23 results about "Serum urea" patented technology

The invention provides a combined lactobacillus salivarius YS-UR90 and a preparation method of the combined lactobacillus salivarius YS-UR90. The saliva combined lactobacillus provided by the invention can secrete protease and glycosidase, and the HCT and FLS gene expression levels of the saliva combined lactobacillus are high, so that the saliva combined lactobacillus can promote phenolic acid active ingredients to generate biotransformation during fermentation, and the proportion of chlorogenic acid and the like in fermentation liquor is increased. For example, marine fish oligopeptide and casein phosphopeptides are used as a composite nitrogen source, dandelion powder is used as a functional substrate, and a composite fermentation product obtained through fermentation of saliva and lactobacillus has the effect of promoting uric acid excretion and has a certain protection effect on kidney injury. The compound fermentation product has the effect of improving hyperuricemia mainly by inhibiting XOD activity, increasing serum urea nitrogen level, improving defect expression of uric acid synthesis gene HPRT1, inhibiting expression of uric acid reabsorption URAT1 and GLUT9 genes and improving expression of excretion genes OAT1 and ABCG2.

The application provides a kind of composition for relieving fatigue and its preparation method, belong to relieving fatigue composition technical field.The composition for relieving fatigue of the application includes the following raw materials by mass concentration: Brazilian ginseng-guarana composite water extract 60%-70%, taurine 1%-2%, tea polyphenol 0.2%-0.4%, inositol 0.8%-1.2%, maltitol 1%-1.5%, erythritol 0.5%-1%, stevioside 0.05%-0.1%, citric acid 0.01%-0.02%, malic acid 0.01%-0.02%, vitamin C 0.1%-0.3%, xanthan gum 0.08%-0.12%, and the balance is water.In the composition of the application, Brazilian ginseng-guarana composite water extract provides ecdysterone, caffeine and other core substances, laying the foundation for anti-fatigue;Taurine cooperates to remove lactic acid and reduce serum urea accumulation, inositol regulates energy homeostasis, tea polyphenol and vitamin C strengthen antioxidant protection, and each component plays a synergistic anti-fatigue effect through complementation, and the active ingredients are stable, low-calorie, and excellent in taste.

This invention discloses the application of quinolinic acid in the preparation of drugs for the prevention and treatment of acute kidney injury (AKI). AKI refers to acute kidney injury induced by renal ischemia-reperfusion. The drug is an oral formulation used for prophylactic administration in high-risk individuals undergoing iatrogenic procedures at potential AKI risk, and for pretreatment of ischemic conditions in transplanted kidneys. Experiments have shown that administration of quinolinic acid significantly reduces serum urea nitrogen levels in AKI mice, alleviates renal tissue morphological damage in AKI mice, and reduces the expression level of the renal tubular injury factor NGAL protein in AKI mice, demonstrating significant clinical value in the prevention and treatment of AKI.

The present invention discloses a method for constructing a mouse tail distal ischemia preconditioning renal protection model and its application, which belongs to the field of ischemia preconditioning technology, and its purpose is to solve the technical problem of poor stability and reliability of renal protection experimental results in the prior art. It is by respectively constructing a tail distal ischemia preconditioning group, a septic acute kidney injury model group and a tail control group; when constructing the tail distal ischemia preconditioning group, a tourniquet is tied on the mouse tail, and blood flow is restored by blocking blood flow and repeating the cycle; and blood and kidney tissue of mice in each group are collected for testing, serum creatinine and serum urea nitrogen indicators are measured, renal tubular pathological damage is assessed, and the expression level of renal reactive oxygen species is assessed. This method proves that tail ischemia reperfusion preconditioning can improve renal pathological damage and reduce renal tubular damage in septic acute kidney injury, inhibit renal oxidative stress in septic acute kidney injury, and reduce the inflammatory response of septic acute kidney injury.

The invention belongs to the technical field of biological medicines, and particularly discloses application of CDN1163 in preparation of a medicine for preventing or / and treating acute kidney injury. It is found for the first time that CDN1163 can relieve ischemia reperfusion induced AKI and reduce serum urea nitrogen and creatinine levels so as to play a role in protecting the kidney in the ischemia reperfusion induced AKI, and therefore the CDN1163 can be used for preparing the medicine for preventing or / and treating the acute kidney injury.

The invention relates to the technical field of preparation of probiotic fermented beverages, in particular to a probiotic fermented fruit and vegetable beverage with functions of resisting fatigue and enhancing immunity. The hawthorn fruit beverage specifically comprises the following components in parts by mass: 60-80 parts of Zhongtian big hawthorn fruits, 10-20 parts of autumn pears, 11-15 parts of grapes, 9-15 parts of kiwi fruits, 6-10 parts of pomegranates, 3-5 parts of figs, 3-5 parts of beet roots, 3-5 parts of pumpkins, 1-2 parts of kale, 1-2 parts of honey and 2-3 parts of zymophyte. The beverage product provided by the invention can significantly prolong exhaustive swimming time, improve liver glycogen reserve, reduce serum urea nitrogen level after exercises, and effectively relieve physical fatigue; meanwhile, the food can comprehensively enhance the immune function of the organism, including improving the activity of phagocytes, promoting the proliferation of lymphocytes and enhancing humoral immune response, and provides a natural and healthy solution for resisting fatigue and enhancing the immunity function through daily diet for consumers.

The application discloses alfalfa type non-antibiotic low-protein daily ration for reducing pig nitrogen emission and a preparation method thereof, which is composed of the following components in terms of dry matter weight fractions: corn 48-59 parts, soybean meal 1-5 parts, bran 1-2 parts, alfalfa powder 15 parts, wheat 16-23 parts, soybean oil 4-5 parts, calcium hydrogen phosphate 0.7-0.9 parts, stone powder 0-0.03 parts, salt 0.3 parts, lysine 0.3-0.5 parts, methionine 0.06-0.07 parts, threonine 0.14-0.21 parts, tryptophan 0.02-0.06 parts, valine 0.05-0.2 parts, isoleucine 0.04-0.2 parts, premix 1 part and compound probiotics 1 part. The daily ration improves the growth performance and meat quality of fattening pigs, improves feed conversion rate and economic benefits, reduces serum urea nitrogen content, promotes nitrogen metabolism of the organism, reduces glutamic-pyruvic transaminase and glutamic-oxaloacetic transaminase activities in the liver and improves the immune level of the organism, greatly solves the problem of protein feed resource shortage, reduces emission of urine nitrogen, fecal nitrogen and total nitrogen, guarantees food safety and reduces breeding pollution.

The application provides application of a TRPML1 specific small molecule inhibitor ML-SI3 in preparation of a medicine for treating or preventing nephritis. The application has the following beneficial effects: the TRPML1 specific small molecule inhibitor ML-SI3 can significantly reduce the serum anti-dsDNA antibody titer level of MRL / lpr lupus mice, reduce the urine albumin / creatinine ratio level, reduce the serum urea nitrogen level, reduce the swelling degree of the spleen and axillary lymph nodes, improve the kidney pathological changes, has a comprehensive protective effect on nephritis of MRL / lpr lupus mice, and provides application of a TRPML1 specific small molecule inhibitor ML-SI3 in preparation of a medicine for treating or preventing nephritis, provides a new medicine for treating lupus nephritis, and has good market value and clinical application prospect.

This invention discloses the application of Dendrobium huoshanense protocorms in anti-fatigue and kidney-tonifying and aphrodisiac effects. Combining Dendrobium huoshanense protocorms with Cistanche deserticola, American ginseng, Rubus idaeus, Cornus officinalis, and Lycium barbarum significantly enhances the anti-fatigue and kidney-tonifying and aphrodisiac effects of the formula. Compared with Cistanche deserticola alone, adding Dendrobium huoshanense protocorms significantly prolongs the exhaustive swimming time in mice, reduces serum urea nitrogen and lactic acid levels, increases liver glycogen content, and increases muscle glycogen content. Compared with Cistanche deserticola alone, adding Dendrobium huoshanense protocorms significantly increases serum superoxide dismutase activity, reduces serum malondialdehyde content, increases serum testosterone content, increases penile nitric oxide synthase activity, and increases penile nitric oxide and cyclic guanosine monophosphate content in penile tissue.

The present invention discloses a hexapeptide VA6 with anti-fatigue function, as well as its preparation method and application, belonging to the field of biotechnology. The hexapeptide VA6, screened from Pacific salmon sperm protein hydrolysate, has an amino acid sequence of VGVVVA and strong binding ability to AMPK and LDH. It can significantly increase the liver glycogen content and significantly reduce the serum urea nitrogen content in fatigue model mice, alleviate the fatigue phenotype of fatigue model mice, and significantly increase the weighted swimming time of fatigue model mice. It has anti-fatigue function and can be used in the development of functional foods that relieve fatigue.

The invention discloses a pharmaceutical composition for relieving physical fatigue as well as a preparation method and application thereof. The pharmaceutical composition is prepared from the following raw material medicines in parts by weight: 1-3 parts of cervus elaphus linnaeus, 1.6-3 parts of ginseng, 2-6 parts of fructus lycii, 2-6 parts of schisandra chinensis and 1-3 parts of tortoise shells. According to the preparation method disclosed by the invention, an extraction process of decocting the tortoise shells firstly and adding the ginseng and the like later is adopted, and a spray drying and 85% ethanol granulation technology is combined, so that active ingredients are effectively reserved. Pharmacodynamic experiment results show that the composition can significantly prolong the loaded swimming time of mice, improve the liver glycogen reserve and reduce the levels of blood lactic acid and serum urea nitrogen, and the effect is significantly superior to that of simple superposition of each single medicinal material. The traditional Chinese medicine composition is scientific in formula, remarkable in synergistic interaction, stable in process, high in safety and suitable for conditioning and relieving physical fatigue for a long time.

The invention discloses clostridium pralaense for improving the intestinal barrier function of a mouse with chronic kidney disease and application of the clostridium pralaense, and belongs to the technical field of microorganisms and the technical field of medicines. The clostridium prausnitzii CCFM1204 provided by the invention has beneficial effects in delaying the renal failure process, can significantly improve kidney pathological characteristics of mice with adenine-induced chronic nephropathy, reduce the serum urea nitrogen level, reduce the collagen volume fraction of kidney tissues, improve the intestinal barrier function, and can be used for preventing and treating the chronic nephropathy of the mice with adenine-induced chronic nephropathy, so that the clostridium prausnitzii CCFM1204 can be used for preventing and treating the chronic nephropathy of the mice with adenine-induced chronic nephropathy of the mice with adenine-induced chronic nephropathy. The serum LPS level and the expression quantity of proinflammatory factors in the colon tissue are reduced, and the expression quantity of tight junction proteins in the colon tissue is improved. The clostridium praeparatum CCFM1204 provided by the invention has a wide application prospect in preparation of drugs for relieving or improving intestinal barrier dysfunction related to chronic kidney diseases.

The invention provides application of pigeon pea extract and active ingredients in preparation of drugs or functional food for relieving hyperuricemia. Animal experiment research finds that intervention of pigeon pea extract and pinostrobin significantly reduces serum uric acid content of a hyperuricemia mouse, prevents serum creatinine and / or serum urea nitrogen in vivo from increasing, and can increase urine urea nitrogen and / or urine creatinine excretion; meanwhile, the pigeonpea extract and the pinostrobin can restore the kidney and liver coefficients of a hyperuricemia mouse to a normal level, remarkably relieve kidney injury caused by hyperuricemia, inhibit kidney inflammation / fibrosis reaction and restore the physiological state of the kidney to the level of a normal mouse.

The invention provides a traditional Chinese medicine composition for treating chronic renal failure as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition for treating chronic renal failure is prepared from the following raw materials: rheum officinale, polygonum cuspidatum, leonurus, astragalus membranaceus, dandelion, poria cocos, plantain herb, herba lycopi, white mulberry root-bark, radix paeoniae alba and eclipta. The traditional Chinese medicine composition adjusts traditional Chinese medicine components, follows the compatibility principle of monarch, minister, assistant and guide and the structure theory, reasonably organizes medicines, enhances the curative effect, reduces toxic and side effects, comprehensively takes care of the illness state, improves the treatment effect of the traditional Chinese medicine composition on chronic renal failure, adjusts the abnormal levels of serum urea nitrogen and serum creatinine, and improves calcium and phosphorus metabolism disorder.

This invention provides an anti-vertigo and / or anti-fatigue composition, its formulation, and its application, relating to the field of functional formulation technology. The composition comprises the following components in weight percentages: 0.01%-0.5% turmeric extract, 1%-5% branched-chain amino acids, 0.5%-3% ginger extract, and 0.05%-0.5% oyster peptides. Animal experiments have demonstrated that the composition of this invention can significantly reduce the vertigo response index in rats with motion sickness, prolong weight-bearing swimming time, reduce blood lactate and serum urea nitrogen levels, and increase liver glycogen reserves. Compared with commercially available anti-motion sickness chemical drugs such as dimenhydrinate, this invention achieves anti-vertigo effects without causing a decrease in weight-bearing swimming time and has significant anti-fatigue effects. The composition of this invention is composed of natural raw materials, has high safety, and achieves a synergistic effect of anti-vertigo and anti-fatigue, and can be widely used in the preparation of drugs or health products for the prevention and / or treatment of motion sickness and the relief of physical fatigue.

The invention discloses a pharmaceutical composition for treating chronic kidney disease stage 1-3 as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The composition consists of 40-60 parts of astragalus membranaceus, 40-60 parts of black soybeans, 8-12 parts of angelica sinensis, 24-36 parts of dogwood and 8-12 parts of rhodiola rosea. The preparation method comprises the following steps: pretreating the raw materials, soaking, boiling for several times and filtering to obtain a finished product. Based on the treatment principles of tonifying qi, activating blood, dredging collaterals and removing blood stasis in traditional Chinese medicine, the traditional Chinese medicine composition conforms to the pathogenesis of chronic kidney disease deficiency in root and excess in superficies, all the components synergistically play a role, the serum urea nitrogen, serum creatinine level and glomerular filtration rate of a patient can be improved and edema, weakness and other symptoms can be relieved by combining basic treatment, the total effective rate reaches 83.33%, and the traditional Chinese medicine composition is good in safety, easy and convenient to prepare and suitable for clinical application. The traditional Chinese medicine composition is suitable for long-term administration of patients with spleen and kidney qi deficiency and blood deficiency with stasis syndrome type chronic kidney diseases at 1-3 stages, and has good application value in treatment of related diseases.

The application discloses an anti-fatigue plant source nutritional supplement containing total paeony glycosides and a preparation method thereof. The nutritional supplement comprises a total paeony glycoside derivative modified by enzymatic selective acylation, vitamins and minerals, and a compound of acanthopanax senticosus extract, astragalus extract, medlar extract and grape seed extract. The total paeony glycoside derivative is selectively modified by introducing a C4-C8 medium-chain fatty acid acyl group to the C-6' hydroxyl group of paeonoside. The application significantly improves the fat solubility and membrane permeability of the total paeony glycoside by using a green and mild enzymatic acylation technology, so that the apparent permeability coefficient is increased by 8-12 times. The nutritional supplement prolongs the weight-bearing swimming time of mice by 92.8%, significantly reduces the serum urea nitrogen and blood lactic acid levels, increases the blood lactic acid elimination ratio to 37.8%, activates the AMPK-PGC1 alpha energy metabolism pathway and adjusts the central nervous transmitter 5-HT / DA ratio, and has a synergistic anti-fatigue effect.

The present application relates to the technical fields of food processing and functional active peptide development, and particularly relates to a small molecule active peptide of Agaricus bisporus and a preparation method and application thereof. Small molecule polypeptides are prepared by using alkaline protease and flavor protease directional complex enzymolysis on Agaricus bisporus dry powder, and the small molecule polypeptides are separated and purified by ultrafiltration and dextran gel G-15 column, and combined with liquid chromatography-mass spectrometry analysis to obtain small molecule weight Agaricus bisporus polypeptides with amino acid composition of PNPGHPP, SPDGHAPGGPP, FFPVHH and LPPPHDF. The obtained small molecule weight Agaricus bisporus polypeptides have dual effects of antioxidant and anti-fatigue, can effectively reduce serum urea nitrogen and lactic acid, liver malondialdehyde content, improve liver glycogen content and superoxide dismutase and glutathione peroxidase activity, and have wide application prospect in the fields of food and health care products.

The invention provides a precise nutrition regulation and control method for promoting intestinal development of young meat breeding chickens and application, belongs to the technical field of livestock and poultry breeding and production, and aims to solve the problem that hatching eggs laid by meat breeding chickens of 50 weeks old or above are insufficient in nutrition reserve, and L-methionine is injected through amniotic cavities when the meat breeding chickens are hatched to 17 embryonic ages. According to the method, the jejunum length, the jejunum weight and the ileum length (Plt: 0.05) of the 21-day-old offspring broiler chicken are remarkably increased, the expression of ileum proinflammatory factors (IL-8 and TNF-alpha) and apoptosis pathway genes (BAX, Caspase-3 and the like) is down-regulated, the expression of anti-apoptosis genes Bcl-2 is up-regulated, and the serum urea nitrogen level is reduced. Meanwhile, the daily average feed intake and the daily weight gain (Plt; 0.05) are improved. Through accurate intervention in the embryonic period, parent nutrition transfer limitation is broken through, the intestinal development and immune functions of offspring are improved, and an effective solution is provided for healthy breeding of offspring of elderly breeding hens.

This invention provides a method for constructing a rat model of hyperuricemia, belonging to the field of animal model construction technology. SPF-grade male rats were selected and, after adaptive feeding, were administered inosine (5-15 mg / kg), potassium oxonate (150-160 mg / kg), and hypoxanthine (100-200 mg / kg). Inosine and potassium oxonate were administered intraperitoneally, while hypoxanthine was administered subcutaneously. Experimental results showed that serum uric acid levels significantly increased one hour after modeling, and the hyperuricemic state could be maintained for up to 24 hours, significantly better than traditional models. Simultaneously, serum urea nitrogen and creatinine levels did not show significant increases, indicating that the model did not cause serious kidney damage. The hyperuricemia model constructed by this invention has a rapid onset of action, a long duration of hyperuricemia, and minimal kidney damage, making it suitable for the screening and evaluation of anti-hyperuricemia drugs.

The invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a preparation method and application of a rhizoma gastrodiae yin-tonifying oral preparation. The oral preparation is prepared from the following raw materials in parts by weight: 20 to 25 parts of rhizoma gastrodiae, 15 to 20 parts of radix rehmanniae, 14 to 17 parts of fructus corni, 17 to 21 parts of fructus lycii, 15 to 20 parts of hail, 10 to 15 parts of silver grass, 10 to 20 parts of radix ophiopogonis, 10 to 14 parts of radix polygonati officinalis, 5 to 10 parts of radix ginseng, 7 to 12 parts of rhizoma atractylodis macrocephalae, 5 to 10 parts of poria cocos, 10 to 15 parts of semen ziziphi spinosae, 5 to 10 parts of fructus schisandrae chinensis, 5 to 8 parts of radix angelicae, 1 to 5 parts of radix angelicae sinensis and 3 to 8 parts of liquorice root. Animal experiments prove that the oral preparation can prolong the exhaustive swimming time of mice, reduce the content of blood lactic acid and serum urea of the mice after swimming and increase the reserve of hepatic glycogen, and has a remarkable anti-fatigue effect.

The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a preparation method and application of a Gastrodia elata Yin-boosting oral preparation. The oral preparation comprises the following raw materials in parts by weight: Gastrodia elata 20-25 parts, Rehmannia glutinosa 15-20 parts, Cornus officinalis 14-17 parts, Fructus Lycii 17-21 parts, Rubus corchorifolius 15-20 parts, Anoectochilus roxburghii 10-15 parts, Ophiopogon japonicus 10-20 parts, Polygonatum odoratum 10-14 parts, Panax ginseng 5-10 parts, Atractylodes macrocephala 7-12 parts, Poria cocos 5-10 parts, Ziziphus jujuba var. spinosa 10-15 parts, Schisandra chinensis 5-10 parts, Angelica dahurica 5-8 parts, Angelica sinensis 1-5 parts, and Glycyrrhiza uralensis 3-8 parts. Animal experiments prove that the oral preparation can prolong the exhaustive swimming time of mice, reduce the content of blood lactic acid and serum urea of mice after swimming, increase the storage of liver glycogen, and has a significant anti-fatigue effect.

The invention provides a health-care nutrition regulator containing a kelp extract and a preparation process of the health-care nutrition regulator, and belongs to the technical field of health-care products. The health nutrition regulator comprises a kelp extract, an acanthopanax senticosus extract, a Chinese magnoliavine fruit extract, whey protein, galactooligosaccharide, beta-glucan, vitamin C, compound vitamin B and hydroxypropyl-beta-cyclodextrin, wherein the kelp extract is obtained by superfine grinding of kelp, ultrasonic enzymolysis of compound enzyme and purification. The kelp extract, the acanthopanax senticosus extract and the Chinese magnoliavine fruit extract have a synergistic effect, a multi-protection net capable of externally cleaning free radicals, internally activating enzyme activity and blocking oxidative damage is constructed, energy metabolism is adjusted through multiple target points, lactic acid removal is accelerated, and the synergistic interaction of fatigue resistance and oxidation resistance is achieved. Animal experiments show that the health-care nutrition regulator disclosed by the invention can be used for remarkably prolonging the loaded swimming time of mice and reducing the levels of lactic acid, serum urea nitrogen and creatine kinase, and shows a good synergistic effect.