56 results about "Streptomyces diastatochromogenes" patented technology

The invention discloses a preparation method of microbial pesticide for controlling vegetable fungal diseases, belonging to the microbial technical field, which utilizes a streptomyces diastatochromogenes D isolated and screened from a soil sample to prepare various formulation pesticides with the steps of the activation cultivation of strain, fermentation cultivation, leavening extraction and distribution of pesticide auxiliary material to the purified active composite, wherein the soil sample is acquired from Lin' an Tianmu mountain in Hangzhou, Zhejiang. The preparation method of microbial pesticide for controlling vegetable fungal diseases is safe and can be used for controlling cucumber fusarium wilt, tomato gray mold, and other vegetable fungal diseases; and the controlling effect is a little better than normal chemical agents.

The invention discloses a preparation method of a microbial pesticide for controlling vegetable fungus diseases, belonging to the technical field of microorganisms. The preparation method comprises the steps of: activating and culturing strains of streptomyces diastatochromogenes D obtained by the separation and screening of soil samples acquired from Tianmu Mountain in Lin'an, Hangzhou Zhejiang;fermenting and culturing; extracting fermented materials; and preparing the purified active compounds by matching with agricultural pesticide auxiliary materials, and the like into various formationsof agricultural pesticides. The microbial pesticide can be used for controlling vegetable fungus diseases of cucumber fusarium wilt, tomato gray mold or tomato early blight, and the like, and has control effect which is equal or slightly superior to the traditional chemical pesticide, and is safe.

The invention relates to the technical field of microorganisms, particularly to a Streptomyces diastatochromogenes D metabolite and imidacloprid mixed formula preparation. Streptomycesdiastatochromogenes is named Streptomycesdiastatochromogenes D. The strain is preserved in the CGMCC (China General Microbiological Culture Collection Center) of the China Committee for Culture Collection of Microorganisms on May25th, 2007 with the preservation register number of CGMCC No.2060. Metabolites capable of inhibiting nilaparvatalugensstal yeast symobiotic bacteria can be obtained through fermentation and extraction of the Streptomyces diastatochromogenes D. The most significant characteristic of the preparation is that the Streptomyces diastatochromogenes D metabolites and conventional insecticide imidacloprid are mixed in a certain proportion to prepare a new formula, after the mixed formula is used for controlling nilaparvata lugens stal, the usage amount of insecticides can be reduced obviously, the control effect on the nilaparvata lugens stal can be effectively improved, and the development of the drug resistance of the nilaparvata lugens stal can be delayed.

The invention discloses an application of tetramycin P in inhibition of rhizoctonia solani kuhn of cucumbers and botrytis cinerea, belonging to the microbial technical field. An active compound tetramycin P is separated and purified by virtue of extraction and chromatography according to a conventional chemical method by carrying out activated culture of a strain, fermental cultivation and fermentation broth collection on streptomyces diastatochromogenes D which is separated and screened from a soil sample which is collected in Tianmu Mountains, Linan, Hangzhou, Zhejiang province. The compound is a novel tetar-alkene macrolide antibiotic, can be used for inhibiting growth of pathogenic fungi of crops such as rhizoctonia solani kuhn of cucumbers and botrytis cinerea and is stronger in inhibitory effect than a common chemical agent.

The invention discloses a Streptomyces diastochromogenes, a fermentation product 5'-epi-SPA-6952A and an application thereof. The Streptomyces diastochromogenes of the invention has the preservation number of CGMCCNo.2281, the fermentation product thereof has favourable insecticidal activity, and the active ingredient with insecticidal activity in the fermentation product is 5'-epi-SPA-6952A. Thefermentation liquor and the active ingredient 5'-epi-SPA-6952A of the Streptomyces diastochromogenes of the invention both have favourable insecticidal activity and low production cost, is friendly to environment and has favourable application prospect. In addition, the compound 5'-epi-SPA-6952A is a new compound, thus having wide development and application prospect.

The invention discloses a preparation method of microbial pesticide for controlling fungal diseases of vegetables and belongs to the technical field of microorganisms. The method comprises the following steps: activation and culture of Streptomyces diastatochromogenes strain D which is isolated from soil in Tian Mu Mountain in Lin'an of Hangzhou, Zhejiang province; fermentation culture; culture extraction; and combination of extracted active compound with agricultural adjuvant. The microbial pesticide is available in various forms. The microbial pesticide is used to control fungal diseases such as cucumber fusarium wilt and tomato gray mold, and is superior in fungicidal effect and safety to the common chemical pesticides.

The invention discloses an application of toyocamycin in prevention and control of cucumber rhizoctonia rot, which belongs to the technical field of microorganism. The method comprises the following steps: performing strain activation culture on streptomyces diastatochromogenes separated and screened from the soil samples collected from Tianmu Mountain, Lin'an, Hangzhou, Zhejiang; performing fermentation culture; extracting the fermented product; and separating and purifying the fermented product to obtain the active compound toyocamycin. Pot experiments indicate that the toyocamycin has obvious treatment and protection effect on cucumber rhizoctonia rot and better prevention and control effect than the conventional chemical agents, while ensuring safety.

The invention relates to the technical field of microbiology, particularly to an application of a mixed formula preparation to control on nilaparvata lugens stal. Streptomyces diastatochromogenes is named streptomyces diastatochromogenes D. The strain is preserved in the CGMCC (China General Microbiological Culture Collection Center) of CCCCM(China Committee for Culture Collection of Microorganisms)on May 15th, 2007, and the preservation registration number is CGMCC No.2060. Metabolic products having an inhibition effect on nilaparvata lugens stal yeast-likesymbiotic bacteria can be obtained throughfermentation and extraction of the streptomyces diastatochromogenes D.The application is most significantly characterized in that the metabolic products of the streptomyces diastatochromogenes D and imidacloprid are mixed in certainproportion to prepare a new formula, the usage amount of a pesticide can be obviously reduced when the mixed formula is used for controlling the nilaparvata lugens stal, the control effect on the nilaparvata lugens stal can be effectively improved, and the development of the drug resistance of the nilaparvata lugens stal can be delayed.

The invention discloses a compound with bacteriostatic activity as well as a preparation method and an application thereof. The compound with antibacterial activity is obtained by separating and purifying a product obtained by specific fermentation culture of streptomyces diastatochromogenes D. A preservation number of the Streptomyces diastatochromogenes D is CGMCC No. 2060. The streptomyces diastatochromogenes D disclosed by the invention is fermented and extracted to obtain an active compound Tetramycin Z with an inhibiting effect on part of plant fungal disease pathogenic bacteria, and thecompound has a relatively strong inhibiting effect on common plant pathogenic fungi; and the compound provides a new approach for development of new agricultural bactericides in the future.

The invention discloses a method for improving output of tetraene macrolide antibiotics and belongs to the technical field of microorganisms. According to the method, 100mu g / mL paromomycin is added in a solid medium which is used for culturing Streptomyces diastatochromogenes D to obtain a Streptomyces diastatochromogenes mutant strain which has relatively good resistance to the paromomycin; the capacity of synthesizing the tetraene macrolide antibiotics of the mutant strain is stronger than that of the Streptomyces diastatochromogenes D.

The invention discloses an S.diastatochromogenes electroporation transformation method, which comprises: carrying out a mild wall breaking treatment on cells, and carrying out electroporation transformation, wherein the mild wall breaking treatment adopts an electroporation buffer solution containing 5 mug / ml of lysozyme, or adopts an electroporation buffer solution containing 1% glycine, or adopts an electroporation buffer solution containing 30 mug / ml of penicillinG, or adopts an electroporation buffer solution containing 3 mug / ml of lysozyme and 20 mug / ml of penicillinG, and the electroporation transformation parameters comprise an electric field intensity of 12 kV / cm, a cell concentration of 1*10<9> / ml, and a plasmid DNA concentration of 2 mug; and adopting a YEME culture medium to incubate after electroporation transformation, wherein an incubation time is 2-3 h. According to the present invention, a convenient and reliable method is provided for efficient transformation of S.diastatochromogenes, and a path is paved for S.diastatochromogenes gene engineering.

The invention discloses a preparation method of microbial pesticide for controlling vegetable fungal diseases, belonging to the microbial technique field, which utilizes a streptomyces diastatochromogenes D isolated and screened from a soil sample to prepare various formulation pesticides with the steps of the activation cultivation of strain, fermentation cultivation, leavening extraction and distribution of pesticide auxiliary material to the purified active composite, wherein the soil sample is acquired from Lin' an Tianmu mountain in Hangzhou, Zhejiang. The preparation method of microbial pesticide for controlling vegetable fungal diseases is safe and can be used for controlling cucumber fusarium wilt, tomato gray mold, and other vegetable fungal diseases; and the controlling effect is a little better than normal chemical agents.

The invention relates to a genetically engineered strain Streptomyces diastatochromogenes CATF with high yield of epsilon-polylysine, and a method for improving the fermentation level of the epsilon-polylysine. A construction method of the high-producing gene genetically engineered strain comprises the following steps: step 1, constructing a plasmid for expressing acetyl-CoA acetyltransferase CATFgene, wherein a gene CATF sequence fragment is controlled by an erythromycin promoter erm* on the pIMEP plasmid; and step 2, acquiring a strain expressing the catF gene, namely the genetically engineered strain Streptomyces diastatochromogenes CATF with high yield of the epsilon-polylysine. Experiments prove that the epsilon-polylysine production capacity of the genetically engineered streptomyces strain is remarkably improved by 27.91% compared with that of an original strain Streptomyces diastatochromogenes TUST under the same condition, and an excellent strain is provided for epsilon-polylysine production.

The invention discloses a microbial pesticide for preventing and treating fungal diseases. The microbial pesticide comprises the following materials in parts by weight: 22-40 parts of verticillium lecanii, 5-17 parts of corn flour, 11-20 parts of polyisobutylene succinimide, 25-36 parts of malic acid, 15-32 parts of diatomite, 3-7 parts of metarhizium powder, 15-30 parts of trichoderma harzianum, 5-19 parts of amino-oligosaccharin, 6-9 parts of beta-cypermethrin, 45-62 parts of streptomyces diastatochromogenes D and 15-18 parts of arteannuin. The microbial pesticide is applied to preventing and controlling fungal diseases of plants and is similar with or slightly superior to general chemical agents in effect, and a new way is provided for the development of agricultural fungicides.

The invention relates to a genetically-engineered strain streptomyces diastatochromogenes with high production of epsilon-polylysine. The streptomyces diastatochromogenes is obtained by over-expressing three key genes, in different metabolic pathways, of a succinate dehydrogenase gene sdhB, a lysine / ornithine decarboxylase gene dcdA and an asparagine synthetase gene asnO in streptomyces diastatochromogenes TUST separately. According to the genetically-engineered strain streptomyces diastatochromogenes with high production of the epsilon-polylysine, by over-expressing the key genes in the different metabolic pathways, the genetically-engineered recombinant strain is obtained, and an experiment proves that the epsilon-polylysine-producing capability of the streptomyces genetically-engineeredstrain is improved compared with that of the original strain streptomyces diastatochromogenes TUST in the same situation, so that the excellent strain is provided for production of epsilon-polylysine.

The invention discloses a bacterial strain for increasing the yield of tetramycin Z and a method for preparing tetramycin Z by using the bacterial strain. The strain is classified and named as Streptomyces diastatochromogenes tz3-1 and is preserved on December 3, 2019, with an accession number being CGMCC No. 19069. The invention further discloses a preparation method of the streptomyces diastatochromogenes tz3-1. According to the streptomyces diastatochromogenes tz3-1 disclosed by the invention, the content of tetramycin Z in fermentation liquor obtained by fermenting the Streptomyces diastatochromogenes tz3-1 is 20.6 times higher compared with control Streptomyces diastatochromogenes D, so a foundation is laid for industrial production of tetramycin Z in the future.

The invention discloses a preparation method of a pesticide for preventing and treating vegetable fungal disease microorganisms, belonging to the technical field of microorganisms. The method comprises the following steps of: activating and culturing strains of streptomyces diastatochromogenes D which is separated and sifted from a soil sample collected from Tianmu Mountain in Linan, Hangzhou, Zhejiang in China; fermenting and culturing; extracting leavening; and mixing agricultural pesticide auxiliary materials with a purified active compound to prepare the agricultural pesticide in a plurality of formulations. The microorganism agricultural pesticide can be used for preventing and treating vegetable fungal diseases, such as cucumber fusarium wilt, tomato gray mold or tomato early blight, and the like, and is safe; and the preventing and treating effect of the agricultural pesticide are equivalent to or slightly better than that of the traditional chemical agents.

The invention discloses an frr expression reinforced recombined streptomyces diastatochromogenes, as well as a construction method and use. The recombined streptomyces diastatochromogenes excessively expresses ribosome cycle factor frr and has higher toyocamycin synthesis capability than streptomyces diastatochromogenes 1628. The construction process is as follows: 1) constructing an expression vector pIB139-frr; and 2) utilizing a conjugational transfer method to integrate the expression vector with the chromosome of the streptomyces diastatochromogenes so as to obtain the engineering bacteria. A promoter permoE* on the pIB139 vector is utilized to start expression of frr gene; the vector pIB139-frr is specifically integrated with the chromosome of the streptomyces diastatochromogenes 1628 by the conjugational transfer method so as to obtain engineering bacteria with genetic stability. Compared with the original strain, the engineering bacteria obviously improves the transcriptional level of the key enzyme gene toyF in the recombined toyocamycin synthesis route, increases the yield of the toyocamycin by at least 43.6%, and shortens the fermentation period by 12h.

The invention discloses a method for improving the capacity of Streptomyces diastatochromogenes for synthesizing toyocamycin and belongs to the technical field of microbes. According to the method, 10 mug / mL of erythromycin is added to a solid medium for culturing Streptomyces diastatochromogenes D, a Streptomyces diastatochromogenes mutant strain with higher resistance to the toyocamycin is obtained, and the capacity of the mutant strain for synthesizing the toyocamycin is higher than that of the Streptomyces diastatochromogenes D.

The invention discloses a recombinant streptomyces diastatochromogenes with reinforced adpA expression, a construction method and an application. A transcriptional regulation factor adpA at a secondary metabolism network center is excessively expressed, and the recombinant streptomyces diastatochromogenes has higher toyocamycin synthesis capacity compared with streptomyces diastatochromogenes 1628. The construction method comprises the following construction processes: (1) constructing an expression vector pIB139-adpA; and 2) merging the expression vector into a chromosome of the streptomyces diastatochromogenes by a conjugal transfer method so as to obtain engineering bacteria. The expression of adpA gene is started by utilizing a promoter permE* on the pIB139 vector, and the vector pIB139-adpA is specifically merged into the chromosome of the streptomyces diastatochromogenes 1628 by the conjugal transfer method so as to obtain the engineering bacteria with genetic stability. Compared with the original strain, the recombinant streptomyces diastatochromogenes has the advantages that the transcriptional level of a key enzyme gene toyF in the synthetic route of recombinant bacteria toyocamycin is obviously strengthened, and the yield of toyocamycin is improved by at least 31.6%. A foundation is laid for further improving the yield of toyocamycin and realizing industrial production as soon as possible.

The invention discloses a streptomyces diastatochromogenes engineering strain for producing toyocamycin, as well as a construction method and application thereof. Vitreoscilla hemoglobingene (vgb) is integrated on a chromosome of the streptomyces diastatochromogenes engineering strain, so that the toyocamycin expression capability is higher than that of streptomyces diastatochromogenes 1628. The construction process is as follows: 1) constructing an expression vector pIB139-vgb; and 2) utilizing a conjugation method to integrate the expression vector into the chromosome of streptomyces diastatochromogenes to obtain the engineering strain. A promoter permE* on the pIB139 vector is utilized to start the expression of the vgb gene, and the conjugation method is utilized to integrate the specificity of the vector pIB139-vgb into the chromosome of the streptomyces diastatochromogenes 1628 to obtain the engineering strain with stable inheritance. Compared with an original strain, the yield of the recombinant toyocamycin is at least improved by 21.2%, and the concentration of the strain is improved by 11.6%.

The invention discloses a method for increasing yield of toyocamycin through screening of united drug-resistant mutant strains and belongs to the field of microbial technologies. According to the method, Streptomyces diastatochromogenes D mutant strains generating drug resistance to low-concentration streptomycin, high-concentration streptomycin and paromomycin are obtained through screening of the united drug-resistant mutant strains, and the toyocamycin productivity of the obtained mutant strains is remarkably improved when compared with that of Streptomyces diastatochromogenes D.

The invention discloses application of streptomyces diastatochromogenes metabolite in prevention and treatment of black spot and anthracnose of Zhejiang ophiopogon japonicus, and relates to the technical field of microorganisms. The strain disclosed by the invention is classified and named as Streptomyces diastatochromogenes, the serial number is tz3-1, the preservation date is December 3rd, 2019, and the preservation registration number is CGMCC No.19069. The most significant characteristic of the invention is that the liquid fermentation metabolite of the strain can inhibit the growth of Zhejiang ophiopogon japonicus anthracnose pathogenic bacteria Colletotrichum liriopes and black spot pathogenic bacteria Alternaria alternata hypha at the same time. Field trials show that the effect of the bacterial strain metabolite on prevention and treatment of black spot and anthracnose of Zhejiang ophiopogon japonicus is slightly better than that of conventional chemical pesticides.

The invention relates to a gene engineering strain streptomyces diastatochromogenes for high yielding of epsilon-polylysine. The construction of the gene engineering strain comprises the following steps of: step 1, constructing plasmids for expressing the aspartokinase gene ask or the dihydropyridine dicarboxylic acid synthetase gene dhdps, wherein the gene ask or dhdps is controlled by an erythromycin promoter ermE * on pIMEP plasmids; and step 2, obtaining a strain for expressing the ask or dhdps gene, namely the gene engineering strain streptomyces diastatochromogenes for high yielding of epsilon-polylysine. Experiments prove that the epsilon-polylysine production capacity of the streptomyces gene engineering strain is remarkably improved by 17.2%-25.8% compared with that of the original strain streptomyces diastatochromogenes TUST under the same condition, and an excellent strain is provided for epsilon-polylysine production.

The invention provides streptomyces diastatochromogenes with high yield of toyocamycin in genetic engineering as well as a construction method and application of the streptomyces diastatochromogenes. According to the method, a recombinant plasmid pIB139-RelA is constructed by a genetic engineering method, and is transferred into streptomyces diastatochromogenes 1628, the streptomyces diastatochromogenes with high yield of toyocamycin overexpresses a streptomyces coelicolor ppGpp synthetase RelA gene, and the overexpression product of the RelA gene can positively regulate and control the biosynthesis of toyocamycin, and is used for the production of toyocamycin. The yield of toyocamycin produced by fermentation of the strain obtained by the invention is at least increased to 1568mg / L, which is 10 times of the yield of the original strain, and a new technical support is provided for increasing the yield of toyocamycin in industrial production.

The invention discloses a gene cluster expression strengthened recombinant streptomyces diastatochromogenes and a construction method thereof. The gene cluster expression strengthened recombinant streptomyces diastatochromogenes excessively expresses a whole toy gene cluster for biosynthesizing toyocamycin and has stronger toyocamycin synthetic capability than streptomyces diastatochromogenes 1628. A construction process comprises the following steps: 1) constructing an expression vector pSET152-toyAM; and 2) integrating the expression vector into chromosome of the streptomyces diastatochromogenes by utilizing a conjugative transferring method to obtain an engineering bacterium. According to the construction method disclosed by the invention, specificity of the vector pSET152-toyAM is integrated into the chromosome of the streptomyces diastatochromogenes 1628 by utilizing the conjugative transferring method to obtain an engineering bacterium with genetic stability. Compared with an original strain, the gene cluster expression strengthened recombinant streptomyces diastatochromogenes has the advantage that the yield of toyocamycin is increased by 4.5 times.

The invention discloses a method for effectively increasing the yield of tetraene macrolide antibiotics, and belongs to the field of microbial technologies. According to the method, by screening joint drug-resistant mutant strains, mutant strains of streptomyces diastatochromogenes D with drug resistance to rifampicin, low-concentration streptomycin and high-concentration streptomycin are obtained, and compared with streptomyces diastatochromogenes D, the capability of the obtained mutant strains for synthesizing tetraene macrolide antibiotics is significantly improved.

The invention discloses a toyG expression-enhanced recombined streptomyces diastatochromogenes and a construction method and uses thereof. The recombined streptomyces diastatochromogenes excessively expresses that the key enzyme of biosynthetic toyocamycin-an adenosine succinic acid synthetase encoding gene toyG has a toyocamycin synthesis capacity higher than streptomyces diastatochromogenes 1628. The construction process is as follows: (1) constructing an expression vector pIB139-toyG; (2) utilizing a conjugational transfer method to integrate the expression vector into a chromosome of streptomyces diastatochromogenes to obtain engineering bacteria. A promoter permE on the vector pIB139 is utilized to open the expression of the toyG gene, and the conjugational transfer method is utilized to integrate the specificity of the vector pIB139-toyG into the chromosome of streptomyces diastatochromogenes 1628 to obtain the genetic-stability engineering bacteria. Compared with parent strains, the enzyme activity of recombinant strain adenosine succinic acid synthetase is increased by at least 1.2 times, and the yield of toyocamycin is increased by at least 27.1 percent.