The invention relates to a genetically-engineered strain streptomyces diastatochromogenes with high production of epsilon-polylysine. The streptomyces diastatochromogenes is obtained by over-expressing three key genes, in different metabolic pathways, of a succinate dehydrogenase gene sdhB, a lysine / ornithine decarboxylase gene dcdA and an asparagine synthetase gene asnO in streptomyces diastatochromogenes TUST separately. According to the genetically-engineered strain streptomyces diastatochromogenes with high production of the epsilon-polylysine, by over-expressing the key genes in the different metabolic pathways, the genetically-engineered recombinant strain is obtained, and an experiment proves that the epsilon-polylysine-producing capability of the streptomyces genetically-engineeredstrain is improved compared with that of the original strain streptomyces diastatochromogenes TUST in the same situation, so that the excellent strain is provided for production of epsilon-polylysine.