Detection method for sunscreen efficacy of sunscreen skin care products
A detection method and technology of skin care products, applied in the detection field, can solve the problems of unsuitability for a large amount of foreign substances, long experiment period, high cost, etc., and achieve scientific and effective sun protection effect, short experiment period, and easy breeding
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[0031] A detection method for sunscreen efficacy of sunscreen skin care products, the specific experimental method is:
[0032] (1) Before the experiment (approximately 22:00 every night), a pair of male and female zebrafish (wild-type AB line) was selected in the mating box, and the male and female zebrafish were separated with a partition, and placed in the night (dark) state for 10 hours.
[0033] (2) The next morning (around 8:00) remove the partition, and the zebrafish mate and lay eggs.
[0034] (3) Fish out the zebrafish and collect zebrafish eggs.
[0035] (4) Take the time of pulling out the partition as 0hpf, and start the experiment at 72hpf.
[0036] (5) The experimental solution was prepared, the aquaculture water group without ultraviolet irradiation was -UV, and the aquaculture water group was +UV after ultraviolet irradiation.
[0037] (6) Load the zebrafish on a glass plate, and wash it 2 to 3 times with cultured water (or PBS). Each zebrafish was numbered ...
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