Preparation method and application of flaxseed polysaccharides with antiviral and immune activity

An immune activity, flaxseed technology, applied in the field of biomedicine and functional food, can solve the problem of no sulfate group and triple helix advanced structure found in flaxseed polysaccharide, so as to improve the immune function of the body, improve the extraction efficiency, and improve the extraction efficiency. The effect of shortening time
CN105037573AActive Publication Date: 2015-11-11JINAN UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JINAN UNIVERSITY
Publication Date
2015-11-11

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Abstract

The invention discloses a preparation method and an application of flaxseed polysaccharides with antiviral and immune activity. The preparation method adopting flaxseeds as a raw material comprises the following steps: crushing flaxseeds, carrying out shell-kernel separation, carrying out microwave assisted hot water extraction, carrying out Sevage technology protein removal, carrying out ethanol precipitation, freeze-drying to obtain crude flaxseed polysaccharides, and carrying out ion exchange column chromatography, carrying out Sephadex column chromatography, and carrying out ultrafiltration to prepare flaxseed sulfated polysaccharides. FHP-1 obtained in the invention has homogeneous composition, and the molecular weight is 2626kDa. Polysaccharide with sulfate and triple-helical structure are separated from the flaxseeds in the invention for the first time. Cell biology experiments prove that the polysaccharides can reduce expression of hepatitis B surface antigen and hepatitis B e antigen and inhibit replication of hepatitis B viruses, can activate immune response, and improves secretion of the tumor necrosis factor TNF-a, interleukins IL-6 and IL-12, and an inflammation factor NO by immune cells.
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Description

technical field

[0001] The invention belongs to the fields of biomedicine and functional food, and in particular relates to the preparation and application of a flaxseed polysaccharide with antiviral and immune activities. Background technique

[0002] Flax (Linumustitatissimum L.), also known as flax, belongs to the flax family and the genus flax. As a traditional oil crop, flaxseed is rich in unsaturated fatty acids, essential amino acids, vitamin E and sugar compounds and other nutrients, among which polysaccharides mainly exist in flaxseed hulls, and the content varies with the place of origin and variety rather different.

[0003] Polysaccharide, also known as polysaccharide (Polysaccharide), is a polymer compound formed by connecting more than 10 monosaccharides through glycosidic bonds, and its relative molecular mass is tens of thousands to millions. Polysaccharides are widely distributed in nature and exist in higher plants, algae, fungi and animals, such as funga...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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