comprises the following steps: putting furfural, isopropanol and POMOF into a high-pressure reaction kettle, uniformlycarrying out mixing, carrying out sealing, then carrying out stirring for 1-48 hours at a temperature of 120-180 DEG C, and carrying out cooling to obtain a gamma-valerolactone solution. The catalystused in the method is cheap, is easily available, and has stable catalytic performance and good reusability. In addition, a hydrogen environment is not needed in the whole reaction, inorganic acid isnot used, and high operation safety and very good industrial application potential are achieved.

The invention discloses a magnetic hollow micro-nano material, a preparation method and application thereof, and belongs to the technical field of nano materials. According to the magnetic hollow micro-nano material and the preparation method and application thereof, the magnetic hollow micro nano material prepared by a one-step liquid-phase reduction-oxidation method has novel structure and formation mechanism, the contents of Cu and Ni and the composition, feature, and structure of the magnetic hollow micro-nano material can be regulated by changing the reaction temperature, time, stirring speed, gas flow size, and reactant concentration, wherein the diameter of a micro-nano tube is 0.1 to 2 [mu]m, the length is 2 to 12 [mu]m, and the wall thickness is 10 to 140 nm; the diameter of a micro-nano sphere is 0.2 to 5 [mu]m, and the wall thickness is 10 to 180 nm. Because the magnetic hollow micro-nano material is good in dispersibility, good in homogeneity and has good microwave absorption characteristics, the magnetic hollow micro-nano material is widely used in the fields such as microwave absorbing and shield, electrocatalysis, lithium ion batteries and surface enhanced Raman spectroscopy. In addition, the disclosed preparation method is easy to operate, the environment-friendly effect is realized, industrial application potential is good, and the magnetic hollow micro-nano material and the preparation method are suitable for market promotion and application.

The invention discloses equipment for removing carbon dioxide from waste gas, which comprises an algae culture water tank and a purification reaction container, wherein the bottom of the purification reaction container is provided with an air inlet pipe, a water inlet pipe and a sludge discharge pipe; the top of the purification reaction container is provided with an air outlet pipe and a water outlet pipe; a water distributor, a hollow fibrous membrane assembly, a baffle plate and a light source are sequentially arranged in the purification reaction container from inside to outside; the air inlet pipe is communicated with the hollow fibrous membrane assembly, and the air inlet pipe is provided with an air inlet pump, a gas flow meter, an air inlet pressure gauge and an air inlet valve; the air outlet pipe is communicated with the hollow fibrous membrane assembly, and the air outlet pipe is provided with an air outlet pressure gauge and an air outlet valve; the water distributor is communicated with the algae culture water tank through the water inlet pipe, and the water inlet pipe is provided with a water inlet pump, a liquid flow meter and a water inlet valve; the algae culture water tank is communicated with the purification reaction container through the water outlet pipe; the sludge discharge pipe is provided with a sludge discharge pump and a sludge discharge valve; and the light source is connected with a power supply. The equipment has reasonable design, strong applicability, long service life, high carbon dioxide removal efficiency and good industrialized application potential.

The invention discloses a cyanine near-infrared fluorescent probe as well as a preparation method and application thereof, the cyanine near-infrared fluorescent probe disclosed by the invention takes hydrolysis of ester bonds bonded in cyanine dye by esterase as a basic action mechanism, and takes H-type aggregate luminescence of cyanine-amino alcohol as a fluorescence signal reporter group. When esterase is added into a system, ester bonds in a Cy-NE structure can be cut off by the esterase, and Smiles rearrangement reaction is caused by O-ions, so that the color and fluorescence of the solution are changed. The recognition effect of the cyanine near-infrared fluorescent probe on esterase is researched through a colorimetric method, an ultraviolet-visible absorption spectrometry and a fluorescence spectrometry, and the result shows that the near-infrared fluorescent probe can efficiently and selectively recognize esterase in a PBS solution system and has very high response sensitivity on esterase.

The invention discloses a synthesis method of 5-methyl-2-pyrrolidone or derivatives thereof, the synthesis method comprises the following steps: fully mixing levulinic acid (ester) with urea or amideand formic acid, and adding the mixture and a catalyst into a high-pressure reaction kettle for reaction to obtain the target product 5-methyl-2-pyrrolidone or derivatives thereof. After the reactionis completed, the catalyst is filtered and recovered, cleaned and dried before reuse. The method can realize efficient conversion of the levulinic acid (ester) in a solvent-free system, the low-cost and environment-friendly urea or amide is taken as a N source, and the biomass formic acid is taken as a H source, so that the synthesis method has good economy.