The invention discloses a composition capable of invigorating qi for ascending and enhancing immunity, and a preparation method thereof. The composition comprises the following ingredients: 30-40g of ginseng, 10-15g of cordyceps sinensis or cordyceps militaris, 2-3g of lycopene, 10-15g of lucid ganoderma, 10-15g of morinda officinalis, 10-15g of barbary wolfberry fruit, 50-70mcg of organic selenium, 550-650mcg of vitamin A, 5-6mcg of vitamin B, 130-140mcg of vitamin C, and 5-8g of other auxiliary materials. According to the composition and the preparation method disclosed by the invention, the extracts of the used raw materials are processed by a nanometre wall-breaking technology into micromolecules capable of being absorbed by a human body, thus greatly increasing the comprehensive utilization rate of precious food materials, providing scientific basis for industrialized and standardized production, enabling the product quality to be controllable and adequately exerting the curative effect. The composition disclosed by the invention has the functions of invigorating qi for ascending, enhancing immunity, resisting oxidation and ageing, relieving physical fatigue, assisting to improve memory, tonifying kidneys, and the like. The product is easily acceptable in taste, simple and natural in colour, beautiful and graceful, and suitable for all ages.