Sealwort planting method
A planting method and technology of Polygonatum chinensis, which are applied in the fields of botanical equipment and methods, plant cultivation, fertilizer made of biological waste, etc., can solve the problem that the mining and mining technology of Polygonatum chinensis is not very mature, the origin of Polygonatum chinensis is difficult to find, and the secondary excavation of Polygonatum chinensis is difficult, etc. To achieve the effect of promoting industrial structure adjustment, promoting promotion and demonstration, and improving planting efficiency
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Embodiment 1
[0052] 1. Propagation of seedlings
[0053] Select and optimize the induction medium, proliferation medium and rooting medium; use the stem section with buds as the explants for the initial culture, and go through the establishment of sterile materials-bud induction-bud proliferation-rooting culture-transplant acclimatization, etc. In this process, the in vitro regeneration and breeding system of Polygonatum rhizome tissue was established.
[0054] Induction of buds: After cleaning, disinfecting and sterilizing the primary buds of Polygonatum rhizomes, they are inoculated in induction medium to induce buds for 9 days;
[0055] Proliferation and cultivation of buds: inoculate the induced buds into the proliferation medium to carry out proliferation and cultivation of buds, and cultivate for 27 days;
[0056] Rooting culture: cut off the rhizome buds of Polygonatum rhizomes cultured for proliferation, inoculate into rooting medium and carry out rooting culture for 7 days.
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Embodiment 2
[0077] In this example, sorghum was interplanted, and the others were the same as in Example 1. The yield of Polygonatum was 10% lower than that in Example 1, the total saponin content was 12.0 mg / g, and the polysaccharide content was 15.1 mg / g.
Embodiment 3
[0079] 1. Propagation of seedlings
[0080] Adopt Polygonatum seeds to propagate rapidly, the method is as follows:
[0081] A. Fermentation and rinsing treatment of Polygonatum fruit to obtain seeds: pick the ripe fruit of Polygonatum in November, put it in a sealed bag to ferment for 10 days, then take it out and rub it for rinsing.
[0082] B. Seeds are stored in low-temperature sand, and the treated seeds are mixed with wet sand, and refrigerated at 0-4°C for 120 days.
[0083] C. The refrigerated seeds are treated with ultrasonic treatment and plant growth regulator: ultrasonic treatment is mainly to reduce the mechanical barrier caused by endosperm and seed coat at the end of the hole, improve the amylase activity of Polygonatum seed, and solve the problem of limiting the formation of Polygonatum primary rhizome; plant Growth regulator treatment is mainly to break the dormancy of Polygonatum seeds to a certain extent and promote seed germination.
[0084] The germinati...
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