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Preparation method for deer blood active composition

A technology for active components and deer blood, which is applied in the field of preparation of deer blood active components, can solve the problems of restricting the consumption of deer blood and the like, achieve good application prospects, expand the applicable population, and be easily accepted.

Active Publication Date: 2016-02-10
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In ancient my country, as early as the Song Dynasty, the nobles of the palace raised deer blood for raw drinks. However, this way of eating limited the number of people who can eat deer blood. It is urgent to develop deer blood health products that are convenient to eat and have clear effects, so that deer blood, a royal nourishment Products into the families of ordinary people

Method used

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  • Preparation method for deer blood active composition
  • Preparation method for deer blood active composition
  • Preparation method for deer blood active composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Preparation of Active Components of Deer Blood

[0020] Take 10 liters and add 20 grams of anticoagulant EDTA-K.2H 2 O fresh red deer deer blood, add 10 liters of deionized water, mix well, heat to 80°C, stir and keep warm for 10 minutes, cool down to 40°C, adjust the pH to 2.0 with 1M HCl, add 0.2 g of pepsin, and stir for 24 hours to enzymatically hydrolyze; After the solution is completed, heat up to 80°C, stir and keep warm for 10 minutes, then cool down to 40°C, add 1 gram of brewing active dry yeast, and ferment for 24 hours; after the fermentation, heat up to 100°C, keep warm for 10 minutes; after the reaction, freeze-dry to obtain powder The active ingredient of deer blood.

[0021] 2. Antioxidant capacity of active components of deer blood

[0022] 2.1 Determination of antioxidant activity

[0023] 2.1.1 Determination of iron reducing ability

[0024] Fe 3+ -Tripyridine-triazine (TPTZ) can be reduced to Fe by reducing species in the sample 2+ It is blu...

Embodiment 2

[0055] Take 100 liters of fresh sika deer blood with 15000U anticoagulant heparin, add 100 liters of deionized water, mix well, heat to 100°C, stir and keep warm for 10 minutes; cool down to 50°C, adjust the pH to 8.0 with 1M NaOH, add 500 grams of alkali Protease, stirring for 5 hours; after enzymolysis, heat up to 80°C and stir for 10 minutes, then cool down to 30°C, add 500 grams of brewing active dry yeast, and ferment for 12 hours; after fermentation, heat up to 100°C, keep warm 10 minutes; freeze-drying after completion of the reaction to obtain powdered deer blood active components.

[0056] The activity detection result of the active component of deer blood is the same as that of Example 1.

Embodiment 3

[0058] Take 10 liters of fresh sika deer antler blood with 20 grams of anticoagulant sodium oxalate, add 10 liters of deionized water, mix evenly, heat to 60°C, stir and keep warm for 60 minutes, cool down to 55°C, adjust the pH to 8.0 with 1M NaOH, add 2 Bromelain and papain mixture (the mass ratio of the two is 1:1), stirred for 10 hours; after the end of the enzymolysis, the temperature was raised to 80°C, stirred and kept for 10 minutes, then cooled to 45°C, and 20 grams of wine-making activity was added. The yeast is fermented for 8 hours; after the fermentation is completed, the temperature is raised to 100° C., and the temperature is kept for 10 minutes; after the reaction is completed, the powdered deer blood active component is obtained by freeze-drying.

[0059] The activity detection result of the active component of deer blood is the same as that of Example 1.

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Abstract

The invention relates to a preparation method for a deer blood active composition. The deer blood active composition is obtained by taking fresh deer blood as a raw material and performing virus inactivation, protease first enzymatic hydrolysis, saccharomyces cerevisiae secondary fermentation and drying. The deer blood active composition prepared according to the technology possesses the following characteristics: (1) large-molecular-weight protein is degraded into small molecules in the enzymatic hydrolysis process, deer blood is prevented from causing adverse reactions such as allergy, and because the peptide active site is exposed, the deer blood active composition possesses ACE inhibition activity, DPP-IV inhibition activity, antibacterial activity and other functions; and (2) after the deer blood active composition subjected to first enzymatic hydrolysis and secondary fermentation is decomposed, original fishy smell of a blood product is eliminated, and the taste is relatively easily accepted. The deer blood active composition is applicable to fields of medicines, functional food, food additives and the like, and possesses wide application prospect.

Description

technical field [0001] The invention relates to the preparation technology of active components of deer blood. Background technique [0002] Deer blood has been a precious tonic since ancient times. Chinese herbal books such as "Compendium of Materia Medica" and "New Edition of Materia Medica" have records of the medicinal value of deer blood. The use of deer blood for fitness was first seen in the "Qingbo Magazine" in the Song Dynasty. The "Compendium of Materia Medica" written by Li Shizhen in the Ming Dynasty recorded the medical effects of deer blood in detail: "mainly impotence, tonic, lumbago, falls, rabies, and Take alcohol to control lung impotence, hematemesis, and collapse, and those who are suffering from qi pain and want to be in danger drink it to heal immediately. It can greatly tonify deficiency and damage, benefit essence and blood, relieve acne poison, and drug poison. In ancient my country, as early as the Song Dynasty, the royal palace and nobles used it Br...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/14A23L1/29A61P39/06A61P37/04A61P35/00A61P9/12
Inventor 邹汉法靳艳
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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