Colla corii asini low-molecule peptide as well as preparation method and application thereof

A low-molecular-weight peptide and donkey-hide gelatin technology, applied in the field of donkey-hide gelatin preparations, can solve the problems of undetectable traditional Chinese medicine odor, temporary paralysis of taste buds, masking, etc., to avoid indigestion, inhibit bitter taste, and reduce disgust.

Inactive Publication Date: 2018-07-31
青岛霍中医生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The complexity and diversity of traditional Chinese medicine components make it difficult to eliminate the odor of traditional Chinese medicine by eliminating bad odor compounds
At present, adding flavoring agents is usually used to adjust, cover up the smell of traditional Chinese medicine or temporarily paralyze the taste buds so that the smell of traditional Chinese medicine cannot be detected

Method used

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  • Colla corii asini low-molecule peptide as well as preparation method and application thereof
  • Colla corii asini low-molecule peptide as well as preparation method and application thereof
  • Colla corii asini low-molecule peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A low-molecular-weight peptide of donkey-hide gelatin, the preparation method of which is:

[0058] (1) Resin pretreatment: first soak 24h with 95% ethanol, remove the particles with poor quality floating on the liquid surface, then, soak 24h with 0.2mol / LHCl and 4% NaOH solution respectively, to remove resin Various organic substances mixed in the synthesis process were finally washed with deionized water until neutral for later use;

[0059] (2) 1000 g of donkey-hide gelatin was added with 7 times the amount of ultra-pure water to melt the gelatin to prepare a donkey-hide gelatin solution;

[0060](3) Adjust the pH of donkey-hide gelatin solution to 8.5, add 0.06% nattokinase by total weight, hydrolyze at 37°C for 3h, and treat at 100°C for 15min;

[0061] (4) Adjust the pH of the donkey-hide gelatin solution after enzymolysis to 4.0, add a total weight of 0.5% Aspergillus niger acid protease, hydrolyze at 33°C for 4 hours, and treat at 100°C for 15 minutes;

[0062...

Embodiment 2

[0066] A low-molecular-weight peptide of donkey-hide gelatin, the preparation method of which is:

[0067] (1) Resin pretreatment: first soak 24h with 95% ethanol, remove the particles with poor quality floating on the liquid surface, then, soak 24h with 0.2mol / LHCl and 4% NaOH solution respectively, to remove resin Various organic substances mixed in the synthesis process were finally washed with deionized water until neutral for later use;

[0068] (2) 1000 g of donkey-hide gelatin was added with 6 times the amount of ultra-pure water to melt the gelatin to prepare a donkey-hide gelatin solution;

[0069] (3) Adjust the pH of donkey-hide gelatin solution to 8.5, add 0.01% nattokinase by total weight, hydrolyze at 37°C for 3h, and treat at 100°C for 15min;

[0070] (4) Adjust the pH of the donkey-hide gelatin solution after enzymolysis to 4.0, add a total weight of 0.6% Aspergillus niger acid protease, hydrolyze at 33°C for 4 hours, and treat at 100°C for 15 minutes;

[007...

Embodiment 3

[0075] A low-molecular-weight peptide of donkey-hide gelatin, the preparation method of which is:

[0076] (1) Resin pretreatment: first soak 24h with 95% ethanol, remove the particles with poor quality floating on the liquid surface, then, soak 24h with 0.2mol / LHCl and 4% NaOH solution respectively, to remove resin Various organic substances mixed in the synthesis process were finally washed with deionized water until neutral for later use;

[0077] (2) 1000 g of donkey-hide gelatin is added with 8 times the amount of ultra-pure water to melt the gelatin to prepare a donkey-hide gelatin solution;

[0078] (3) Adjust the pH of donkey-hide gelatin solution to 8.5, add 0.1% nattokinase by total weight, hydrolyze at 37°C for 3h, and treat at 100°C for 15min;

[0079] (4) Adjust the pH of the donkey-hide gelatin solution after enzymolysis to 4.0, add 0.3% Aspergillus niger acid protease by total weight, hydrolyze at 33°C for 4h, and treat at 100°C for 15min;

[0080] (5) filteri...

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Abstract

The invention discloses colla corii asini low-molecule peptide as well as a preparation method and application thereof, and belongs to the technical field of colla corii asini preparations. The collacorii asini low-molecule peptide is prepared by hydrolyzing colla corii asini with proteases and removing bitterness, wherein the proteases are natto kinase and acid protease. The colla corii asini low-molecule peptide prepared by the method is high in low-molecule peptide and high in biological availability. Adsorption is adopted with macroporous resins, so that the bitter components in the low-molecule peptide can be adsorbed, and the added plant extracts can regulate the bitterness and the fishy smell of the colla corii asini low-molecule peptide; and the added plant extracts are matched with stachyose, jujube vinegar and neohesperidin dihydrochalcone, so that the prepared colla corii asini low-molecule peptide preparation is moderate in sour and sweet degrees and free from fishy smelland bitterness. In addition, the colla corii asini low-molecule peptide can also improve the function of the stomach and the intestine, and prevent indigestion caused by taking of the colla corii asini.

Description

technical field [0001] The invention belongs to the technical field of donkey-hide gelatin preparations, and in particular relates to a low-molecular-weight peptide of donkey-hide gelatin, its preparation method and application. Background technique [0002] Donkey-hide gelatin is a traditional top-grade nourishing and blood-enriching medicine. It tastes sweet and flat. It enters the lung, liver, and kidney meridians. , Anti-fatigue, improve immunity, suitable for a wide range of people. [0003] First of all, the newly made donkey-hide gelatin always contains some fire poison, so taking fresh donkey-hide gelatin will cause hyperactivity of anger and various poisoning symptoms; secondly, donkey-hide gelatin is greasy in nature, and people with spleen and stomach deficiency are likely to cause indigestion symptoms; In addition, most pure donkey-hide gelatin has a special fishy smell, which is difficult to be accepted by consumers, which affects the use and promotion of donke...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/18A23L33/105A23L5/20A23L33/00
CPCA23V2002/00A23L5/273A23L33/00A23L33/105A23L33/18A23V2300/44A23V2300/14
Inventor 段晓菲
Owner 青岛霍中医生物科技有限公司
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