319 results about "Animal study" patented technology

The invention belongs to the field of biological pharmacy, and relates to a temperature-sensitive hydrogel, in particular to a temperature-sensitive hydrogel SV (at) HP based on hyaluronic acid as well as a preparation method and application of the temperature-sensitive hydrogel SV (at) HP. The temperature-sensitive hydrogel SV (at) HP entraps CS-siRNA nanoparticles and VEGF (at) PLGA microspheres, and is used for realizing microenvironment responsive drug delivery to treat intrauterine adhesion. Chlorogenic acid and TGF beta1 siRNA are self-assembled through disulfide bonds to form CS-siRNA nano-particles, the CS-siRNA nano-particles are used for treating intrauterine adhesion through gene and chemical therapy, and cell and animal experiments prove that the CS-siRNA nano-particles have a good effect of improving intrauterine adhesion. Through evaluation of a real-time fluorescence quantification method, a western blot method, a cell scratch experiment, laser confocal microscope observation, inverted fluorescence microscope observation, an in-vivo experiment and the like, the SV-coated HP gel has the effects of resisting inflammation, improving endometrial fibrosis, promoting IUA rat endometrial angiogenesis and repair, inhibiting intrauterine adhesion and improving the intrauterine environment.

The invention provides a target Her2 nuclide probe as well as a preparation method and application thereof, and relates to the technical field of nuclide probes. The target Her2 nuclide probe comprises a compound which is labeled by radionuclide and is shown in a formula I. The target Her2 nuclide probe can be used as a diagnosis and treatment reagent in human or animal Her2 protein high expression lesions, and is especially used as a tumor imaging agent and a radionuclide treatment drug. In animal experiments, the tumor / muscle ratio of the radioactive complex is high, and the radioactive complex has a good application prospect. And the formula I of the # imgabs0 # is shown in the specification.

The invention discloses a micro-fluidic chip system for simulating a cartilage mechanical loading environment, and belongs to the technical field of biological micro-fluidic chips. A micro-fluidic chip and a peripheral system are designed according to a biomechanical principle and a micro-fluidic chip technology, and a real cartilage microstructure and a mechanical environment can be simulated. The micro-fluidic chip simulates a real cartilage structure and is used for providing internal synovial fluid pressure. The peripheral system provides a controllable external mechanical environment, internal synovial fluid pressure and external three-dimensional mechanical loading of the chip are controlled through PID feedback, a platform is provided for researching a synergistic effect mechanism of the internal synovial fluid pressure and the external three-dimensional mechanical loading, and the peripheral system can also observe diffusion movement of internal nano-particles in the mechanical environment in real time. The system provides an experimental platform for researching the influence and mechanism of mechanical stimulation on OA cartilage cell functions, avoids the defects of ethical problems and individual differences in previous animal experiments, is simpler and more convenient to operate, lower in cost and shorter in experimental period, and provides a scientific basis for researching the influence of mechanical loading on cartilage repair.

The invention relates to a human recombinant GDF15 protein and application thereof in preparation of drugs for preventing and treating sepsis acute kidney injury, and belongs to the technical field of biological medicines. In order to solve the problem that an effective prevention and treatment scheme for improving SA-AKI by inhibiting oxygen death is lacked in existing clinical and research, the invention provides a human recombinant GDF15 protein of which the coding gene nucleotide sequence is shown as SEQ ID No: 1. Animal experiment results show that by intraperitoneal injection of the human recombinant GDF15 protein, kidney inflammation of sepsis mice can be relieved, and expression of PGAM5 can be down-regulated. Cell experiment results show that when the human recombinant GDF15 protein is given, LPS-induced USF2 increase can be inhibited. The human recombinant GDF15 protein provided by the invention can improve SA-AKI by inhibiting oxygen death and inflammation, provides a new idea and an intervention target for prevention and treatment of SA-AKI, and shows a good clinical application prospect.

The invention relates to the technical field of data management and information systems, in particular to an experimental data management system conforming to GLP specifications, which comprises a data acquisition and standardization unit, a data storage and version management unit, a natural language query and interaction unit, a data quality and compliance verification unit and an audit tracking and authority management unit. According to the method, professional terms and query intentions are accurately analyzed by means of a field-specific language model, intelligent recognition and visual interaction of image data are realized by the multi-modal interaction module, dialogue continuity is maintained by the context dialogue management module, and an association analysis scheme is actively pushed by the intelligent recommendation module. Intelligent retrieval and analysis requirements of animal experiment data are efficiently supported, the compliance and quality of the experiment data are guaranteed, and data risks are effectively identified and handled.

A pharmaceutically acceptable salt of a polypeptide and a use thereof. According to the pharmaceutically acceptable salt of the polypeptide, the half-life of a drug is prolonged by means of a study on the structure-activity relationship and structural modification, the in-vivo exposure is increased, the number of times of administration is reduced, the side effect in animal experiments is lower, and the therapeutic window of the drug is larger. The pharmaceutically acceptable salt of the polypeptide can be used for treatment of ETBR receptor-related diseases, such as rehabilitation treatment of the acute phase and the recovery phase of a cerebral arterial thrombosis patient, promoting better recovery of the stroke patient.

The present disclosure provides a modified oligonucleotide sequence and its application. A series of siRNAs are designed based on the angiotensinogen (AGT) messenger ribonucleic acid (mRNA) sequence, and are alternately modified and modified using a specific set of modification templates. The results of cell and animal experiments show that some alternately modified and specifically template-modified oligonucleotide sequences can significantly inhibit the expression of the AGT gene, and can be used for developing drugs for treating related diseases such as hypertension.

The utility model discloses a pair of adjustable nerve bruise forceps with pressure sensing function, which comprises a first forceps body, a second forceps body and a bruise clip, one end of the forceps body far away from a user is provided with a buckle, the buckle is connected with the bruise clip, and the first forceps body and the second forceps body drive the bruise clip to close after applying external force so as to bruise nerves. The bruise clamp is provided with various types of adaptive buckles, the bruise width of the nerve bruise clamp is adjusted to adapt to different experiment requirements and requirements of experiment objects, a pressure sensor is arranged at the position, close to the buckles, of the clamp body, the pressure sensor sends a sensing signal to a controller, an operator can control own force through a display screen of the sensor, and the operation is convenient. And the nerve injury degree is kept consistent. The dependence of the crushing degree on an operator is reduced, the operation fatigue is relieved, and a more accurate, scientific and efficient medical instrument is provided for animal experiment modeling of nerve crushing injuries.

The invention discloses application of 9 ''-salvianolic acid B monomethyl ester in preparation of a medicine for treating ischemic heart disease, and belongs to the technical field of biological medicine. The CAS number of the 9 ''-salvianolic acid B monomethyl ester is 1167424-31-8, the ischemic heart disease refers to myocardial injury induced by heart ischemia reperfusion, and the invention further provides a pharmaceutical composition or a small molecule injection preparation. Animal experiments show that 9 ''-salvianolic acid B monomethyl ester is exogenously injected to relieve myocardial injury of a mouse and improve the heart function of the mouse, and it is proved that the medicine containing 9''-salvianolic acid B monomethyl ester can improve the ischemic heart disease, especially myocardial injury caused by heart ischemia reperfusion, and has a good application prospect. The invention provides potential drug selection and research direction for preventing and treating ischemic heart disease, and has important clinical application value.

The invention provides a radix tetrastigme polysaccharide and application thereof in a medicine for treating diabetic retinopathy. The radix tetrastigme polysaccharide is prepared by the following steps: deproteinizing a water extract of radix tetrastigme by using CaCl2, depigmenting by using activated carbon, performing column chromatography, eluting by using a NaCl solution, merging and collecting eluent components, concentrating under reduced pressure, dialyzing by using a dialysis bag with an interception value of 0.5 KDa, and performing vacuum freeze drying. And performing further chromatography by using Chromdex 200PG sephadex gel, collecting the components, and performing freeze drying to obtain the homogeneous polysaccharide with the average molecular weight of 36.848 KDa. Meanwhile, pharmacological activity evaluation is carried out on the radix tetrastigme polysaccharide, it is found that the polysaccharide can reduce the free Fe < 2 + > level and reverse high glucose induced ARPE19 cell damage, and in addition, animal experiments also prove that the radix tetrastigme polysaccharide can reduce the DR rat retina Fe < 2 + > level and relieve diabetic retinopathy; the research can provide a scientific basis for research and development of a novel natural medicine radix tetrastigme polysaccharide for treating DR.

The invention discloses a key effect and a targeted intervention strategy of YTHDF1 in regulation and control of a hepatocellular carcinoma immunosuppression microenvironment. In the tumor-associated fibroblasts, YTHDF1 enhances the translation efficiency of OTUD5 mRNA through an m6A dependency mechanism, an up-regulated deubiquitination enzyme OTUD5 is directly combined with a tryptophan metabolic rate-limiting enzyme IDO1, ubiquitination degradation of the tryptophan metabolic rate-limiting enzyme IDO1 is inhibited, tryptophan is further driven to catabolism to generate kynurenine, regulatory T cell differentiation is promoted, and an immunosuppression microenvironment is formed. Spatial transcriptomics shows that YTHDF1 + CAFs and Tregs are significantly co-localized in tumor tissues and have metabolic interaction. Based on artificial intelligence virtual screening, it is found that a natural compound hesperidin can be specifically combined with a YTH structural domain of YTHDF1, an OTUD5-IDO1 signal axis mediated by the natural compound hesperidin is blocked, and the KYN level is remarkably reduced. Animal experiments prove that the tumor inhibition rate of combined treatment of hesperidin and anti-PD-1 is increased by 40% or above compared with that of a single drug group, and synergistic interaction is achieved by inhibiting Tregs infiltration and enhancing the activity of cytotoxic T lymphocytes.

The invention requests to protect an automatic laboratory mouse wound area tracking method and system based on image recognition, images of a wound area are regularly acquired through a multispectral imaging device, parameters such as ambient light and shooting distance are synchronously recorded in the acquisition process to ensure data consistency, the images are preprocessed by using a distributed computing system, and the data consistency is ensured. Inputting the preprocessed image into a deep segmentation model trained based on a large-scale data set to accurately identify and output a binarized contour mask of a wound region, calculating wound geometric features based on the mask, and dynamically generating a quantitative report including an area change rate and a healing trend coefficient; all time sequence data are stored in a time sequence database and used for constructing a complete wound healing track map, and visualization and trend analysis of the healing process are supported. According to the invention, high-flux, automatic and quantitative monitoring of the wound healing process of the laboratory mouse is realized, and objectivity and analysis efficiency of animal experiment data are remarkably improved.

The invention discloses a microorganism-intestine-joint axis multi-organ chip and a preparation method thereof, and belongs to the field of biomedical engineering.The preparation method comprises the following specific steps that firstly, an upper-layer chip male mold, a middle-layer chip male mold and a lower-layer chip male mold of the multi-organ chip are prepared through the high-precision 3D printing technology; iI, processing the silicon wafer with the cylindrical microarray through photoetching to prepare a porous membrane; iII, preparing an upper-layer chip, a middle-layer chip and a lower-layer chip; iV, assembling each group of prepared chips; according to the invention, human cells or organoids can be used for experiments, the experiment result has good correlation with human beings, and there is no species difference between animal experiments and human beings; the experiment process can be obviously accelerated, and the experiment time is shortened; according to the method, the experimental cost can be greatly saved, and the dispute of experimental ethics does not exist, so that the method can be expanded to large-scale standardized processing production so as to carry out high-throughput and large-scale experimental research.

The invention relates to a radionuclide labeled trimer probe 68Ga-TRAP-(FAPI) 3 targeting FAP and a preparation method thereof, Trap is used as a trivalent chelating platform, and three FAPI targeting units are covalently connected by clicking a chemical linking arm to form a trimer structure; the invention further relates to application of the 68Ga-TRAP-(FAPI) 3 probe in preparation of imaging agents or therapeutic drugs for diagnosing FAP positive diseases, experiments prove that the trimer probe is good in affinity and high in stability, and in cell experiments and animal experiments, the uptake rate of FAP positive cells is kept at a high level within 240 minutes; preclinical and preliminary clinical applications show that the tumor detection rate of the < 68 > Ga-TRAP-FAPI 3 in various cancer models is superior to that of < 18 > F-FDG, and compared with < 68 > Ga-FAPI-04, the < 68 > Ga-TRAP-FAPI 3 is clearer to display focuses (particularly tiny metastases), higher in uptake intensity and particularly outstanding in delayed imaging, and the < 68 > Ga-TRAP-FAPI 3 can be used for preparing a medicine for treating cancer. And a novel drug candidate with better performance is provided for precise diagnosis and staging of tumors and subsequent radionuclide targeted therapy.

The invention relates to the field of medicines, in particular to application of an ADRB2 agonist in preparation of a medicine for preventing and / or treating sepsis. Cell experiments and animal experiments find that expression of IL-10 in a sepsis model mouse body can be hindered and the death rate can be increased by knocking out Adrb2 in B cells, and the death rate of the sepsis model mouse can be reduced by administering an ADRB2 activating agent. The ADRB2 agonist can be used for preventing and / or treating sepsis, and a new thought is provided for clinical medication of sepsis.

The application discloses a fish scale protein polypeptide anti-chronic heart failure efficacy evaluation system, relates to the technical field of biological active polypeptide evaluation and cardiovascular disease function analysis, and comprises a raw material processing module for preparing fish scale protein polypeptides, a CHF model construction module for animal experiments, a function detection module, and a data processing and analysis module. Multidimensional statistics and visual algorithm are adopted to integrate tissue remodeling indexes such as heart function, oxidative stress and inflammatory reaction, so that comprehensive evaluation of the efficacy of fish scale protein polypeptides is realized. The application can realize systematic, quantitative and mechanistic evaluation of the anti-chronic heart failure effect of fish scale protein polypeptides, solve the problems of single evaluation index, poor repeatability of results and lack of mechanism correlation in the prior art, and has the advantages of comprehensive evaluation, reliable results, wide application range and strong generalizability.

The application relates to the technical field of cell in-vitro culture, and discloses a sphincter in-vitro culture environment construction device which comprises a culture device and a control host computer, the culture device is internally provided with a contraction member, the control host computer is connected with the culture device in a matched mode, and is used for regulating and controlling the action of the contraction member, so as to construct a physiological contraction environment. The application can simulate the annular contraction and the physiological contraction characteristics of the high and low pressure of the human sphincter, restore the dynamic contraction process, adopt a detachable structure, adapt to different culture cavities, has strong experimental flexibility, combines a perfusion design with a three-dimensional culture layer, simulates in-vivo nutrition transportation and metabolism discharge, provides a bionic growth environment for cells, integrates strain force detection and temperature control closed-loop regulation, is accurate in parameters and reliable in data, makes up for the short board of traditional static culture and animal experiments, provides a stable and efficient experimental platform for sphincter disease mechanism, mechanical response research and drug screening.

The application discloses application of a reagent for detecting LRRN3 expression level in preparation of a benign prostatic hyperplasia diagnosis product. It is proved through cell function experiments and animal experiments that overexpression of the LRRN3 gene can inhibit the proliferation of prostate cells, block the cell cycle and promote cell apoptosis, thereby effectively inhibiting the prostatic hyperplasia. The discovery provides a new target and treatment strategy for the treatment of the prostatic hyperplasia, and provides a brand-new thought and method for the treatment of the prostatic hyperplasia. Based on the research results, a gene therapy drug taking the LRRN3 gene as a target can be further developed, for example, a lentivirus vector carrying the LRRN3 gene or other gene delivery systems are constructed and used for the treatment of the prostatic hyperplasia. In addition, the LRRN3 gene can be combined with other treatment methods to improve the treatment effect.

The invention discloses armillariella mycelium polysaccharide ATMP capable of improving cognitive impairment and a preparation method and application of the armillariella mycelium polysaccharide ATMP. The polysaccharide is composed of two components, and the molecular weights of the two components are 2.30 * 10 < 4 > Da and 4.56 * 10 < 5 > Da respectively; the monosaccharide composition comprises mannose (Man), glucose (Glc) and galactose (Gal), and the molar ratio of the mannose (Man) to the glucose (Glc) to the galactose (Gal) is 1: 16.64: 1.96. Animal experiment results show that the armillariella mycelium polysaccharide ATMP can improve cognitive impairment induced by lipopolysaccharide (LPS) by improving the spatial memory ability of mice, repairing the disorder state and deletion injury of neurons in a hippocampal region and relieving neuroinflammatory response, so that the obvious neuroprotective effect is achieved. As a natural brain-benefiting active component, the armillariella tabescens mycelium polysaccharide ATMP has a wide application prospect in the related fields of brain health.

The present invention relates to the technical field of biology, and particularly relates to a method for inhibiting an inflammatory response by using a fibroblast. In the present invention, a fibroblast, which is obtained by digesting normal foreskin tissue by means of clostridiopeptidase A to isolate a cell as a seed cell and culturing the seed cell in vitro by using a serum-free medium, shows positive expression of CD105, CD90, CD73, COL1A1, and FAP, with expression rates all exceeding 80%, and negative expression of CD36, CD39, CD45, and HLA class II molecules, with expression rates below 5%. The fibroblast can regulate an immune response by secreting TGFβ, inhibiting proliferation of PBMCs, thereby inhibiting the inflammatory response, and has been verified in an animal experiment to have the ability to repair knee osteoarthritis caused by meniscus injury. Therefore, the fibroblast can be used for preventing and treating inflammatory lesions such as osteoarthritis.

The invention relates to the technical field of microecological preparations, in particular to a composition and application thereof in preparation of a product for improving liver injury. The novel composition provided by the invention contains probiotics, a radix puerariae extract and a grape skin extract, and the composition can effectively improve liver injury from an intestine-liver axis mechanism level. Animal tests prove that the effect is very ideal, the main components have a synergistic effect, the effect of remarkably improving liver injury can be achieved from multiple mechanism levels, and the traditional Chinese medicine composition is safe and free of side effects.

The application discloses a method for establishing a mouse model of MHC haplotype compatible allogeneic hematopoietic cell transplantation, and belongs to the technical field of animal experiment model construction. The technical problem to be solved by the application is how to construct a mouse model which can be used to study the immune reconstruction mechanism and treatment effect evaluation of MHC haplotype compatibility and MHC full compatibility in bone marrow cell transplantation recipient mice. To solve the technical problem, the application provides a method for constructing a mouse model of MHC haplotype compatible allogeneic hematopoietic cell transplantation, which comprises transplanting bone marrow cells of a donor mouse into a recipient mouse, and the MHC haplotype of the donor mouse and the recipient mouse is compatible. The application sets a modeling condition, and the model establishment condition is evaluated by survival condition, graft-versus-host disease and rejection reaction observation, flow cytometry and histopathological section, so as to prove that the model is reliable and stable.

The invention provides a liquid phase tandem time-of-flight mass spectrometry detection method for the content of tirofiban in liver tissue, and belongs to the technical field of detection. According to the present invention, the concentration of tirofiban in the liver tissue is detected through the liquid phase tandem time-of-flight mass spectrometry, such that the characteristics of low detection limit, immediate analysis, high sensitivity, good repeatability, good indoor reproducibility and high accuracy are provided, the matrix effect is substantially reduced, the recovery rate is improved, and the precise detection of tirofiban is achieved. The method is successfully applied to detection of tirofiban in liver tissues in a clinical animal experiment stage, has important clinical significance for monitoring the in-vivo content of tirofiban after medication, avoids occurrence of postoperative related complications in serious interventional therapy, and has a good clinical application prospect. And side effects such as poor thrombolysis effect caused by insufficient medication and bleeding caused by excessive medication are prevented.

The present invention discloses a detection kit and a detection method for type B botulinum neurotoxin. The present invention can complete qualitative detection of various suspected cases of botulinum neurotoxin poisoning in a short time, provide evidence support for assisting doctors in diagnosis and timely treatment decisions, and is of great significance in social health and safety. At the same time, the detection method of the present invention can significantly improve the specificity of toxin proteins, avoid the time problems caused by animal experiments such as serotype identification, and can be applied to the research and development and production process of botulinum neurotoxin products. In addition, the present invention can perform quality control on the stability and shelf life of botulinum neurotoxin standards.

This invention discloses the isolation, extraction, and novel application of a neutral, water-soluble polysaccharide from Sophora japonica extract. The invention extracts a water-soluble, neutral polysaccharide with a molecular weight between 20-22 kDa. This invention uses the Sophora japonica extract to treat renal interstitial fibrosis. Multiple studies, including cellular and animal studies, have demonstrated that the traditional Chinese medicine Sophora japonica can inhibit renal interstitial fibrosis, making it a potential drug for clinical application and bringing greater benefits to patients with kidney disease.

The invention provides a lung-kidney interaction simulation system based on an organ chip, and the system comprises a lung chip module which is used for simulating a qi-blood barrier structure of a lung, and the lung chip module is inoculated with human alveolar epithelial cells and human lung microvascular endothelial cells; the kidney chip module is used for simulating a capillary lumen of a kidney tubule and a kidney tubule lumen structure, and human kidney tubule epithelial cells and human umbilical vein endothelial cells are inoculated on the kidney chip module; the connecting channel is used for communicating the lung chip module with the kidney chip module and allowing the culture medium to flow between the lung chip module and the kidney chip module; and wherein a klebsiella pneumoniae liquid for causing lung injury is applied to the upper layer of the lung chip module. The invention provides a novel simulation system for the research of lung-kidney interaction, and solves the limitation of animal experiments.

The invention provides a traditional Chinese medicine preparation for improving or treating cognitive impairment, and relates to the technical field of traditional Chinese medicine pharmacy, and the traditional Chinese medicine preparation is prepared from morinda officinalis, eclipta, semen boitae, fructus alpiniae oxyphyllae, bighead atractylodes rhizome, valerian, turmeric, rhizoma acori graminei, leech, rhizoma anemarrhenae, dendrobium officinale, endothelium corneum gigeriae galli, hematoxylon and honey-fried licorice root. The traditional Chinese medicine preparation shows remarkable neuroprotection and cognition improvement effects on the cell level and the animal level, in the cell level, the preparation can effectively resist Abeta1-42 induced cell damage, and the protection effect is achieved through multiple ways of improving the cell activity, enhancing the oxidation resistance, inhibiting neuroinflammation and the like; in animal experiments, the preparation can comprehensively improve cognitive impairment of APP / PS1 model mice, including relieving behavioral abnormality, improving spatial learning and memory ability, enhancing new object recognition ability, and effectively reducing deposition of A beta in hippocampus on a molecular level. The invention provides a new thought for improving or treating cognitive impairment.

The invention discloses an extraction process method of a functional gastrodia elata wine partner and application of the functional gastrodia elata wine partner in alcoholic liver injury, and belongs to the technical field of biology. In order to improve the diversity of edible gastrodia elata products and solve the problems of long consumed time, low solubility, low leaching rate of active substances and the like of traditional gastrodia elata wine, the invention provides a new concept of gastrodia elata wine partners. Artificial intelligence and a response surface are combined, and optimal extraction process parameters are optimized and obtained as follows: the volume fraction of ethanol is 50%, the material-liquid ratio is 1: 20 g / mL, the ultrasonic time is 1.55 h, the ultrasonic temperature is 58 DEG C, and the ultrasonic power is 490 W; network pharmacology and molecular docking reveal that the gastrodia elata wine partner has a potential protection effect on alcoholic liver diseases; cell tests show that the gastrodia elata wine partner has a remarkable protection effect on HegG2 cells, and animal experiments further reveal that the gastrodia elata wine partner can effectively reduce the ethanol content of rat eye socket blood and has potential advantages in the aspects of reducing free radical damage and improving the oxidative stress state. The convenient edible product and the matching mode are expected to become beneficial supplement of the modern drinking culture, and the liver health is protected while people enjoy delicious wine.

The invention belongs to the technical field of biotechnology, and particularly relates to preparation and application of a CAR-NK cell targeting BCMA. According to the invention, an antibody sequence with patent medicine potential is obtained through hybridoma screening, and the sequence has originality. After humanized modification, the antibody still has relatively strong affinity to the antigen. Through CAR-NK preparation, it is found that the cells have a stronger tumor killing effect and have a very good anti-tumor effect in an MM mouse model body. The sequence of the BCMA antibody obtained through screening and the prepared CAR-NK cell targeting the BCMA show a good killing effect in in-vitro cell lines and animal experiments, and have the treatment potential for hematoma such as multiple myeloma.

An antisense oligonucleotide and the use thereof. A series of ASOs are designed on the basis of the genome sequence of a hepatitis B virus (HBV), and are modified by means of using a specific modification mode. Cell and animal experiment results show that some modified ASOs obtained by means of using the specific modification mode can significantly reduce the expression of one or more HBV genes and block the life cycle of viruses, and therefore can be used to develop drugs for treating HBV infection-related diseases.