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Preparation method and application of Jasminum lanceolarium medium-ring iridoids

A technology of iridoids and terpenoids, which is applied in the field of medicine, can solve problems such as poor stability, and achieve the effects of high extraction and separation efficiency, strong operability, and simple and reliable preparation methods

Active Publication Date: 2013-02-27
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The stability of iridoids is very poor, basically existing in the form of glycosides

Method used

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  • Preparation method and application of Jasminum lanceolarium medium-ring iridoids
  • Preparation method and application of Jasminum lanceolarium medium-ring iridoids
  • Preparation method and application of Jasminum lanceolarium medium-ring iridoids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Preparation of iridoids from fragrant canes

[0044] (1) Use the solvent extraction method after the cane of Fenxiang fragrance is pulverized, adopt 70% ethanol to reflux extract three times, recover the extract under reduced pressure until the alcohol concentration is lower than 5%, and obtain the total extract;

[0045] (2) The total extract was treated with macroporous adsorption resin (HPD-100), and then gradient eluted with water and 40%, 60%, and 90% ethanol in sequence. As a developing agent, sulfuric acid-ethanol solution is used as a chromogenic agent to screen and determine the effective parts enriched in iridoid components in 60% and 90% ethanol elution parts to obtain a crude extract;

[0046] (3) The crude extracts eluted with 60% and 90% ethanol were processed by normal phase silica gel column chromatography, and gradient eluted with different ratios of chloroform-methanol as the eluent, and each eluted fraction was processed by thin-layer chro...

Embodiment 2

[0051] Example 2 Preparation of iridoids from fragrant canes

[0052] (1) Use the solvent extraction method after the cane of the fragrance is crushed, and use 50% ethanol to reflux to extract three times. The extract is recovered under reduced pressure until the alcohol concentration is lower than 5%. The extract is extracted with ethyl acetate and n-butanol respectively, and the solvent is recovered , collect ethyl acetate extract, n-butanol extract;

[0053] (2) Ethyl acetate extract and n-butanol extract were respectively processed by macroporous adsorption resin (D101) column chromatography, followed by gradient elution with water, 40%, 60%, and 90% ethanol, and each elution site was separated by thin layer Chromatographic technique (detection at 210nm), screening and determining the elution sites of 60% and 90% ethanol of the macroporous adsorption resin of the ethyl acetate extract and 40% and 60% ethanol elution of the macroporous adsorption resin of the n-butanol ext...

Embodiment 3

[0058] Example 3 Klebsiella pneumoniae bacteriostatic test of iridoids from fragrant canes.

[0059] The experiment was divided into DMSO-bacteria solution group, bacteria solution group, antibiotic-bacteria solution group, and sample-bacteria solution group; except for the sample-bacteria solution group, each group had 7 attached holes for parallel experiments, and DMSO, antibiotics, The amount of samples is 2.5ul (only 100ul of bacterial solution is added to each well of the sterilization solution group), and 97.5ul of bacterial solution is added to each of the remaining attached wells. The 96-well plate was placed in a shaker at 37°C and cultured statically for 16 hours, and the OD value of the sample (at 620nm) was measured with a microplate reader.

[0060] The antibacterial activity was calculated according to the following formula:

[0061] Bacteriostatic activity=1 - (OD 样品-菌液组 -OD DMSO-细菌组 ) / OD DMSO-细菌组

[0062] The test results are shown in Table 3.

[0063] ...

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Abstract

The invention relates to a preparation method of Jasminum lanceolarium medium-ring iridoids and application of the Jasminum lanceolarium medium-ring iridoids in inflammation resistance and tumor resistance, belonging to the technical field of medicine. The preparation method comprises the following steps: pulverizing dry Jasminum lanceolarium stem, carrying out flash extraction to obtain a total extracting solution, and carrying out macroporous resin column chromatography on the total extracting solution to obtain the crude extract containing rich iridoids; and separating by normal-phase silica gel column chromatography, screening the effective fraction containing rich iridoids, separating the effective fraction by semipreparative or preparative HPLC (high performance liquid chromatography), and eluting by using methanol-water, acetonitrile-water or methanol-acetonitrile-water as the mobile phase to obtain the iridoids. The preparation method provided by the invention is convenient and reliable, has the advantages of high extraction separation efficiency and strong operability, and can easily implement industrialized mass production; and the prepared iridoids have anti-inflammatory and antitumor activities.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to a preparation method of iridoid compounds in fenugreek vine and anti-inflammatory and anti-tumor applications. Background technique [0002] Fragrant Vine ( Jasminum lanceolarium Roxb.), also known as Chuanxiangjasmine, Guangqingxiangteng, and Beiqingxiangteng, is a plant of the genus Frangipani in the family Oleaceae. The medicinal parts of Qingxiangteng are roots and stems, which are called broken bones. According to "Compendium of Xinhua Materia Medica", it is recorded that Cragoptera has the functions of expelling wind and dampness, cooling blood and detoxifying, and it is mainly used for rheumatic arthralgia, bruises, headache, traumatic bleeding, nameless sores, and snakebites. In our country, the rhizome of fennel is used to treat fever and rheumatic pain, and its leaves can be used to reduce inflammation and treat eye pain. [0003] At present, there are few reports ab...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08A61K31/7048A61P31/04A61P29/00A61P35/00
Inventor 孟大利张辘辘陈超徐丽焕
Owner SHENYANG PHARMA UNIVERSITY
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