Method for preparing total saponins of astragalus

A technology of astragalus total saponins and astragalus, which is applied in the field of preparation of traditional Chinese medicine extracts, can solve the problems of complex operation steps, large loss of astragalus saponins, and low purity, and achieve high physiological activity, high extraction and separation efficiency, and high content.

Inactive Publication Date: 2011-09-21
TIANJIN TASLY ZHIJIAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the content of astragaloside is low, and the root contains a lot of sugar substances, so it is difficult to extract and separate
At present, the preparation methods of astragaloside mainly include water extraction and alcohol precipitation method, and the alcohol solution is used to separate saponins. With this method, astragaloside is greatly lost, the yield is low, and the production cost is high; it can also be extracted with alcohol organic solvents. , after concentration, pass through a macroporous adsorption resin column, and elute gradiently with water and alcohol solutions of different concentrations. Using this method, the operation steps are complicated, the impurity content is high, and the purity is low. Therefore, it is more effective and high-purity to study Astragalus The extraction and separation method of saponin is of great significance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take the medicinal material of Astragalus membranaceus, crush it, add water to boil and extract three times and control the pH, the first time is 6 times the amount of water and 0.7% of the amount of Na2CO3, the second time is 4 times the amount of water and 0.3% of the amount of Na2CO3, The third time is 4 times the water volume of the medicinal material; side countercurrent circulation extraction, and keep it for 1.5, 1, and 1 hour respectively after boiling; the medicinal liquid is combined and cooled to room temperature, filtered, and the macroporous weakly basic anion D392 that has been treated on the filtrate Exchange column (Nankai University Chemical Plant), rinse with 2% NaOH solution of 2 times the volume of the resin bed, discard the rinse solution; rinse with pure water 3 times, discard the rinse solution with water; use 70% ethanol with 7 times the volume of the resin bed Elution, collect the eluate, concentrate to a thick paste (relative density at 50°C is ...

Embodiment 2

[0017] Take the medicinal material of Radix Astragali, crush it, add water and ultrasonically extract it four times and control the pH, the first time is 5 times the amount of water and 0.9% of the amount of NaHCO3, the second time is 4 times the amount of water and 0.5% of the amount of NaHCO3 , the third and fourth times are 3 times the amount of water; the medicinal liquid is combined and cooled to room temperature, filtered, and the treated macroporous type weakly basic anion D380 exchange column (Nankai University Chemical Plant) on the medicinal liquid filtrate, Rinse with 2.5% NaOH solution 1.5 times the volume of the resin bed, discard the rinse solution; then rinse with pure water 4 times, discard the water rinse solution; elute with 65% ethanol 8 times the volume of the resin bed, collect the eluate, and concentrate To a thick paste (relative density at 50° C. is 1.28 to 1.33), dry with a dynamic belt drying device to obtain total saponins of Astragalus (87.5% total s...

Embodiment 3

[0019] Take the medicinal material of Astragalus membranaceus, crush it, add 50% ethanol to reflux and extract three times and control the pH, the first time is 5 times the amount of the medicinal material, 50% ethanol and 1% Na2CO3, and the second time is 4 times the amount of the medicinal material 50% ethanol amount and 0.5% Na2CO3 of the amount of medicinal material, the third time is 4 times the amount of 50% ethanol of the amount of medicinal material; side countercurrent circulation reflux extraction, after the reflux starts, keep respectively for 1.5, 1, and 1 hour; filter the medicinal liquid and combine the filtrate, the filtrate 70 After concentrating under reduced pressure at ℃ until there is no alcohol smell, the concentrated solution is diluted with water until the ratio of medicinal materials to liquid is 1:1.5, filtered, and the filtrate is treated with a macroporous strong basic anion D201 exchange column (Nankai University Chemical Factory), Rinse with 2% NaOH...

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Abstract

The invention discloses a process for preparing astragalus root saponins comprising the following steps in sequence, charging dissolvent into astragalus root, adjusting the solution to alkalinity, carrying out extraction, obtaining extract, passing the extract through macroscopic alkaline anion exchange resin, flushing with alkaline water and pure water, discarding the flushing fluid, eluting with water-containing ethanol, and concentrating the eluent.

Description

technical field [0001] The invention belongs to a preparation method of a traditional Chinese medicine extract, in particular to a preparation method of total astragalus saponins. Background technique [0002] Astragalus membranaceus (Fisch) Bge.Var.mongholicus (Bge.) Hsiao or Astragalus membranaceus (Fisch) Bge. is the dry root of the leguminous plant Astragalus membranaceus (Fisch) Bge. It is a commonly used tonic, sweet in taste, slightly warm in nature, traditional Medicine believes that it has the effects of invigorating qi and strengthening the surface, diuretic and detoxification, astringing sores and promoting granulation, and has a long history of medicinal use. Modern chemical research has proved that Astragalus contains saponins, polysaccharides, flavonoids, amino acids, phenolic compounds and trace elements. Astragaloside has a strong heart function and can be used to treat heart failure, and has a significant effect on the recovery of heart function after acute...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/481A61P9/04A61K125/00
Inventor 杨悦武叶正良郑永锋李旭
Owner TIANJIN TASLY ZHIJIAO PHARMA
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