The invention discloses a fresh and sweet high-power compound sweetening agent and a preparation process and application thereof, and belongs to the field of food additives, neohesperidin dihydrochalcone, disodium nucleotide (I + G), vitamin and yeast extract endow the sweetening agent with fresh and sweet flavor. The sweetening agent mainly comprises the following components: miraculin, citric acid, monopotassium glycyrrhizinate, ammonium glycyrrhizinate, tripotassium glycyrrhizinate, stevioside, mogroside, sucralose, neotame, neohesperidin dihydrochalcone, xylitol, erythritol, a yeast extract, disodium nucleotide (I + G), edible salt, vitamins and maltodextrin. The invention also discloses a preparation process of the fresh and sweet high-power compound sweetening agent, the process is simple, convenient and rapid, the sweetening agent can be quickly put into production, the sweetening agent can be applied to food, soy sauce, beverages, low-alcohol wine, dairy products or bakery products, and the sweetening agent can be prepared through scientific and reasonable compounding. Therefore, the obtained sweetening agent is prominent in fresh and sweet flavor, high in solubility, good in stability, high in safety and low in sweetness cost.