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Method for preparing total saponins of radix ophiopogonis

A technology for total saponins of Ophiopogon japonicus and Ophiopogon japonicus, which is applied in the field of preparation of traditional Chinese medicine extracts, can solve the problems of large loss of effective components of Ophiopogon japonicus, cumbersome processes, low purity of extracts, etc., and achieves easy industrialized large-scale production, high physiological activity, High extraction and separation efficiency

Inactive Publication Date: 2007-12-12
TIANJIN TASLY ZHIJIAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used methods for extracting Ophiopogon japonicus saponins from Ophiopogon japonicus include water extraction, alcohol extraction, and macroporous adsorption resin method, etc., but the purity of the obtained extract is not high, the process is cumbersome, and the active ingredients of Ophiopogon japonicus are lost. Therefore, it is of great significance to study more effective and high-purity extraction and separation methods of Ophiopogon japonicus saponins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take Ophiopogon japonicus medicinal material, pulverize, add water and boil to extract three times and control the pH, the first time is 6 times the amount of medicinal material and 0.7% Na2CO3, the second time is 4 times the medicinal material amount of water and 0.3% Na2CO3 , the third time is 4 times the water volume of the medicinal material; side countercurrent circulation extraction, after boiling, keep for 1.5, 1, and 1 hour respectively; the medicinal liquid is combined and cooled to room temperature, filtered, and the macroporous weakly basic anion that has been treated on the filtrate D392 exchange column (chemical factory of Nankai University), rinse with 2% NaOH solution of 2 times of resin bed volume, and discard the rinse solution; then rinse with 3 times of pure water, discard the water rinse solution; use 70% of the resin bed volume of 7 times Elute with ethanol, collect the eluate, concentrate to a thick paste (relative density at 50°C is 1.28-1.33), and...

Embodiment 2

[0017] Take Ophiopogon japonicus medicinal material, pulverize, add water and ultrasonically extract four times and control the pH, the first time is 5 times the amount of water and 0.9% of the amount of NaHCO3, the second time is 4 times the amount of water and 0.5% of the amount of the drug NaHCO3, the third and fourth times are 3 times the water volume of the medicinal material; the medicinal liquid is combined and cooled to room temperature, filtered, and the macroporous weakly basic anion D380 exchange column (Nankai University Chemical Factory) that has been treated on the medicinal liquid filtrate , wash with 2.5% NaOH solution of 1.5 times the volume of the resin bed, and discard the washing solution; rinse with 4 times of pure water, discard the water washing solution; elute with 65% ethanol of 8 times the volume of the resin bed, collect the eluent, Concentrate to a thick paste (relative density is 1.28-1.33 at 50° C.), and dry with dynamic belt drying equipment to ob...

Embodiment 3

[0019] Take Ophiopogon japonicus medicinal material, pulverize, add 50% ethanol to reflux and extract three times and control the pH, the first time is 5 times the amount of medicinal material 50% ethanol and 1% Na2CO3, the second time is 4 times the amount of medicinal material 50% The amount of ethanol and Na2CO3 of 0.5% medicinal material amount, the third time is 4 times of the medicinal material amount 50% ethanol amount; side countercurrent circulation reflux extraction, and keep 1.5, 1, 1 hour respectively after the reflux begins; medicinal liquid filtration and combined filtrate, filtrate Concentrate under reduced pressure at 70°C until there is no alcohol smell, dilute the concentrated solution with water until the ratio of medicinal materials to liquid is 1:1.5, filter, and put the treated macroporous strong basic anion D201 exchange column on the filtrate (Nankai University Chemical Factory) , washed with 2% NaOH solution of 2 times the volume of the resin bed, and d...

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PUM

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Abstract

The invention discloses a preparation method of total saponins from Ophiopogon japonicus, which comprises in sequence: adding Ophiopogon japonicus into solvent, regulating to alkality, extracting, loading on macroporous basic anion-exchange column, eluting with alkali solution and pure water, discarding the flushing liquor, eluting with aqueous ethanol to give eluant, and concentrating. The inventive preparation method of total saponins of Ophiopogon japonicus has high extraction separation efficiency, high total saponins content, high biological activity, and easy industrialization production.

Description

technical field [0001] The invention belongs to a preparation method of a traditional Chinese medicine extract, in particular to a preparation method of total saponins of Ophiopogon japonicus. Background technique [0002] Ophiopogon japonicus (Thunb.) Ker-Gawl. is the dry tuber root of Ophipogon japonicus (Thunb.) Ker-Gawl., a plant of the genus Ophiopogon japonicus (Thunb.) Ker-Gawl. Upset thirst embolism. Ophiopogon japonicus and its preparations have certain anti-myocardial hypoxia and anti-arrhythmia effects, and have achieved certain curative effects in clinical use for coronary heart disease and angina pectoris. The main active ingredient of Ophiopogon japonicus is Ophiopogon japonicus saponin, which is composed of a variety of steroidal saponins. The aglycones are Ruscogehin and Diosgenin. 23 saponins have been isolated. Modern research has found that Ophiopogon japonicus has the effects of strengthening myocardial contractility and enhancing coronary artery flow. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8968A61P9/10A61P9/06A61K125/00
Inventor 杨悦武叶正良郑永锋李旭
Owner TIANJIN TASLY ZHIJIAO PHARMA
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