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Reagent kit for predicting serotonin re-uptake inhibitor medicine effect

A technology for serotonin reuptake and drug action, applied in the field of medicine and biology, can solve problems such as inability to tailor, patient economic loss, fluoxetine blood concentration difference, etc., and achieves a simple and reliable preparation method, reduces medical costs, and reduces toxic and side effects. Effect

Active Publication Date: 2007-09-05
深圳泰乐德医疗有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Individual differences in CYP 2D6 enzyme activity apparently lead to differences in plasma concentrations of fluoxetine and other antidepressants metabolized by CYP2D6
This may affect the efficacy and adverse reactions of the drug
However, so far no studies have found a positive correlation between fluoxetine blood levels and its antidepressant clinical efficacy
[0013] Due to individual differences in drug response, doctors cannot choose drugs and drug compatibility according to the individual's needs when choosing drugs, and cannot improve the efficacy of drugs and reduce the occurrence of toxic and side effects, which may delay the timing of treatment and cause economic losses to patients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1: A kit for determining the genotype of the HTR1A C1019G (rs6295) polymorphic locus to predict the effect of serotonin reuptake inhibitor drugs (PCR-RFLP method)

[0058] (1) Components of the kit:

[0059] (1) Red blood cell lysate: NH 4 Cl, KHCO 3 , EDTA;

[0060] (2) White blood cell lysate: proteinase K, RNase A, NaCl, Tris, EDTA, SDS;

[0061] (3) Protein precipitation solution: 7.5M ammonium acetate (pH 7.4);

[0062] (4) Nucleic acid storage solution: 1M tris-hydrochloric acid (Tris-HCl, pH8.0);

[0063] (5) 2×PCR reaction mixture: 1.0ml [100mM Tris-HCl, 100mM KCl, pH8.3 (20°C)]; 5.0uM MgCl2; each 0.4mM dATP, dCTP, dGTP, dTTP, sterile double distilled water Preparation, pH 7.0; primers for genotype detection of polymorphic sites of HTR1A gene; internal standard; wherein the primer sequence was designed according to the gene sequence of HTR1A C1019G (rs6295), including PCR forward primer and PCR reverse primer, provided by Shanghai Sangong Co., Ltd. ...

Embodiment 2

[0106] Example 2: A kit for determining the genotype of the HTR1A C1019G (rs6295) polymorphic locus to predict the effect of serotonin reuptake inhibitor drugs (Taqman method)

[0107] (1) The main components of the kit:

[0108] (1) Red blood cell lysate: NH 4 Cl, KHCO 3 , EDTA;

[0109] (2) White blood cell lysate: proteinase K, RNase A, NaCl, Tris, EDTA, SDS;

[0110] (3) Protein precipitation solution: 7.5M ammonium acetate (pH 7.4);

[0111] (4) Nucleic acid storage solution: 1M tris-hydrochloric acid (Tris-HCl, pH8.0);

[0112] (5) Taqman 2×Universal PCR Master Mix No AmpErase UNG reaction mixture: dNTPs containing dUTP, optimized buffer, 0.72uM forward primer, 0.72uM reverse primer and two fluorescent reporter groups Allele-specific probes were 0.16uM each.

[0113] The primer sequence was designed according to the HTR1A C1019G (rs6295) gene sequence, including PCR forward primer and PCR reverse primer, which were synthesized by Shanghai Sangong Company:

[0114]...

Embodiment 3

[0155] Example 3: Predicting the drug effect of depression patients taking serotonin reuptake inhibitor drugs using the test kit produced in a small test

[0156] We used the kit produced in a small laboratory to detect the polymorphic site genotype of HTR1A C1019G (rs6295) in patients with depression who took serotonin reuptake inhibitor drugs, and at the same time detected other physiological parameters, such as age, Gender, height, weight, smoking history, drinking history, occupation, education level, baseline HAMD total score, current course of disease, previous history of antidepressant drug treatment, combined medication, relapse of depression, etc. Predict the effect of serotonin reuptake inhibitor drugs by measuring individual genotype parameters and other physiological parameters:

[0157]1. Obtain the individual's HTR1A C1019G (rs6295) polymorphic locus genotype parameters and basic physiological parameters: age, sex, height, weight, smoking history, drinking histor...

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PUM

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Abstract

Reagent kit for predicting 5-hydroxytryptamine re-ingestion inhibitor medicinal effect contains erythrocyte lytic liquid, leukocyte lytic liquid, protein deposit, nucleic acid reserve liquid, PCR reactive mixed liquid with MgC12, dNTP and PCR cloning primer and indicator, DNA polymerase, positive proton, negative control, endonuclease buffer system, limited endonuclease, PCR water, 10X electrophoretic buffer liquid, bromphenol blue 0.25% and sugar solution w / v40%. It extracts gene DNA in biological sampler and has polymerase chain-reaction-limited enzyme fragment polymorphism PCR-RFLP analysis.

Description

technical field [0001] The invention relates to a kit for predicting the effect of serotonin reuptake inhibitor drugs, which belongs to the field of medical biotechnology. Background technique [0002] Depression is one of the most common mental disorders. For the treatment of depression, the most commonly used method is still drug treatment. Antidepressant effects are mainly related to the effects of these drugs on the 5-hydroxytryptamine (5-HT) and norepinephrine (NE) systems. There are many types of antidepressants, mainly in the following categories: serotonin reuptake inhibitors; norepinephrine reuptake inhibitors; serotonin / norepinephrine dual reuptake inhibitors; tricyclic antidepressants; others Cyclic antidepressants; monoamine oxidase inhibitors (classic / selective); other unclassified drugs. Serotonin reuptake inhibitors include selective serotonin reuptake inhibitors (selective serotonin reuptake inhibitors, SSRIs) and antidepressants that act on other pathways ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A61K45/00A61P25/24A61P25/22A61P25/20A61P15/10G01N33/53
Inventor 刘平王玉洪秀梅张善春王滨燕王燕徐希平
Owner 深圳泰乐德医疗有限公司
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