The invention relates to a detection method of an infantile cough and
asthma composition, and belongs to the technical field of detection. The detection method of the infantile cough and
asthma composition comprises the following steps: S1, adding the infantile cough and
asthma composition into
methanol for
reflux, cooling and filtering to obtain a to-be-detected solution; s2, determining the liquid to be detected by adopting a
high performance liquid chromatography to obtain the contents of
aesculin and
aesculetin in the infantile cough and asthma composition, according to the
high performance liquid chromatography, a
phosphoric acid aqueous solution is used as a mobile phase A,
methanol is used as a mobile phase B, and specific
elution conditions are adopted. The
aesculin and
aesculetin obtained by the detection method for the infantile cough and asthma composition are good in peak pattern and can be well separated from front and rear peaks, the detection method which is high in accuracy, good in precision, good in durability and suitable for large-scale
production quality control is provided, and detection of main components of the infantile cough and asthma composition can be achieved.