Neohesperidin dihydrochalcone obtained from whin plants

A new hesperidin dihydrochalcone and Oxytropis technology, which is applied in the field of medicine, can solve the problems of less plant research and blank research on the biological activity of dihydrochalcone, and achieve the effect of improving comprehensive utilization

Inactive Publication Date: 2017-09-22
折改梅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the research on the phytochemical components of Oxytropis genus is mainly concentrated on a few plants such as Oxytropis florida, and there are few studies on some other plants that are commonly used by folks.
And the research on the flavonoid components of Oxytropis genus plants such as Oxytropis multi-leaf is mostly focused on the flavonoids, flavonol components and their pharmacological activities, but the research on the dihydrochalcone components and their biological activities is basically blank.

Method used

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  • Neohesperidin dihydrochalcone obtained from whin plants
  • Neohesperidin dihydrochalcone obtained from whin plants
  • Neohesperidin dihydrochalcone obtained from whin plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Preparation of neohesperidin dihydrochalcone in Oxytropis multileaf

[0048] Take 1.0 kg of Oxytropis multi-leaf medicinal material, and ultrasonically extract 3 times with 20 times the amount of ethanol with a mass fraction of 70%, 1 hour each time. Filter the extract from the previous step, reduce the pressure to 0.07 MPa, recover ethanol, and obtain 187 g of extract. The content of neohesperidin dihydrochalcone in the extract is 4.3%. Take extractum 20 g and dissolve in water and be mixed with a solution of 15% by weight, filter to obtain the upper column liquid; this upper column liquid is passed through the AB-8 macroporous adsorption resin (400 g) column, after washing with 2 Bv of 20% ethanol , discard the eluate; use 50% ethanol to elute with a flow rate of 1 Bv / hr, and collect the eluate; use high-efficiency liquid relative to the enrichment rate and purity of neohesperidin dihydrochalcone in the eluate Detected; after enrichment, the content of ne...

Embodiment 2

[0049] Embodiment 2: Preparation of neohesperidin dihydrochalcone in Oxytropis multileaf

[0050] Take 1.0 kg of Oxytropis multi-leaf medicinal material, warm soak and extract with 10 times the amount of 60% ethanol for 3 times, 1 hour each time. Filter the extract from the previous step, reduce the pressure to 0.08 MPa, recover ethanol, and obtain about 172 g of extract. The content of neohesperidin dihydrochalcone in the extract is 4.1%. Dissolve the extract in water, add sodium hydroxide solution, and adjust the pH to 8. Filtrate, take the filtrate, add sulfuric acid solution to the filtrate, adjust the pH to 3, centrifuge after standing at room temperature, collect the precipitate, and dry it in vacuum to obtain 119 g of extract. After the extract was dissolved with 60% ethanol, the enrichment rate and purity of neohesperidin dihydrochalcone in the solution were detected by high performance liquid chromatography; after enrichment, neohesperidin dihydrochalcone Ketone is...

Embodiment 3

[0051] Example 3: Preparation of neohesperidin dihydrochalcone in Oxytropis multileaf

[0052] Take 1.0 kg of Oxytropis multi-leaf medicinal material, and reflux extraction with 20 times the amount of 80% methanol for 3 times, each time for 1.5 hours. Filter the extract from the previous step, reduce the pressure to 0.09 MPa, recover methanol, and obtain about 181 g of extract. The content of neohesperidin dihydrochalcone in the extract is 3.9%. Take extractum 20 g and dissolve in water and be mixed with a solution of 15% by weight, filter to obtain the upper column liquid; this upper column liquid is passed through the HPD-722 macroporous adsorption resin (600 g) column, after washing with 2 Bv of 25% methanol , discard the eluate; use 40% methanol, elute with a flow rate of 1 Bv / hr, collect the eluate; use high-efficiency liquid relative to the enrichment rate and purity of neohesperidin dihydrochalcone in the eluate Detect; after the enrichment, the content of neohesperid...

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Abstract

The present application relates to a new hesperidin dihydrochalcone obtained from plants of the genus Oxytropis of the family Fabaceae. Beans, Oxytropis sativa, Oxytropis floret, Oxytropis falciformum, and Oxytropis gansu are prepared by solvent extraction, macroporous resin purification or acid-base purification, and recrystallization. Neohesperidin dihydrochalcone is an excellent medicinal sweetener and taste-screening agent, and has various pharmacological activities such as anti-oxidation and hypoglycemia. In the present invention, Oxytropin is used as the raw material for extracting neohesperidin dihydrochalcone for the first time. The source of the raw material is abundant and the preparation process is simple, which not only meets the market demand of neohesperidin dihydrochalcone, but also Can make full use of Oxytropis plant resources, improve its comprehensive utilization.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to an extract neohesperidin dihydrochalcone, in particular to a neohesperidin dihydrochalcone extracted from plants of the genus Oxytropis genus Fabaceae. Background technique [0002] Fabaceae ( Leguminosae ) Papilionaceae Oxytropis plants, mostly herbs, semi-shrubs or dwarf shrubs, more than 300 species in the world, mainly distributed in the temperate and frigid regions of the northern hemisphere and their arid and alpine regions. There are more than 150 species of Oxytropis in my country, mainly including Oxytropis multileaf ( Oxytropis myriophylla ), Oxytropis ( Oxytropis ramosissima ), Oxytropis ( Oxytropis psammocharis ), Oxytropis microflora ( Oxytropis glabra ), Oxytropis falciparum ( Oxytropis falcata Bunge ), Gansu Oxytropis ( Oxytropis kansuensis Bunge ), etc., roughly distributed in Northwest, North China, Northeast, Southwest and other places. It is bit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/203C07H1/08
CPCC07H15/203C07H1/08
Inventor 折改梅赵怡程刘斌王炎田玉欣陈晓怡白少娟
Owner 折改梅
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