124 results about "Animal study" patented technology

The invention relates to the technical field of data management and information systems, in particular to an experimental data management system conforming to GLP specifications, which comprises a data acquisition and standardization unit, a data storage and version management unit, a natural language query and interaction unit, a data quality and compliance verification unit and an audit tracking and authority management unit. According to the method, professional terms and query intentions are accurately analyzed by means of a field-specific language model, intelligent recognition and visual interaction of image data are realized by the multi-modal interaction module, dialogue continuity is maintained by the context dialogue management module, and an association analysis scheme is actively pushed by the intelligent recommendation module. Intelligent retrieval and analysis requirements of animal experiment data are efficiently supported, the compliance and quality of the experiment data are guaranteed, and data risks are effectively identified and handled.

The invention relates to a radionuclide labeled trimer probe 68Ga-TRAP-(FAPI) 3 targeting FAP and a preparation method thereof, Trap is used as a trivalent chelating platform, and three FAPI targeting units are covalently connected by clicking a chemical linking arm to form a trimer structure; the invention further relates to application of the 68Ga-TRAP-(FAPI) 3 probe in preparation of imaging agents or therapeutic drugs for diagnosing FAP positive diseases, experiments prove that the trimer probe is good in affinity and high in stability, and in cell experiments and animal experiments, the uptake rate of FAP positive cells is kept at a high level within 240 minutes; preclinical and preliminary clinical applications show that the tumor detection rate of the < 68 > Ga-TRAP-FAPI 3 in various cancer models is superior to that of < 18 > F-FDG, and compared with < 68 > Ga-FAPI-04, the < 68 > Ga-TRAP-FAPI 3 is clearer to display focuses (particularly tiny metastases), higher in uptake intensity and particularly outstanding in delayed imaging, and the < 68 > Ga-TRAP-FAPI 3 can be used for preparing a medicine for treating cancer. And a novel drug candidate with better performance is provided for precise diagnosis and staging of tumors and subsequent radionuclide targeted therapy.

The invention discloses armillariella mycelium polysaccharide ATMP capable of improving cognitive impairment and a preparation method and application of the armillariella mycelium polysaccharide ATMP. The polysaccharide is composed of two components, and the molecular weights of the two components are 2.30 * 10 < 4 > Da and 4.56 * 10 < 5 > Da respectively; the monosaccharide composition comprises mannose (Man), glucose (Glc) and galactose (Gal), and the molar ratio of the mannose (Man) to the glucose (Glc) to the galactose (Gal) is 1: 16.64: 1.96. Animal experiment results show that the armillariella mycelium polysaccharide ATMP can improve cognitive impairment induced by lipopolysaccharide (LPS) by improving the spatial memory ability of mice, repairing the disorder state and deletion injury of neurons in a hippocampal region and relieving neuroinflammatory response, so that the obvious neuroprotective effect is achieved. As a natural brain-benefiting active component, the armillariella tabescens mycelium polysaccharide ATMP has a wide application prospect in the related fields of brain health.

The application discloses a method for establishing a mouse model of MHC haplotype compatible allogeneic hematopoietic cell transplantation, and belongs to the technical field of animal experiment model construction. The technical problem to be solved by the application is how to construct a mouse model which can be used to study the immune reconstruction mechanism and treatment effect evaluation of MHC haplotype compatibility and MHC full compatibility in bone marrow cell transplantation recipient mice. To solve the technical problem, the application provides a method for constructing a mouse model of MHC haplotype compatible allogeneic hematopoietic cell transplantation, which comprises transplanting bone marrow cells of a donor mouse into a recipient mouse, and the MHC haplotype of the donor mouse and the recipient mouse is compatible. The application sets a modeling condition, and the model establishment condition is evaluated by survival condition, graft-versus-host disease and rejection reaction observation, flow cytometry and histopathological section, so as to prove that the model is reliable and stable.

The invention provides a liquid phase tandem time-of-flight mass spectrometry detection method for the content of tirofiban in liver tissue, and belongs to the technical field of detection. According to the present invention, the concentration of tirofiban in the liver tissue is detected through the liquid phase tandem time-of-flight mass spectrometry, such that the characteristics of low detection limit, immediate analysis, high sensitivity, good repeatability, good indoor reproducibility and high accuracy are provided, the matrix effect is substantially reduced, the recovery rate is improved, and the precise detection of tirofiban is achieved. The method is successfully applied to detection of tirofiban in liver tissues in a clinical animal experiment stage, has important clinical significance for monitoring the in-vivo content of tirofiban after medication, avoids occurrence of postoperative related complications in serious interventional therapy, and has a good clinical application prospect. And side effects such as poor thrombolysis effect caused by insufficient medication and bleeding caused by excessive medication are prevented.

The invention provides a lung-kidney interaction simulation system based on an organ chip, and the system comprises a lung chip module which is used for simulating a qi-blood barrier structure of a lung, and the lung chip module is inoculated with human alveolar epithelial cells and human lung microvascular endothelial cells; the kidney chip module is used for simulating a capillary lumen of a kidney tubule and a kidney tubule lumen structure, and human kidney tubule epithelial cells and human umbilical vein endothelial cells are inoculated on the kidney chip module; the connecting channel is used for communicating the lung chip module with the kidney chip module and allowing the culture medium to flow between the lung chip module and the kidney chip module; and wherein a klebsiella pneumoniae liquid for causing lung injury is applied to the upper layer of the lung chip module. The invention provides a novel simulation system for the research of lung-kidney interaction, and solves the limitation of animal experiments.

The invention provides a traditional Chinese medicine preparation for improving or treating cognitive impairment, and relates to the technical field of traditional Chinese medicine pharmacy, and the traditional Chinese medicine preparation is prepared from morinda officinalis, eclipta, semen boitae, fructus alpiniae oxyphyllae, bighead atractylodes rhizome, valerian, turmeric, rhizoma acori graminei, leech, rhizoma anemarrhenae, dendrobium officinale, endothelium corneum gigeriae galli, hematoxylon and honey-fried licorice root. The traditional Chinese medicine preparation shows remarkable neuroprotection and cognition improvement effects on the cell level and the animal level, in the cell level, the preparation can effectively resist Abeta1-42 induced cell damage, and the protection effect is achieved through multiple ways of improving the cell activity, enhancing the oxidation resistance, inhibiting neuroinflammation and the like; in animal experiments, the preparation can comprehensively improve cognitive impairment of APP / PS1 model mice, including relieving behavioral abnormality, improving spatial learning and memory ability, enhancing new object recognition ability, and effectively reducing deposition of A beta in hippocampus on a molecular level. The invention provides a new thought for improving or treating cognitive impairment.

This invention belongs to the field of biomedical technology, specifically relating to a madder-derived exosome-like nanovesicle, an artificial blood vessel, and their preparation and application. This invention utilizes differential centrifugation and sucrose density gradient centrifugation techniques to extract high-purity, highly active exosome-like nanovesicles from madder. Then, polydopamine is loaded onto the surface of a small-diameter polyurethane artificial blood vessel, which is then immersed in a suspension of madder-derived exosome-like nanovesicles to obtain a small-diameter artificial blood vessel loaded with madder-derived exosome-like nanovesicles. These madder-derived exosome-like nanovesicles can effectively promote endothelial cell proliferation and inhibit reactive oxygen species generation; animal experiments show unobstructed blood flow under ultrasound. The small-diameter artificial blood vessel loaded with madder-derived exosome-like nanovesicles can simultaneously achieve in vivo endothelialization and vascular patency, possessing significant clinical application value.

This disclosure provides siRNAs that target and regulate HBV gene expression and their applications. The double-stranded RNAi agent comprises an antisense strand and a sense strand complementary to the antisense strand forming the double-stranded region. The nucleotide sequence of the antisense strand is shown in SEQ ID NO:13, or the nucleotide sequence of the antisense strand is a modified sequence of the sequence shown in SEQ ID NO:13. Cellular and animal experimental results show that the double-stranded RNAi agent provided in this disclosure can significantly reduce the expression of one or more HBV genes, block the viral life cycle, and can be used to develop drugs for treating HBV gene expression-related diseases.

The invention relates to a preparation method and antibacterial application of an adjustable manganese-based Prussian blue analogue. Through metal ion doping and lattice engineering regulation and control, a manganese element is introduced into a Prussian blue frame structure, and controllable regulation of the doping amount and optimization of a crystal structure are realized. The prepared material has excellent photothermal conversion capability and multienzyme simulation activity, and shows a photothermal-enzyme synergistic enhancement effect under the irradiation of near-infrared light. In-vitro studies show that the material can efficiently inhibit the growth of gram-negative bacteria; animal experiments further verify that the composition has a remarkable curative effect in infected wound repair. The invention not only provides a preparation method of a nano material with controllable metal doping, but also provides a novel photo-enzyme synergistic treatment method for drug-resistant bacterium infection treatment.

The invention discloses an in-vitro drug intestinal permeability determination method based on a high-flux barrier chip, and belongs to the technical field of microfluidics and organ chips. The high-flux barrier chip adopts a three-layer structural design of a pore plate layer, a porous membrane layer and a channel layer; the porous membrane not only realizes physical separation of the pore plate layer and the channel layer, but also undertakes the core function of interlayer substance exchange, and by matching with a gravity flow driving system provided by the swing table, the fluid dynamic characteristics in the chip are guaranteed. The hole pitch of the chip is strictly matched with the standard of a commercialized pore plate, and the chip can be directly compatible with various conventional analytical instruments and has good universality. The method is suitable for determining the intestinal permeability of the passive transport compound which can be absorbed by intestinal cells. Compared with the prior art, the human intestinal cells are adopted to construct the chip model, animal experiments do not need to be carried out, related ethical disputes are thoroughly avoided, the whole operation process is simple and easy to implement, and the detection time is greatly shortened.

The present application relates to a new use of Fuling Gancao Decoction, and specifically discloses application of Fuling Gancao Decoction in treatment of hyperlipidemia, wherein the Fuling Gancao Decoction is composed of the following components in parts by weight: Fuling 2 parts, Guizhi 2 parts, Zhigancao 1 part and Shengjiang 3 parts. It is found through animal experiment research that the Fuling Gancao Decoction can improve the symptoms of hyperlipidemia rats, reduce the content of TG, TC and LDL-C in serum, improve the liver lipid, blood sugar of hyperlipidemia model animals, reduce the liver coefficient, improve the liver cell fatty degeneration, reverse the pathological morphology of adipose tissue cells and the mitochondrial damage of hyperlipidemia model animals. A new indication of the Fuling Gancao Decoction is found, the development of traditional Chinese medicines is promoted, and a new drug composition with less side effects is provided for treatment of hyperlipidemia.

The invention discloses a method for preparing a phosphorylated salmon collagen peptide-calcium chelate by taking salmon collagen peptide as a raw material, preparing high-calcium binding active peptide (P-CP) through phosphorylation modification and chelating the high-calcium binding active peptide with calcium ions. The method comprises the following steps: (1) enzymolysis preparation of salmon bone collagen; (2) phosphorylation modification of a peptide fragment mediated by sodium tripolyphosphate; and (3) optimizing a calcium ion chelation process. The chelating rate of the obtained P-CP-Ca calcium reaches 91.21%, and structural characterization proves that a phosphate group (PO4 < 2->) is combined with calcium ions through ionic bonds. Animal experiments show that P-CP-Ca can significantly improve the serum calcium level of a calcium-deficiency mouse (the H group recovers to a normal range), reduce the activity of a bone resorption marker TRAP (Target Related Amplified Protein) (which is 25.4% lower than that of a model group), and improve the bone trabecula microstructure (the bone mineral density is improved by 32.7%). The invention provides a new technology for valuable utilization of fishery by-products and development of functional calcium supplements.

This invention relates to a radiotherapy device and experimental method with switchable high and low dose distributions, belonging to the field of radiotherapy experimental technology. It solves the problem in existing technologies where the radiation dose in low-dose experiments in animal radiotherapy cannot be controlled. The invention includes a shielding platform and a shielding end; the shielding end is rotatably mounted on the shielding platform and has light-transmitting holes arranged in an alternating pattern. In this invention, the radiation beam emitted by the radiation source device directly irradiates the experimental animal, allowing for control of the radiation dose, and the high-dose and low-dose irradiation points on the experimental animal are arranged alternately.

The application discloses a traditional Chinese medicine composition with the effect of treating infantile recurrent respiratory tract infection and a preparation method thereof. Through a large amount of clinical practice, the application selects radix pseudostellariae, fried atractylodes, fried rhizoma atractylodis, agastache, levisticus, lonicera, radix scrophulariae, carbonized wheat bran, calcined osseous, ephedra root as raw materials, and is prepared into an oral or external dosage form according to weight ratio. The application has the effects of transporting the spleen, preventing the invasion of pathogenic factors, and consolidating the surface and stopping sweating, and is used for infantile recurrent respiratory tract infection with the syndrome of the spleen failing to transport and the lung and defensive system failing to consolidate. The application is verified by clinical tests, has remarkable curative effect, no toxic side effect, and good safety. Animal experiment researches show that the traditional Chinese medicine composition can effectively relieve the chronic inflammation level of model mice, improve the airway epithelial barrier damage, improve the body's ability to resist pathogenic factors, and reduce the risk of infection. The application can fill the market blank of treating infantile recurrent respiratory tract infection diseases based on the theory of 'four seasons with the spleen being strong and not being invaded by pathogenic factors' and 'the spleen being strong is not about tonifying but about transportation', and has great development prospect.

This invention discloses *Pediococcus lactis* TC-4, which simultaneously degrades nucleosides, purines, and uric acid, and its applications. *Pediococcus lactis* ( Pediococcus acidilactici This invention relates to *Pediococcus lactis* TC-4, with accession number GDMCC No: 67936. *Pediococcus lactis* TC-4 of this invention can efficiently degrade three nucleosides (guanosine, adenosine, and inosine), three purines (guanine, xanthine, and hypoxanthine), and uric acid in vitro. *Pediococcus lactis* TC-4 of this invention can effectively reduce serum uric acid levels in broiler chickens, alleviating and treating hyperuricemia. Compared with traditional chemical drugs for treating hyperuricemia and / or gout, this invention has no toxic side effects on the ecological environment, no residue risk, and is green and safe. This invention has been confirmed at multiple levels, including genetic and animal studies, to have no potential harm to humans, demonstrating its advantages of safety and high efficiency.

The invention belongs to the technical field of medicinal material screening, and relates to a traditional Chinese medicine compound for treating attention deficit hyperactivity disorder and a screening method thereof. The screening method comprises the following steps: constructing a drug use database of ADHD; performing statistics and association rule analysis on frequency, property and flavor tropism and efficacy of the traditional Chinese medicines to obtain a high-frequency traditional Chinese medicine composition; screening ADHD disease targets and establishing a target set; performing clustering analysis and integration on the high-frequency traditional Chinese medicine composition to obtain a core medicine composition; constructing candidate traditional Chinese medicine compounds through reverse network pharmacological analysis; evaluating the efficacy of the candidate traditional Chinese medicine compound through animal experiments, and screening out the optimal traditional Chinese medicine compound. According to the method, an O-R-S analysis mode is constructed through data mining and reverse network pharmacology, traditional Chinese medicine compound screening and evaluation processes of ADHD and candidate compounds with better comprehensive performance are optimized, and a reference scheme with data support and mechanism basis is provided for traditional Chinese medicine clinical treatment and new drug research and development of ADHD.

This invention belongs to the field of biomedical technology, specifically involving Trmt61a The application of genes in the preparation of drugs for treating pathological myocardial hypertrophy. This invention provides... Trmt61a The application of genes in the preparation of drugs for treating pathological myocardial hypertrophy, the aforementioned Trmt61a The nucleotide sequence of the gene is shown in SEQ ID NO.1. Animal experiments have shown that knocking down... Trmt61a Gene knockdown can significantly improve cardiac contractile function after aortic arch coarctation surgery, alleviate cardiomyocyte hypertrophy, reduce the degree of cardiac fibrosis, and effectively treat pathological myocardial hypertrophy. This invention is the first to demonstrate knockdown... Trmt61a Genes can effectively improve pathological myocardial hypertrophy, providing a new drug development path and target for the prevention and treatment of this disease, and have important medicinal value and clinical application prospects.

This invention relates to the technical field of multi-channel ELISA reaction plates for detecting feline triple vaccine antibodies. It discloses a multi-channel ELISA reaction plate for detecting feline triple vaccine antibodies, comprising a reaction plate body, a base plate, and an ELISA reaction plate disposed on top of the base plate. The ELISA reaction plate has an FPV reaction channel, an FCV reaction channel, and an FHV reaction channel disposed on top of the ELISA reaction plate, and an ELISA plate sealing cap is snapped onto the top of the ELISA reaction plate. This invention enables multi-channel parallel reactions in the detection of feline triple vaccine antibodies, saving reagents, improving experimental efficiency, and is suitable for animal experiments, vaccine evaluation, and clinical sample testing. It is also sealable, stackable, and easy to transport.

This invention discloses a STAT3-mutated cell product and its uses, belonging to the interdisciplinary field of genetic engineering and tumor immunotherapy. Through saturation mutation screening, this invention is the first to discover that STAT3 gain-of-function mutations promote CAR-T anti-tumor responses. Furthermore, through cell and animal experiments, it is demonstrated that activating STAT3 can alleviate CAR-T cell immune exhaustion and significantly enhance the in vivo and in vitro killing ability of CAR-T cells against tumor cells, thereby improving the efficacy of anti-tumor therapy. This invention provides a new therapeutic target and strategy for the field of tumor immunotherapy, possessing significant scientific and clinical application value.

The invention relates to a big data screening method of a traditional Chinese medicine compound for treating enteritis and application thereof, and belongs to the field of traditional Chinese medicine screening based on a data mining technology, animal experiment efficacy investigation and network pharmacology prediction. Comprising the following steps: performing data arrangement specification on a traditional Chinese medicine compound for treating enteritis based on a data mining technology; analyzing and summarizing the standardized data to obtain a new traditional Chinese medicine compound (AEND); constructing an acute enteritis mouse model, and performing pharmacodynamic evaluation on the traditional Chinese medicine compound; predicting the action mechanism of the screened traditional Chinese medicine compound based on a network pharmacology method; a molecular docking technology is applied to virtually verify the action target of the traditional Chinese medicine compound on acute enteritis; the pathway protein of a mammalian cell receptor tyrosine kinase mediated phosphatidylinositol related (PI3K / AKT) signal pathway is detected. According to the invention, a new formula AEND is screened out based on data mining, and a pharmacological experiment verifies that the AEND has a protective effect on mice with acute enteritis. The developed traditional Chinese medicine compound has the effects of resisting inflammation and regulating the homeostasis of intestinal flora.

The invention provides application of an expression inhibitor of KMT5A in preparation of a medicine for preventing or treating esophageal squamous carcinoma. In-vitro experiments prove that ICA can inhibit proliferation of esophageal squamous carcinoma cells in a concentration-dependent manner and remarkably promote cell apoptosis. The action mechanism of the ICA is closely related to the direct targeting KMT5A protein, and experiments show that the ICA can reduce the expression level of the KMT5A and influence the protein stability of the KMT5A. Clinical sample analysis further shows that KMT5A is highly expressed in esophageal cancer tissues, and ICA treatment in animal experiments significantly reduces the tumor volume. The invention provides a new targeted treatment strategy for esophageal squamous carcinoma, and has important clinical transformation value.

The application provides application of alnus ketone in preparation of a medicine for preventing and / or treating ulcerative colitis, and belongs to the technical field of traditional Chinese medicines. The application research finds that alnus ketone can significantly reduce secretion of intestinal epithelial cell inflammatory factor IL-8, and has an anti-inflammatory effect. Animal experiments show that alnus ketone can reduce weight loss of mice caused by ulcerative colitis, reduce severity of occult blood, and reduce shortening of the colon, indicating that alnus ketone has a protective effect on ulcerative colitis. The alnus ketone is applied to clinical treatment, and has the advantages of high efficiency, low toxicity, economy and feasibility.

The invention relates to the technical field of animal medicine, in particular to a self-adaptive control system and method for animal tracheal instillation. The system comprises a preoperative planning module which is used for reconstructing a three-dimensional model based on an individual animal airway CT image, simulating and optimizing administration parameters through computational fluid mechanics, and generating a personalized operation plan; the intraoperative navigation and execution module guides a catheter to reach a target pose through electromagnetic navigation, and performs closed-loop regulation and control on the instillation process through a respiration synchronous triggering algorithm and a pressure self-adaptive control algorithm based on real-time respiration and pressure signals; the central control module coordinates the work of each module; and the postoperative verification module quantifies actual and predicted deposition effects through image comparison. The problems that a traditional method depends on experience, cannot adapt to physiological changes in real time and is poor in drug administration precision are solved, the spanning from experience operation to individualized precise self-adaptive control is achieved, and the safety, repeatability and scientificity of animal experiments are remarkably improved.

The invention discloses application of a molecular target Ptchd1 in nerve synaptic function and consciousness regulation and related diseases. Whole genome screening finds that Ptchd1 is a key molecular target for consciousness regulation, and animal experiments prove that the target has an important regulation effect on neuronal synaptic vesicle release and consciousness disappearance caused by general anesthesia drugs. In view of the fact that Ptchd1 can regulate and control drug-induced awareness disappearance and neuronal synaptic delivery, related treatment with Ptchd1 as a target spot has significant potential values in the following fields: i) application in preparation of reagents for regulating and controlling a neuronal synaptic function and awareness regulation and control; the invention also relates to application of the pharmaceutical composition in preparation of drugs for promoting awakening after anesthesia and application of the pharmaceutical composition in preparation of drugs for promoting awakening of diseases related to disturbance of consciousness, wherein the diseases related to the disturbance of consciousness comprise vegetative people, micro-consciousness or brain injury coma.

The invention discloses a siRNA duplex for targeted regulation and control of INHBE gene expression and application of the siRNA duplex in prevention and treatment of metabolism-related diseases. The present disclosure also relates to corresponding conjugates and pharmaceutical compositions. Cell and animal experiment results show that the oligonucleotide duplex can obviously inhibit the expression of the INHBE gene, and can be used for preparing medicines for preventing or treating related diseases such as atherosclerosis.

The invention relates to the field of traditional Chinese medicines, in particular to novel application of a Sweeping Tianhu lung-clearing and cough-relieving composition in treatment of chronic obstructive pulmonary disease. Animal experiment studies show that the Sweetio lung-clearing and cough-relieving composition can significantly improve the lung function of COPD mice and inhibit bronchial abnormal remodeling. The action mechanism of the composition is related to inhibition of lung tissue cell apoptosis, alleviation of inflammatory cell infiltration and improvement of abnormal remodeling of bronchus, which indicates that the Sweeping Litu composition for clearing away lung-heat and relieving cough has obvious pharmacological activity of resisting COPD (chronic obstructive pulmonary disease).

The invention belongs to the technical field of nano materials and biological medicines, and relates to carrier-free self-assembled drug nanoparticles as well as a preparation method and application thereof. The carrier-free self-assembled drug nanoparticle provided by the invention is formed by self-assembling four components, namely a chemotherapeutic drug, a photosensitizer, a biomineralized shell and a cancer cell membrane; wherein the chemotherapeutic drug is doxorubicin hydrochloride, the photosensitizer is indocyanine green, the biomineralized shell is prepared from a potassium permanganate solution, and the cancer cell membrane is a 4T1 cancer cell membrane. The invention also provides a method for preparing the nanoparticles, a drug containing the nanoparticles and application of the nanoparticles in preparation of tumor treatment drugs. The nanoparticle provided by the invention has the characteristics of high drug loading rate, simple preparation and no carrier, and in-vitro cell experiments and animal experiments prove that the nanoparticle has a remarkable tumor treatment effect.

The invention belongs to the technical field of microorganisms, and particularly discloses a composition with anti-aging and anti-saccharification functions and application of the composition. The composition is prepared from the following components: lactobacillus paracasei BL-22 and stachyose, wherein the lactobacillus paracasei BL-22 is prepared from lactobacillus paracasei BL-22 and stachyose; the preservation number of the lactobacillus paracasei BL-22 is CGMCC (China General Microbiological Culture Collection Center) NO. 33475. Through animal experiments and research on interaction of the two substances, the invention reveals that the lactobacillus paracasei BL-22 and the stachyose have a synergistic effect mechanism for the first time, and confirms that the lactobacillus paracasei BL-22 and the stachyose are combined to generate a remarkable synergistic effect in the aspects of relieving aging and skin saccharification. The invention provides a safe and convenient new scheme for solving the problems of skin aging and saccharification, and also provides a new research direction and technical support for development of aging health related products.

The invention discloses application of steroid saponin of trillium tschonoskii maxim to preparation of a medicine for treating vascular dementia, and relates to the technical field of medicines. Animal experiments prove that the berberis apiacea steroid saponin can improve the spatial reference learning function of cerebral ischemia rats, improve the spatial reference memory function of the cerebral ischemia rats and enhance the long-term memory function of the cerebral ischemia rats. In addition, brain tissue damage and white matter structure damage of rats suffering from cerebral ischemia can be relieved, axonal myelin sheath microstructure reconstruction and white matter fiber reconstruction of the rats suffering from cerebral ischemia are promoted, and myelin sheath regeneration of rats suffering from vascular dementia is promoted. Network pharmacological analysis finds that the age grass total steroid saponin has a wide regulation effect on OPCs and OLs cell components and molecular functions, and further, OPCs differentiation myelin sheath inhibition related factors are regulated and controlled, and white matter myelin sheath regeneration is promoted.