A method for in-lab breeding of Spodoptera frugiperda in winter comprises the steps of (S1) adult pairing, (S2) uniform egg cultivation, (S3) incubation and feeding, (S4) excrement cleaning and feed changing for first to third larvae, (S5) dividing and breeding of advanced-age larvae, (S6) preparation of a pupation environment, and (S7) centralized pupa breeding and worm formation by pupation. Different devices are used in different stages, disposable plastic cups are used during adult pairing and larva breeding, narrow space of the disposable plastic cups is beneficial for adult pairing, andescape of young-age larvae which are small in size and quick in creeping can be prevented through cup breeding; and a latticed breeding device is used for the advanced-age larva, so that mutual bitingcaused by competition can be avoided, space is saved, and the larvae of different ages can be bred at the same time. The method for in-lab breeding of Spodoptera frugiperda in winter effectively increases a survival rate, is high in breeding density and small in floor area, increases the yield, is convenient to operate and saves time and effort.