Method for comprehensively utilizing euphausia superba shells

An Antarctic krill and astaxanthin technology, applied in the application, fish protein composition, food science and other directions, can solve the problems of non-recycling of protein, low quality of chitin products, and large amount of acid and alkali. The effect of realizing sustainable economic development, promoting technological transformation and technological level, and improving utilization rate

Inactive Publication Date: 2014-11-26
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, domestic chitin is mainly processed by chemical methods, which use a large amount of acid and alkali, and protein is usually not recycled, resulting in waste of resources and easy to cause serious environmental pollution; The product quality is not high and fluctuates greatly

Method used

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  • Method for comprehensively utilizing euphausia superba shells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The raw material is frozen Antarctic krill shells. The shrimp shells are added to water according to the shrimp shell / water mass ratio of 1:4, heated to 50°C, kept warm, and stirred in the dark for 2 hours. Adjust the pH to 8.0, add alkaline protease for enzymolysis, the dosage is 3000U / g protein according to the enzyme activity, and stir for 1.5h in the dark, and filter with a 60-mesh filter after the reaction. The filtrate was centrifuged at 4000rpm for 15min, and the supernatant was separated from the precipitate. The supernatant was concentrated in vacuum until the soluble solids were 35% and then spray-dried to obtain protein powder with a protein content of 85%. The centrifuged precipitate was freeze-dried to obtain protein powder rich in astaxanthin, wherein the protein content was 50%, and the astaxanthin content was 900 mg / kg. The remaining residue after filtration is used to prepare chitin.

Embodiment 2

[0018] Frozen Antarctic krill shells are used as raw materials, and the Antarctic krill shells are added to water at a mass ratio of Antarctic krill shells / water of 1:6, heated to 60°C, kept warm, and stirred for 1.5 hours in the dark. Adjust the pH to 8.0, add alkaline protease for enzymolysis, the dosage is 1500U / g protein according to the enzyme activity, keep away from light and stir for 2.5h, filter with a 60-mesh filter after the reaction, and centrifuge the enzymolysis solution at 5000rpm After 15 min, the supernatant was separated from the precipitate. The supernatant was concentrated in vacuum until the soluble solids were 32% and then spray-dried to obtain protein powder with a protein content of 82%. The centrifugal precipitate was freeze-dried to obtain protein powder rich in astaxanthin, wherein the protein content was 55%, and the astaxanthin content was 750 mg / kg. The remaining residue after filtration is used to prepare chitin.

Embodiment 3

[0020] The raw material is frozen Antarctic krill processing scraps, and the scraps are added to water according to the ratio of scraps / water mass of 1:5, heated to 55°C, kept warm, and stirred for 1 hour in the dark. Adjust the pH to 8.5, add alkaline protease for enzymolysis, the dosage is 2000U / g protein according to the enzyme activity, and stir for 2 hours in the dark, and filter with a 60-mesh filter after the reaction. The filtrate was centrifuged at 3000 rpm for 15 min, and the supernatant was separated from the precipitate. The supernatant was concentrated in vacuum until the soluble solids were 30% and then spray-dried to obtain protein powder with a protein content of 75%. The centrifugal precipitate was freeze-dried to obtain protein powder rich in astaxanthin, wherein the protein content was 60%, and the astaxanthin content was 600mg / kg. Other operations are the same as in Example 1. The remaining residue after filtration is used to prepare chitin.

[0021] Com...

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Abstract

The invention discloses a method for comprehensively utilizing euphausia superba shells. The method is a comprehensive utilizing method, wherein the shells are hydrolyzed by means of endogenous proteinases and exogenous alkaline proteinases of euphausia superba with high activity based on euphausia superba shells as raw materials to recover proteins and astaxanthin, while residues are used for producing chitin. The shells are hydrolyzed by means of endogenous proteinases and exogenous alkaline proteinases, so that the protein recovery rate is effectively increased. Enzymatic hydrolysates are filtered and centrifugalized, and the filtrate is concentrated to obtain protein concentrate or sprayed and dried to obtain soluble protein powder. The protein content in the protein powder is 75-85%. Precipitates are freezed and dried to obtain the protein powder rich in astaxanthin, wherein the protein content is 50-60% while the astaxanthin content is 600-900mg / kg. The addition amount of exogenous enzymes is reduced by 40-50% by replacing part of exogenous enzymes by enzymolysis of endogenous proteinases in a way of combining the characteristic that the endogenous proteinases of the euphausia superba are high in activity, so that the cost is greatly lowered.

Description

technical field [0001] The invention provides a method for comprehensively utilizing Antarctic krill shells, belonging to the field of food biotechnology. Background technique [0002] Antarctic krill belongs to the orders of Arthropods, Crustacea, and Krill, and is the largest single biological resource on earth. There are 7 to 8 species of krill living in the Antarctic waters, and the general guide for Antarctic krill available for development is Euphausia superb. Krill is unique in many ways. It is one of the most resource-rich multicellular animals in the world. It is also the strongest enzyme producer found to decompose proteins. It is currently one of the animals with the largest concentration of marine organisms on the earth. Antarctic krill is one of the largest single species of biological resources on the planet. The annual catch is equivalent to the sum of the current annual fish and crustacean catches in the world. It is considered to be the largest and last ani...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23J1/04
Inventor 夏文水姜启兴李姝婧刘建君于沛沛刘富俊许艳顺许学勤许兆滨
Owner JIANGNAN UNIV
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