Streptomyces diastochromogenes, fermentation product and application thereof

A technology of Streptomyces chromogenes and amylase, applied in the field of microorganisms, can solve problems such as complex pest control, neglect of natural regulation, and environmental impact, and achieve good control effects, environmental friendliness, and low cost

Inactive Publication Date: 2010-03-31
SHANGHAI PESTICIDE RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to long-term dependence on chemical pesticides and neglect of natural regulation, pest control tends to be complicated and difficult
Moreover, many pharmaceuticals have problems such as large dosage, high toxicity, and impact on the environment. They do not meet the requirements of the current society, and new products are urgently needed to replace them.

Method used

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  • Streptomyces diastochromogenes, fermentation product and application thereof
  • Streptomyces diastochromogenes, fermentation product and application thereof
  • Streptomyces diastochromogenes, fermentation product and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 , fermentation of strain 11339

[0050] Seed medium: glucose 2%, starch 5%, peanut cake powder 2%, soybean cake powder 1%, peptone 0.1%, yeast extract 0.4%, sodium chloride 0.2%, magnesium sulfate 0.5%, ammonium nitrate 0.2%, phosphoric acid Dipotassium hydrogen 0.05%, pH 7.2.

[0051] Fermentation medium: same as seed medium.

[0052] The seed medium was prepared according to the above formula, and then the bacterial strain 11399 growing on the Gaussian slant was inoculated in the prepared medium, and cultured at 28°C for 36-40 hours to obtain the seed liquid.

[0053] The seed liquid obtained above is taken, inoculated into the fermentation culture liquid composed of the above-mentioned culture medium at a ratio of 5-20%, and cultured with aeration and stirring at 28° C. for 90-96 hours to obtain a fermentation liquid.

Embodiment 2

[0054] Example 2 , Extraction of active ingredients

[0055] Get 10 kilograms of fermented culture liquid that embodiment 1 obtains, filter, obtain 8 kilograms of supernatants, process with membrane filtration after acidifying to PH4~5 with hydrochloric acid, obtain 10 kilograms of feed liquids, then use 1 kilogram of macroporous adsorption resin 1300 type Carry out adsorption, carry out eluting with 3 kilograms of ethanol water (alcohol: water=1: 1) (volume ratio), eluate is concentrated under reduced pressure, obtains 45 gram brown solids I, then with 400ml methanol water (methanol: water= 90:10) (volume ratio) was dissolved and left overnight in the refrigerator to obtain 10 g of white solid II.

Embodiment 3

[0056] Example 3 , The structure of the active ingredient is determined

[0057] 3.1. Determination of UV absorption spectrum

[0058] 0.5 mg of compound II obtained in Example 2 was dissolved in 50 ml of methanol, 3 ml was added to a colorimetric tank, and methanol was used as a blank control, and measured on an ultraviolet full-wavelength scanner (UV-2501PC, Shimadzu Corporation, Japan), Measure wavelength from 400nm to 200nm, obtain the ultraviolet absorption spectrum of this compound ( figure 2 ).

[0059] Depend on figure 2 It can be seen from the ultraviolet absorption spectrum that the compound II has absorption at the end.

[0060] 3.2. Determination of H NMR spectrum

[0061] Take 10 mg of compound II obtained in Example 2, dissolve it in 0.5 ml of deuterated chloroform, take tetramethylsilane TMS as the reference substance for measuring the chemical shift, and scan it on a VARIAN 500 MHz nuclear magnetic resonance instrument (VARIAN COMPANY) to obtain the com...

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PUM

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Abstract

The invention discloses a Streptomyces diastochromogenes, a fermentation product 5'-epi-SPA-6952A and an application thereof. The Streptomyces diastochromogenes of the invention has the preservation number of CGMCCNo.2281, the fermentation product thereof has favourable insecticidal activity, and the active ingredient with insecticidal activity in the fermentation product is 5'-epi-SPA-6952A. Thefermentation liquor and the active ingredient 5'-epi-SPA-6952A of the Streptomyces diastochromogenes of the invention both have favourable insecticidal activity and low production cost, is friendly to environment and has favourable application prospect. In addition, the compound 5'-epi-SPA-6952A is a new compound, thus having wide development and application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to an amylase Streptomycesdiastochromogenes SSPRC-11339, its fermentation product and its application. Background technique [0002] Chemical pesticide control of pests has played a very important role in the development of agricultural production. However, due to long-term dependence on chemical pesticides and neglect of natural regulation, pest control tends to be complicated and difficult. Moreover, many pharmaceuticals have problems such as large dosage, high toxicity, and impact on the environment. They do not meet the requirements of the current society, and new products are urgently needed to replace them. [0003] In order to effectively control insect pests, the research and development of biopesticides (including agricultural antibiotics) has attracted extensive attention worldwide. In addition, with the acceleration of my country's accession to WTO, i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P17/18A01N63/02A01N43/90A01P7/00C12R1/525
Inventor 陶黎明王金来旷文丰郭磊徐文平顾学斌杨星王磊张育雷薛章荣陆迪生倪玮玮
Owner SHANGHAI PESTICIDE RES INST
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