Method for establishing Macaca fascicularis experimental autoimmune encephalomyelitis model and application thereof

A technology of cerebrospinal meningitis and autoimmunity, which is applied in the establishment of cynomolgus monkey experimental autoimmune cerebrospinal meningitis model, can solve the problems of long induction period, large rhesus monkeys, and obstacles to the application of the model, and achieve animal The effect of small individual, reduction of human factors, and strict quality control

Inactive Publication Date: 2014-07-23
上海浦灵生物科技有限公司
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  • Abstract
  • Description
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Problems solved by technology

However, the disadvantages of this method are: 1. The induction period is too long; 2. The relatively subjective disease symptom scoring standard is adopted; 3. The rhesus macaque is relatively large
These shortcomings hinder the application of the model in scientific research, drug development, and industrialization practice

Method used

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  • Method for establishing Macaca fascicularis experimental autoimmune encephalomyelitis model and application thereof
  • Method for establishing Macaca fascicularis experimental autoimmune encephalomyelitis model and application thereof
  • Method for establishing Macaca fascicularis experimental autoimmune encephalomyelitis model and application thereof

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Embodiment Construction

[0035] In order to enable those skilled in the art to understand the present invention more comprehensively, the present invention is further described below in conjunction with the examples, but the present invention is not limited in any way.

[0036] Experimental animals: 4 cynomolgus monkeys (Macaca fascicularis, male, 4 years old, weighing 3-5 kg), purchased from Hainan Jingang Experimental Animal Technology Co., Ltd.

[0037] Main reagents: Complete Freund adjuvant (Complete Freund adjuvant, CFA), purchased from Sigma Company; MOG34-56 purchased from Jill Biochemical (Shanghai) Co., Ltd.

[0038] Preparation of the main solution: MOG34-56 was dissolved in physiological saline, and emulsified with an equal volume of CFA for 5 minutes at 4°C.

[0039] Main instruments: magnetic resonance apparatus (GE), 1.5T.

[0040] experimental method:

[0041] The prepared MOG-CFA emulsion was injected intradermally at ten points, 1ml per monkey.

[0042] From 1 day after the injec...

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Abstract

The invention discloses a method for establishing a Macaca fascicularis experimental autoimmune encephalomyelitis model and application thereof. According to a concrete technical scheme in the invention, the method comprises the following steps: preparing an emulsion (an MOG solution: CFA = 1: 1) from MOG 34-56 (100 mu g/ml); narcotizing Macaca fascicularis for experiments and injecting 1 ml of the prepared emulsion at 10 injection points; carrying out immunization injection of the emulsion (secondary immunization) according to the above-mentioned method and dose in 7 days after the primary immunization injection (primary immunization); carrying out clinical observation in one day after primary immunization and recording clinical scores; and determining pathogenic sites, degrees and the like by using an MRI iconographic method at pathogenic time nodes. The model established in the invention has an application value which cannot be achieved by other rodent models, has the characteristics of recurrence-alleviation type attacks, low cost, wide availability of Macaca fascicularis for experiments and the like compared with a Macaca rhesus model and has a wide application scope in fields related to multiple sclerosis diseases.

Description

technical field [0001] The invention relates to the establishment of a disease model, in particular to a method for establishing a cynomolgus monkey experimental autoimmune cerebrospinal meningitis model and its application. Background technique [0002] Experimental autoimmune encephalomyelitis (EAE) is induced by isotype, allotype, and xenotype-induced encephalitic antigens, produced by experimentally sensitive animals, mediated by cellular immune responses, and central nervous system Delayed allergic autoimmune disease characterized by central nervous system (CNS) white matter demyelination, its immune pathogenesis and lesions are very similar to human multiple sclerosis (multiple sclerosis, MS), it is recognized as a research Ideal model for multiple sclerosis disease. [0003] Current experimental autoimmune encephalomyelitis (EAE) models are usually established in rodents. Because rodents are far away from humans in evolutionary relationship, practice has shown that ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/17A61K9/107
Inventor 倪佳王松邓继林刘俊娥张红宋之战
Owner 上海浦灵生物科技有限公司
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