Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method and application of deapio-platycodin D

A technology of platycodon saponin and desalpinose, which is applied in the field of preparation of cylindrocystis platycodon saponin D, can solve the problems of inability to fully utilize saponin resources, limited transformation paths of a single strain, etc., and achieves the effects of improving yield and environment-friendly production.

Pending Publication Date: 2020-07-10
YANBIAN UNIV
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, domestic and foreign studies on the biotransformation of saponins have found that the transformation pathway of a single strain is limited, and saponin resources cannot be fully utilized. Therefore, many scholars have begun to use mixed enzyme systems and mixed strains for biotransformation research, and the effect is ideal. Transformation from traditional single strain

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of deapio-platycodin D
  • Preparation method and application of deapio-platycodin D
  • Preparation method and application of deapio-platycodin D

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029] In order to make those skilled in the art more clearly understand the purpose, technical solutions and advantages of the present invention, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0030] The Pichia pastoris strain of the present invention is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, the preservation date is June 17, 2019, and the preservation number is CGMCC No.17939.

[0031] The present invention adopts the above-mentioned Pichia pastoris strain to prepare the platycodon saponin D method, comprises the following steps: culture step: the cultivation of said Pichia azwiya selects YPD liquid medium, yeast extract powder peptone glucose agar Culture medium (YPD); se...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
densityaaaaaaaaaa
melting pointaaaaaaaaaa
Login to View More

Abstract

The invention belongs to a preparation method of deapio-platycodin D. The preparation method for preparing the deapio-platycodin D is characterized in that the deapio-platycodin D is prepared by two or more bacterial strains, including a pichia kudriavzevii bacterial strain and a bacillus velezensis bacterial strain. According to the preparation method, two bacterial strains of different transformation mechanisms are used for the first time, and platycodin is transformed in a mixed fermentation manner, so that the yield of the deapio-platycodin D is effectively increased. Biotransformation ofthe platycodin is performed through the fermentation characteristics of the bacterial strains, so that safe and environment-friendly production of the platycodin can be realized, platycodon resourcescan also be sufficiently used, and the platycodin being low in bioactivity contained in the platycodon resources is transformed into saponin being high in bioactivity, and the effect of turning wasteinto wealth is achieved.

Description

technical field [0001] The present invention relates to a preparation method and application of de-apitoside platycodon saponin D, and relates to a preparation method of de-apitoside platycodon saponin D by adopting a mixed strain containing Pichia kudriazwii strain and Bacillus velesii. its application. Background technique [0002] Platycosides have the functions of treating hyperlipidemia, hypertension, diabetes, obesity, etc., and the main biologically active components are triterpene saponins (platycosides). According to the survey, more than 30 kinds of Platycodin saponins and various saponins have been isolated and identified. Platycodin D (Platycodin D), referred to as PD, is a white crystalline powder with a density of 1.56g / cm 3 , with a melting point of 228-237°C and a molecular formula of C 57 h 92 o 28 , relative molecular weight is 1224.58, good water solubility. Platycodon saponin D is a kind of platycodon acid disaccharide chain saponin, and its sugar gr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P39/00C12P33/20A61K31/704A61P1/16A61P3/04A61P3/10A61P13/12A61P19/02A61P29/00A61P35/00A61P37/02C12R1/84C12R1/07
CPCC12N1/16C12P39/00C12P33/20A61K31/704A61P35/00A61P19/02A61P3/04A61P13/12A61P1/16A61P3/10A61P29/00A61P37/02C12R2001/84C12N1/165Y02E50/10
Inventor 金清黄存辉朴泓洁李官浩崔福顺崔虎山
Owner YANBIAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com