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Method for extracting saponins of traditional Chinese medicine

A technology of saponins and traditional Chinese medicine, which is applied to medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulas, etc. It can solve the problems of inconvenient industrial production, large loss of active ingredients, and high polarity. Achieve the effects of easy industrialized large-scale production, high content of saponins, and high extraction and separation efficiency

Inactive Publication Date: 2011-09-21
TIANJIN TASLY ZHIJIAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the extraction and separation of saponins, the application of chromatography methods such as reversed-phase silica gel chromatography, gel chromatography, droplet countercurrent distribution chromatography, and macroporous resins has greatly improved the efficiency of the extraction and separation of saponins. , but due to the large molecular weight and high polarity of saponins, the existing saponin process is relatively cumbersome, the loss of active ingredients is large, and it is not convenient for industrial production. Therefore, it is important to study more effective and high-purity extraction and separation methods for saponins. significance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take Bupleuri herb, crush it, add water to boil and extract three times and control the pH, the first time is 6 times the amount of water and 0.7% of the amount of Na2CO3, the second time is 4 times the amount of water and 0.3% of the amount of Na2CO3 , the third time is 4 times the amount of water of the medicinal material; side countercurrent circulation extraction, after boiling, keep for 1.5, 1, 1 hour respectively; the medicinal liquid is combined and cooled to room temperature, the medicinal liquid is concentrated to 2 times the volume of the medicinal material, and ethanol is added to make the alcoholic The amount reaches 70%, let it stand for 24 hours, filter, the filtrate decompresses and recovers ethanol until it has no alcohol smell, dissolves it in water, and filters; the macroporous weakly basic anion D382 exchange column (Nankai University Chemical Plant) that has been treated on the filtrate ), rinsed with 2% NaOH solution of 2 times the volume of the resi...

Embodiment 2

[0017] Take the residue after extracting volatile oil from Bupleuri, add 50% ethanol to reflux extraction three times and control the pH, the first time is 5 times the amount of 50% ethanol and 1% Na2CO3 of the amount of the medicinal material, and the second time is 4 times the amount of the medicinal material 50% ethanol and 0.5% medicinal Na2CO3, the third time is 4 times the amount of medicinal materials and 50% ethanol; side countercurrent circulation reflux extraction, after the reflux starts, keep it for 1.5, 1, 1 hour respectively; filter the medicinal liquid and combine the filtrate , after the filtrate was concentrated under reduced pressure at 70°C to no alcohol smell, the concentrated solution was diluted with water to a ratio of medicinal material to liquid of 1:1.5, filtered, and the filtrate was treated with a macroporous strong basic anion D201 exchange column (Nankai University Chemical Industry Co., Ltd. factory), washed with 2% NaOH solution 2 times the volum...

Embodiment 3

[0019] Get Acanthopanax medicinal materials (roots, rhizomes, stems), pulverize, add 50% ethanol to reflux and extract three times and control the pH, the first time is 5 times the Na2CO3 of 50% ethanol amount and 1% medicinal material amount, and the second time 4 times the amount of 50% ethanol and 0.5% Na2CO3 of the amount of medicinal materials, the third time is 4 times the amount of 50% ethanol of the amount of medicinal materials; side countercurrent circulation reflux extraction, after the reflux starts, keep it for 1.5, 1, and 1 hour respectively; Filter the medicinal liquid and combine the filtrates, concentrate the filtrate under reduced pressure at 70°C until there is no alcohol smell, dilute the concentrated liquid with water until the ratio of medicinal materials to liquid is 1:1.5, filter, and the treated macroporous strong basic anion D201 on the filtrate Exchange column (Nankai University Chemical Plant), rinse with 2% NaOH solution of 2 times the volume of the...

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Abstract

The invention discloses a preparation method of saponins from medicinal herbs which comprises, adding medicinal herbs into solvent, regulating to alkality, extracting, loading on macroporous basic anion-exchange column, eluting with alkali solution and pure water, discarding the flushing liquor, eluting with aqueous ethanol to give eluant, and concentrating.The inventive extraction method has theadvantages of high extraction efficiency, high saponins content, good biological activity presevation, and easy industrialization.

Description

technical field [0001] The invention belongs to the extraction method of traditional Chinese medicine extract, in particular, relates to the extraction method of traditional Chinese medicine saponin components. Background technique [0002] Saponins are a kind of complex glycoside compounds, which are composed of saponins and sugar, uronic acid or other organic acids. Common sugars in saponins include glucose, galactose, rhamnose, arabinose, xylose and other pentose sugars; common uronic acids include glucuronic acid, galacturonic acid, etc. These sugars or uronic acids are first combined into sugar chains and then linked to saponins. According to the chemical structure of aglycones, they can be divided into two categories: triterpene saponins and steroidal saponins. The saponins of triterpene saponins are triterpenoid compounds, which are composed of 30 carbon atoms except for a few. The basic skeleton can be divided into two categories: pentacyclic triterpenes and tetrac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/00C07H1/08
Inventor 杨悦武叶正良郑永锋李旭
Owner TIANJIN TASLY ZHIJIAO PHARMA
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