Silk fibroin microneedle electrode and preparation and application thereof

A silk fibroin microneedle and silk fibroin technology, which is applied to silk fibroin microneedle electrodes and their preparation and application fields, can solve the problems of easy shedding, easy occurrence of allergic reactions, etc. Less sensitizing effect

Active Publication Date: 2019-08-16
NANTONG TEXTILE & SILK IND TECH RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conductive film deposited on the surface of the electrode substrate by this method is easy to fall off, and the human body and stainless steel microneedles are prone to allergic reactions.

Method used

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  • Silk fibroin microneedle electrode and preparation and application thereof
  • Silk fibroin microneedle electrode and preparation and application thereof
  • Silk fibroin microneedle electrode and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] see Figure 1-2 , a silk fibroin microneedle electrode for detecting glucose concentration of the present invention, comprising a silk fibroin microneedle body and a platinum wire electrode part 3 .

[0041] The silk fibroin microneedle body includes a base 10 and a plurality of solid needles 11 uniformly planted on one side of the base 10 . The base 10 is cylindrical, and the height of the solid needle body 11 is 400-600 μm. The silk fibroin microneedles have high hardness in the dry state, but form a hydrogel after absorbing body fluids, and a number of micropores for glucose to pass are evenly distributed in the hydrogel. The components of the silk fibroin microneedle body include silk fibroin, polyols and glucose oxidase 2. One or more of sorbitol, erythritol and xylitol can be selected as the polyol. The body of silk fibroin microneedles becomes transparent after absorbing body fluid and swelling.

[0042] The platinum wire electrode part 3 extends out of the b...

Embodiment 2

[0047] A kind of preparation method of the silk fibroin microneedle electrode that is used to detect glucose concentration of the present invention comprises the following steps:

[0048] (1) Preparation of silk fibroin solution: take 80g of silkworm raw silk and put it into 4000mL of 0.06% sodium carbonate solution, boil it three times at 98-100°C, use deionized water for each of the three times, and treat it for 30 minutes each time to remove the raw silk The sericin in the fiber is washed, loosened and then dried in an oven at 60°C to obtain pure silk fiber. Dissolve the dried silk fiber in 9.3M lithium bromide solution at 60°C, the bath ratio is 3:20, and the dissolution time is about 1h. After cooling, take it out and put it into a dialysis bag and dialyze it in deionized water for 3d. Filtrate with absorbent cotton to obtain pure silk fibroin solution, concentrate it at room temperature, and put it in a refrigerator at 4°C for later use.

[0049] (2) Platinum wire treat...

Embodiment 3

[0056] Silk fibroin microneedle electrodes were prepared according to the method of Example 2, the difference being:

[0057] In step (2), the diameter of the platinum wire is 0.008 mm, and the soaking time is 2 minutes.

[0058] In step (3), the concentration of the pure silk fibroin solution is 50 mg / mL, the volume ratio of the pure silk fibroin solution to sorbitol aqueous solution is 5:0.75, and the concentration of glucose oxidase in the mixed solution is 6 mg / mL.

[0059] In step (6), the microneedle mold inserted with the platinum wire was placed in a constant temperature and humidity environment (300K temperature, 60% relative humidity) to dry and balance for 17 hours.

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Abstract

The present invention relates to a silk fibroin microneedle electrode, which comprises a silk fibroin microneedle body and a platinum wire electrode portion, the silk fibroin microneedle body comprises a substrate and a plurality of solid needle bodies uniformly implanted on one side surface of the substrate, the silk fibroin microneedle has high hardness in a dry state but forms a plurality of pores for the passage of glucose after absorbing the liquid, and the components of the silk fibroin microneedle body include silk fibroin, polyhydric alcohol and glucose oxidase; the platinum wire electrode portion extends from the inside of the solid needle body away from the solid needle body, and the platinum wire electrode portion includes a platinum wire body, a catalyst layer and a carrier layer which are sequentially disposed from the inside to the outside; The catalyst layer is a glucose oxidase layer and the carrier layer is a silk fibroin layer. The preparation method has the advantages of simple preparation method, low cost, wide detection range, good biocompatibility and high electrode stability.

Description

technical field [0001] The invention relates to the technical field of enzyme biosensors, in particular to a silk fibroin microneedle electrode and its preparation and application. Background technique [0002] In recent decades, blood glucose detection technology has developed rapidly, and more fast and safe detection technologies have been proposed and applied. Although the detection accuracy of the minimally invasive blood glucose meter is not as high as that of venous blood, it plays an important role in daily blood glucose management at home because of its convenience and ease of use. However, repeated traumatic testing puts patients under physical and mental pressure, which greatly affects the execution of blood sugar monitoring and management, which in turn causes large fluctuations in blood sugar levels of patients, and cannot be tested for a long time, making it difficult to achieve 24 hour monitoring. [0003] In recent years, most of the production of blood gluc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B5/1473A61B5/1486
CPCA61B5/14532A61B5/1473A61B5/1486
Inventor 卢神州赵亮姜福建温壮壮朱明枚
Owner NANTONG TEXTILE & SILK IND TECH RES INST
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