Culture method for inducing DC-CIK amplification

A technology of DC-CIK and PCD-DC-CIK, which is applied in the field of culture to induce DC-CIK expansion, can solve the problems of high technical requirements for acquisition and culture, low tumor specificity, and long culture time, etc., and achieve tumor proportion Decrease, increase proliferation and phagocytosis, and prolong survival

Pending Publication Date: 2020-10-02
湖南科诺康美生命科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, ALL and CIK in vitro culture takes a long time, the method is cumbersome, and the tumor specificity is not strong; while the expansion of TIL or CTL in lymphoid tissue requires high technical requirements for material acquisition and culture, which is not easy to achieve

Method used

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  • Culture method for inducing DC-CIK amplification
  • Culture method for inducing DC-CIK amplification
  • Culture method for inducing DC-CIK amplification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] refer to Figure 1-4 , a method for inducing DC-CIK with platycodon saponin D, comprising the following steps:

[0029] S1. Separation and purification of Platycodon saponin D. Platycodon saponin D is extracted with ethanol from Platycodon grandiflora, extracted, separated by silica gel, and chromatographically separated to be used. PCD-DC-CIK induction culture containing platycodon saponin D, the concentration of platycodon saponin D is 40 μg / mL;

[0030] S2, the preparation of PCD-DC-CIK cells, including the following steps,

[0031] 1) Patient preparation: Patients who choose this treatment method according to the indications and contraindications of immune cell PCD-DC-CIK treatment, sign an informed consent form, indicate precautions, and perform blood routine tests on all patients;

[0032] 2) Peripheral blood collection: the sterile tube contains heparin sodium, and 50 mL of peripheral blood from tumor patients is extracted intravenously into a sterile tube and...

Embodiment 2

[0043] refer to Figure 1-4 , a method for inducing DC-CIK with platycodon saponin D, comprising the following steps:

[0044] S1. Separation and purification of Platycodon saponin D. Platycodon saponin D is extracted with ethanol from Platycodon grandiflora, extracted and separated by silica gel, and chromatographically separated to be used. PCD-DC-CIK induction culture containing platycodon saponin D has a concentration of 60 μg. / mL;

[0045] S2, the preparation of PCD-DC-CIK cells, including the following steps,

[0046] 1) Patient preparation: Patients who choose this treatment method according to the indications and contraindications of immune cell PCD-DC-CIK treatment, sign an informed consent form, indicate precautions, and perform blood routine tests on all patients;

[0047] 2) Peripheral blood collection: the sterile tube contains heparin sodium, and 50 mL of peripheral blood from tumor patients is extracted intravenously into a sterile tube and mixed well. The so...

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Abstract

The invention relates to the technical field of culture methods, in particular to a culture method for inducing DC-CIK amplification. The method comprises the following steps: separation and purification of platycodin D, preparation of PCD-DC-CIK cells, feedback of immune cells PCD-DC-CIK, and monitoring on growth characteristics of platycodin D induced PCD-DC-CIK cells and conventionally inducedDC-CIK cells, and evaluating of lifetime of PCD-DC-CIK cells of a tumor-bearing mouse before and after treatment. Platycodin D is utilized to effectively increase proliferation and phagocytosis capacities of macrophages, so that the respective functions of DC and CIK cells are synergistically exerted; in-vitro experiments prove that a proliferation rate of the PCD-DC-CIK cells induced and culturedby the method is remarkably improved compared with conventional induction, and tumor inhibition activity is remarkably enhanced; and animal experiments show that after the tumor-bearing mouse is subjected to treatment by the method, the tumor proportion is obviously reduced, and the lifetime is obviously prolonged.

Description

technical field [0001] The invention relates to the technical field of culture methods, in particular to a culture method for inducing DC-CIK expansion. Background technique [0002] Autologous immune cell therapy refers to the isolation of mononuclear cells (Peripheral blood mononuclear cells, PBMCs) from the patient's own peripheral blood, which are activated and expanded in vitro and then reinfused into the patient's body so that they can kill tumors or other abnormal cells, and regulate and Enhance the immune function of the body. Mainly include (1) Cytokine-induced killer cell (CIK) therapy. It is a group of heterogeneous tumor cell killing activity obtained after stimulating PBMC with interleukin-2 (Interleucin-2, IL-2), γ-type interferon (Interferon-, IFN-γ), CD3 monoclonal antibody, etc. (2) Autologous activated lymphocyte (AAL) therapy. Lymphokine-activated killer cells (Lymphokine-activated killer cells, LAK) were mainly used to activate lymphocytes in PBMCs wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783C12N5/0784
CPCC12N5/0638C12N5/0636C12N5/0639C12N2500/30
Inventor 吴金芸雷登黄春兰
Owner 湖南科诺康美生命科技有限公司
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