76 results about "Serum urea" patented technology

The invention provides a stable serum urea detection method with a strong antijamming capability and reagents. The stable serum urea detection method with the strong antijamming capability adopts an enzyme coupling rate method, can effectively detect the content of serum urea, and has the advantages of strong antijamming capability, good stability and the like. The reagent comprises a reagent R1 and a reagent R2, and a new buffer system and a stabilizer are adopted, so that the stability of the reagents is remarkably improved. Meanwhile, a novel surfactant alkyl polyglucoside is adopted, so that not only is the measuring performance remarkably improved, but also the stability and the antijamming capability of the reagent are enhanced, and the stable serum urea detection method with the strong antijamming capability and the reagent can fully meet the clinical requirement.

The invention provides a traditional Chinese medicine composition for treatment of chronic renal diseases and chronic renal failure and its preparation method. The traditional Chinese medicine composition is prepared from the following raw material medicines by weight: 6-40 parts of rheum officinale, 10-95 parts of astragalus and 5-65 parts of salvia miltiorrhiza by a certain extraction and purification process into an injection, a freeze-dried powder injection or other oral preparations. Animal experiments confirm that, the traditional Chinese medicine composition can obviously improve the hemodynamic indexes of rats subjected to chronic glomerulonephritis, reduces serum IL-6, IL-8, and TNF (tumor necrosis factor)-alpha levels, significantly reduces serum creatinine and urea nitrogen and other indexes of rats subjected to hypertensive nephropathy, significantly reduces serum urea nitrogen and serum creatinine contents of rats subjected to the chronic renal failure, increases serum albumin content, and also can improve each hemodynamic index of rats subjected to the chronic renal failure; and pathological results show that the traditional Chinese medicine composition can improve and inhibit injures on kidney caused by the chronic renal diseases and chronic renal failure.

The invention discloses Lactobacillus plantarum WZMX-2 as well as application and prepared whey pear wine thereof. Bitter-free whey pear wine is prepared by the following steps: uniformly mixing whey fermentation liquor composed of Lactobacillus plantarum WZMX-2, Kluyveromyces marxianus WWMJ-1 and saccharomyces cerevisiae at the volume ratio of 4:4:2 with whey bergamot pear juice mixed liquor; culturing at 25-37 DEG C for 24-72 hours; and fermenting when measuring that the alcohol degree reaches 7.8% vol, the total acid reaches 60.84 titration acidity, the total sugar content reaches 42.34 g/L, and the bitter peptide content reaches 0.15 g/100 mL. The prepared whey pear wine is yellowish green, clarified and translucent, has natural color and fragrance of whey and pear, and has no bitter feeling; functional tests prove that the prepared whey pear wine can prolong weight-bearing swimming time of mice, reduces the generation of serum urea, increases the reserve of liver glycogen, has no apparent influence on weight and blood lactic acid of the mice, and has a functional effect of alleviating physical fatigue.

The invention relates to a construction method for an acute hyperuricemia tree shrew model. The method comprises the following steps: (1) selecting adult healthy tree shrew with body weight of 110 to 150 g and an age of 1 to 3 years; (2) respectively measuring fasting serum uric acid values of males and females of tree shrew selected in the step (1); (3) carrying out conventional breeding on the tree shrew selected in the step (1) and pretreating the tree shrew by grasping and releasing the tree shrew with hand; and (4) injecting the tree shrew treated in the step (3) with an oteracil potassium suspension so as to obtain the acute hyperuricemia tree shrew model, wherein the amount of the used oteracil potassium suspension is 40 mg/kg to 100 mg/kg on the basis of the body weight of the tree shrew. The model has typical disease symptoms, a quantity index has statistical significance, and serum urea nitrogen and serum creatinine values and histopathological results prove that such a model making dosage range is safe and does not result in influence on the liver and the kidney.

The invention provides an anti-fatigue healthcare product or pharmaceutical composition and a preparation method and application thereof. The healthcare product or the pharmaceutical composition is prepared from, by weight, 50-90 parts of acanthopanax root, 30-50 parts of glossy ganoderma, 30-50 parts of wolfberry fruit and 60-110 parts of green tea. The healthcare product or the pharmaceutical composition is capable of effectively reducing organism serum urea nitrogen and blood lactic acid content and obviously prolonging load swimming time and has a good anti-fatigue effect. Besides, the healthcare product or the pharmaceutical composition is capable of increasing radiated mouse SOD (superoxide dismutase) activity, increasing white blood cells, lowering marrow micronucleus rate and increasing animal survival rate, has a remarkable anti-radiation function and is safe, non-toxic, good in taste and suitable for long-term taking.

The invention discloses an extract of an active anti-fatigue part of okra, and a preparation method and application thereof. The extract of the active anti-fatigue part of okra uses total polyphenols as a main active anti-fatigue component, wherein the total polyphenols account for 11.79 to 23.34% of the total mass of the extract of active anti-fatigue part and mainly comprise catechin derivatives and quercetin derivatives, and the quercetin derivatives are mainly composed of quercetin-3-O-gentiobioside, quercetin-3-O-glucose-xyloside, isoquercitrin, quercetin-3-O-malonyl-glucoside, rutin and quercetin. The extract of the active anti-fatigue part of okra provided by the invention can substantially prolong the load swimming time of mice, eliminate lactic acid accumulation after sports, reduce the content of serum urea nitrogen, increase hepatic glycogen and improve the antioxidation capability of mice in a stress state, and has substantial anti-fatigue effect.

The invention discloses a lycium barbarum polysaccharide-deer blood composition having functions of relieving fatigue and tonifying yang. Lycium barbarum polysaccharide and a deer blood dry powder asactive components are made into an oral preparation in accordance with conventional excipients and processes of the pharmaceutics, or are made into a food prepared for a meal in accordance with a health-care food process. Experiments show that the composition can significantly prolong the loaded swimming time of small mice, reduce the content of blood lactic acid and serum urea nitrogen after thesmall mice do exercise and increase the reserve amount of hepatic glycogen; compared with the fatigue relieving function of the single use of lycium barbarum polysaccharide and deer blood, the function is more comprehensive, and the effect is more effective. The composition also can significantly enhance the gonadal hormone level of rats and the activity of superoxide dismutase in testis tissues,reduces the content of malondialdehyde, enhances the antioxidant capacity of the rat, and has stronger activity than the activity of lycium barbarum polysaccharide and deer blood groups. Therefore, the composition can be used for enhancing the anti-fatigue and yang-tonifying ability of sub-health young and middle-aged men, as well as conditioning middle-aged and elderly patients with physical deficiency.

The invention relates to the field of medicines, in particular to application of an anoectochilus roxburghii polysaccharide extract. The anoectochilus roxburghii polysaccharide extract is used for preparing an anti-fatigue medicine, the anoectochilus roxburghii polysaccharide extract has obvious anti-fatigue effect and can obviously prolong weight-loading swimming time of a mouse, lower serum lactate dehydrogenase level after swimming, lower serum urea nitrogen content, improve the oxidation resistance of the mouse in a stress state and increase content of hepatic glycogen and muscle glycogen.

The invention provides a serum urea measuring reagent, and applications thereof. The serum urea measuring reagent comprises a reagent R1 and a reagent R2; the reagent R1 comprises following raw materials: Capso, NADH, and EDTA.Na2; and the reagent R2 comprises following raw materials: HEPES, alpha-ketoglutaric acid, urase, glutamate dehydrogenase, EDTA.Na2, a reducing agent, and BSA. Reagent formula is optimized, and buffer pH is adjusted, so that effective buffering capacity is provided, NADH stability is improved, tool enzyme oxidation is reduced, component synergistic interaction and effectimprovement are achieved, a problem in the prior art that blank absorbance change rate stability is poor is solved, hospital clinical detection results are reliable, the application prospect is promising, and the market value is extremely large.

The invention discloses a traditional Chinese medicine composite used for releasing fatigue as well as preparation method of the traditional Chinese medicine composite. The traditional Chinese medicine composite disclosed by the invention takes Maca which has various functions of improving fertility, resisting fatigue, improving sexual performance, antioxidation, inhibiting hyperplasia of prostate, alleviating menopausal syndrome, strengthening immunity combined with American ginseng extract, rhizome chuanxiong extract, morinda officinalis extract, tuber onion seed extract and tangerine peel extract. The traditional Chinese medicine compound has the functions of antioxidation and antifatigue, can lower the levels of lactate dehydrogenase and serum urea, improve succinate dehydrogenase activity of muscles, effectively relief uncomfortable feeling after exercise, such as limb weakness, tiredness and muscle soreness. The invention also provides a traditional Chinese medicine preparation containing the traditional Chinese medicine composite and a preparation method of the traditional Chinese medicine preparation.

The invention discloses a traditional Chinese medicine composition used for alleviating fatigue, and a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 30-50 parts of poria, 20-40 parts of rhizoma dioscoreae, 10-30 parts of fructus lycii, 10-30 parts of rhizoma polygonati, 10-30 parts of rhizoma polygonati odorati and 5-20 parts of ginseng radix. An animal function experiment indicates that the traditional Chinese medicine composition can prolong the loaded-swimming time of a mouse, the loaded-swimming time of mice in each experiment group is longer than the loaded-swimming time of mice in a control group, the content of blood lactic acid and the level of serum urea nitrogen in each experiment group are smaller than the content of the blood lactic acid and the level of serum urea nitrogen in the control group, and the traditional Chinese medicine composition has a function of alleviating physical power fatigue.

The present invention relates to a new use of citral for manufacturing a medicament for treating focal segmental glomerulosclerosis (FSGS). Particularly, the present invention discloses that citral is effective in alleviating symptoms of FSGS, including reducing glomerular epithelial hyperplasia lesions (EPHLs), peri-glomerular inflammation or glomerular hyalinosis or sclerosis, and also reducing proteinuria or hematuria or lowering serum urea nitrogen level or serum creatinine level in the subject.

The invention discloses health-care food with an anti-fatigue effect and a preparation method thereof. The health-care food is prepared from the following raw materials in part by weight: 300 to 450 parts of lophatherum gracile Brongn and 250 to 400 parts of American ginseng. A health-care product preparation provided by the invention has the advantages of obviously prolonging the burden swimming time of mice, reducing the production of serum urea nitrogen of the mice, reducing area under curve of blood lactate after the motion of the mice along with the anti-fatigue health-care effect. The health-care food adopts medicinal and edible natural plants as main raw materials, so that the preparation process and the product quality is stable and controllable; the curative effect is obvious; the provided formulation is convenient to administrate; and the health-care food is suitable for all kinds of people to eat.

The invention relates to a preparation method and application of a compound polygonum multiflorum preparation, belonging to the technical field of research and development of medical products. The preparation method of the compound polygonum multiflorum preparation comprises the following steps of: selecting 6-9 grams of high-quality polygonum multiflorum, 6-9 grams of angelica, 6-9 grams of gen-seng, 6-9 grams of acanthopanax, 6-9 grams of rhizoma polygonati, 4-6 grams of pericarpium citri reticulatae, , 4-6 grams of yams, 2-4 grams of umbellate pore fungus, 2-4 grams of hawthorns, 2-4 grams of malts and 2-4 grams of dates, and cleaning; carrying out microwave drying; crushing into powder of 50 meshes; adding water for boiling, and extracting for three times; merging extracting solutions; filtering to obtain filter liquor; concentrating the filter liquor into extractum; uniformly mixing the extractum with starches according to the proportion of 1:4; granulating by using a sieve of 10 meshes; drying, and then sieving by using a sieve of 14 meshes to obtain the compound polygonum multiflorum preparation. The product has the effect of reducing the content of the serum urea nitrogen of a fancy carp and is beneficial to industrialized production.

The invention provides a traditional Chinese medicinal preparation for treating chronic renal disease and a preparation method thereof. The traditional Chinese medicinal preparation is prepared from the following crude medicines in parts by weight: rheum officinale and safflower on (1-10):(10-1); the crude medicines are prepared into an injection, a freeze-dried powder injection or other oral preparations through certain extraction and purification processes. Animal experiments prove that the traditional Chinese medicinal preparation can be used for significantly improving the hemorheology indexes of mice infected by chronic glomerulonephritis and reducing serum IL-6, IL-8 and TNF-alpha levels; the preparation can be used for remarkably reducing serum urea nitrogen and serum creatinine contents of mice of chronic renal failure and increasing a serum albumin content; moreover, the preparation can be used for improving the various hemorheology indexes and levels of mice of chronic renal failure; a pathological result shows that the traditional Chinese medicinal preparation disclosed by the invention can be used for improving and inhibiting damage of chronic kidney disease and chronic renal failure on kidney; furthermore, the traditional Chinese medicinal preparation prepared by the optimal preparation method disclosed by the invention is more excellent than a traditional Chinese medicinal preparation prepared by a conventional method.

The refreshing musk preparation for treating ischemic cerebral diseases is prepared with the medicine powder of leech, ground beetle, artificial bezoar, nux vomica seed, pearl, ginseng, natural musk and borneol in certain proportion, and through mixing through stirring in some container and capsulizing or tabletting to make capsule or tablet. By means of the synergistic effects of the medicine components, the refreshing musk preparation can improve body's micro circulation, improve cardiac and cerebral ischemia, anoxia and hemerheological state, increase nourishing blood flow rate, reduce blood fat, lipofuscin, serum urea nitrogen and creatinie effect, eliminate arteriosclerosis block, resist thrombosis and platelet aggregation, and eliminate pathological change damage.

The invention provides a traditional Chinese medicine composition for treatment of chronic renal diseases and chronic renal failure and its preparation method. The traditional Chinese medicine composition is prepared from the raw material medicines of rheum officinale and salvia miltiorrhiza in a weight ratio of 1-10:10-1 by a certain extraction and purification process into an injection, a freeze-dried powder injection or other oral preparations. Animal experiments confirm that, the traditional Chinese medicine composition can obviously improve the hemodynamic indexes of rats subjected to chronic glomerulonephritis, reduces serum IL-6, IL-8, and TNF (tumor necrosis factor)-alpha levels, significantly reduces serum urea nitrogen and serum creatinine contents of rats subjected to the chronic renal failure, increases serum albumin content, and also can improve each hemodynamic index of rats subjected to the chronic renal failure; pathological results show that the traditional Chinese medicine composition can improve and inhibit injures on kidney caused by the chronic renal diseases and chronic renal failure; and the treatment effect of the traditional Chinese medicine preparation prepared by the preparation method provided by the invention is better than that of a traditional Chinese medicine preparation prepared by a conventional method.

The invention discloses a composition for remarkably improving the anti-fatigue function, and a preparation method and an application thereof. The composition comprises, by weight, 24-48 parts of a Haematococcus pluvialis solid dispersion, 900-2500 parts of Lepidium meyenii Walp, 100-600 parts of taurine and 140-320 parts of acai berry powder, wherein the mass percentage content of astaxanthin in the Haematococcus pluvialis solid dispersion is 2.0-3.0%, the Lepidium meyenii Walp undergoes heat refluxing extraction by using ethanol to prepare extract containing 0.02-0.05% by mass of macamides and 5-9% by mass of polysaccharides, and the acai berry powder is prepared through pulping, filtering for residual removal, and spray drying of fresh acai berries. A test result shows that the composition can remarkably prolong the load swimming time of load swimming mice, obviously improve the liver starch level of the mice and remarkably reduce the area under the blood lactic acid curve and the serum urea nitrogen content of the mice after the swimming, and has a good physical fatigue alleviation effect.

The invention provides an experiment analysis method for blood glucose reducing efficacy of Shenlian decoction rapidly disintegrating tablets for reducing blood glucose and belongs to the technical field of traditional Chinese medicine research. The method comprises the following steps of step one, selection of experiment grouping; step two, detection of physiological indicators, including detection of mice fasting blood-glucose, total cholesterol and triglyceride, detection of glucose tolerance, detection of urine volume, urine protein and urinary albumin, detection of serum creatinine and serum urea nitrogen, detection of serum sodium and serum potassium, detection of urine sodium and urine potassium and detection of weight and kidney indexes; step three, statistics of pathology; step four, analysis of statistical results; according to the experiment analysis method, an effective constituent ginseng total saponin and coptis total alkaloid compound (RH for short) of the Shenlian decoction rapidly disintegrating tablets for reducing blood glucose can effectively reduce the blood glucose level of a diabetic patient and has a certain therapeutic effects for early diabetic nephropathy.

The ribose oral liquid is water solution of D-ribose in distilled water and has D-ribose content of 35-40 wt%. The present invention is one kind of high-tech bioengineering product. Functional experiment shows that the product can prolong mouse's weight-loading swimming time and rod clamping time and reduce mouse's serum urea and glycogen consumption and has obviously anti-fatigue effect. It contains no any hormone and has no toxic side effect.

The invention relates to an application of 5' nucleotide in preparation of medicines and health-care foods for alleviating physical fatigue. The nucleotide is 5' nucleotide mixture which takes ribonucleic acid (RNA) as raw material, produced by enzymic degradation, has the purity of more than 99%, and is prepared by mixing 5' AMP, 5' CMP, 5' GMPNa2 and 5' UMPNa2 according to the mixture ratio of 22.8:26.6:30.2:20.4. The nucleotide can reduce and prolong the mouse burden swimming time, decreases the generation of blood lactate after movement, lowers the serum urea nitrogen level and has the function of alleviating physical fatigue.

The invention discloses a method for researching the anti-fatigue action of oligopeptides of Apostichopus japonicus Liao on mice and relates to the technical field of biology. Detecting the molecularweight of oligopeptides of the Apostichopus japonicus Liao firstly; performing grouping for an experiment and selecting gavage dose: 240 male Kunming mice weighing 20 g plus/minus 2 g are selected anddivided randomly into 8 groups, 30 mice are contained in each group in which 10 mice are used for loaded swimming, 10 mice are used for determination of serum urea nitrogen and 10 mice are used for determination of hepatic glycogen; performing a loaded swimming experiment; determining serum urea nitrogen; determining hepatic glycogen: 30 min after last gavage, the mice are killed with cervical dislocation, and livers are rinsed with normal saline, dried by filter paper and determined; after deep processing such as enzymolysis and the like on the Apostichopus japonicus Liao, oligopeptides withthe molecular weight smaller than 1000 Da are obtained. The anti-fatigue degree of oligopeptides of the Apostichopus japonicus Liao is compared with anti-fatigue degrees of soybean oligopeptides andApostichopus japonicus Liao powder, scientific basis is provided for access of oligopeptides of the Apostichopus japonicus Liao to market, and foundation is laid for development, utilization and popularization of novel marine drug resources.

The invention relates to an anti-fatigue ethanol extract of floriferous bacopa monnieri, a method for preparing the anti-fatigue ethanol extract and application thereof. The anti-fatigue ethanol extract, the method and the application have the advantages that anti-fatigue effects of the floriferous bacopa monnieri which is traditional Chinese medicine are observed via loaded-swimming fatigue models of mice, as proved by results, the loaded-swimming time of the mice can be obviously prolonged by the extract, the contents of blood lactic acid and serum urea nitrogen in the bodies of the mice can be reduced after the mice exercise, reserved hepatic glycogen further can be increased, and the exercise tolerance can be improved; the anti-fatigue effects can be realized by the extract of the floriferous bacopa monnieri, the fatigue degrees of human bodies can be obviously relieved by the extract, and the extract can be used for producing and processing food or healthcare products.

The invention relates to applications of macrolide antibiotics, especially Erythromycin, in preparation of anti-fatigue disease medicines. The anti-fatigue function of macrolide antibiotics, especially Erythromycin is discovered firstly, animal test results show that macrolide antibiotics can prolong swimming time of mice bearing load greatly, lower the serum urea ammonia level after strenuous exercise, inhibit rise of blood lactic acid, keep or increase the content of ferrohemoglobin, raise lactate dehydrogenase activity, raise liver starch reserve, reduce liver starch consumption during exercise, and have functions of alleviating fatigue and increasing exercise endurance greatly.

The invention provides a traditional Chinese medicine composition for treatment of chronic renal diseases and its preparation method and use. The traditional Chinese medicine composition is prepared from the raw material medicines of rheum officinale, astragalus and safflower with a weight ratio of 3-45:9-85:3-65 by a certain extraction and purification process into an injection, a freeze-dried powder injection or other oral preparations. Animal experiments confirm that, the traditional Chinese medicine composition can obviously improve the hemodynamic indexes of rats subjected to chronic glomerulonephritis, reduces serum IL-6, IL-8, and TNF (tumor necrosis factor)-alpha levels, significantly reduces serum creatinine and urea nitrogen and other indexes of rats subjected to hypertensive nephropathy, significantly reduces serum urea nitrogen and serum creatinine contents of rats subjected to chronic renal failure, increases serum albumin content, and also can improve each hemodynamic index of rats subjected to the chronic renal failure; and pathological results show that the traditional Chinese medicine composition can improve and inhibit injures on kidney caused by the chronic renal diseases and chronic renal failure.