Application of toyocamycin to controlling tomato gray mold

A technology for tomato botrytis cinerea and toyomycin, which is applied in the field of microorganisms and can solve the problems of unsafe microbial source pesticide types and the like

Inactive Publication Date: 2011-02-02
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of actinomycete-amylase chromogenous Streptomyces D isolated and screened out from Hangzhou soil for the deficiency that chemical pesticide is unsafe and microbial source pesticide

Method used

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  • Application of toyocamycin to controlling tomato gray mold
  • Application of toyocamycin to controlling tomato gray mold
  • Application of toyocamycin to controlling tomato gray mold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: (Isolation and Screening of Amylase Streptomyces Chromogenes D)

[0022] The present invention obtained the amylase chromogenic Streptomyces chromogen from the soil sample collected in Tianmu Mountain, Lin'an, Zhejiang Province in August 2006, through the steps of separation, cultivation, fermentation, activity determination and screening of effective strains for inhibiting plant fungal disease pathogens. diastatochromogenes) D, preservation.

[0023] Determination of antibacterial activity of amylase Streptomyces diastatochromogenes D: Take 1mL of its fermentation supernatant in a sterile petri dish, mix it with 9mL of PDA medium cooled to 50°C, and mix it evenly with 9mL of PDA medium cooled to 50°C. Place a 4mm diameter cake of cucumber fusarium wilt, tomato botrytis cinerea or tomato early blight respectively on the base plane. Cultivate at ℃ for 36 hours, use sterile water as the control, and repeat 3 times; use the cross method to measure the colony...

Embodiment 2

[0026] Embodiment 2: (preparation method of amylase Streptomyces chromogenes D fermentation metabolite)

[0027] Follow these steps:

[0028] (1) Bacteria test tube slant activation culture: the slant medium is Gaoshi No. 1 medium, and its components and content are: K 2 HPO 4 0.5g, KNO 3 1g, MgSO 4 0.5g, 20g soluble starch, 0.5g NaCl, FeSO 4 0.01g, 20g of agar, add water to 1L after dissolving; pick a little amylase spores of Streptomyces chromogenicum D with an inoculation loop under aseptic conditions, and put them into the sterilized Gaoshi No. 1 medium test tube (15×150mm ), placed in an incubator at 28°C±1°C, activated for 96 hours, until the slant of the test tube is covered with spores;

[0029] (2) Fermentation culture: The fermentation medium contains 21.5g of yellow corn flour, 10g of soluble starch, 5g of bran, and CaCO per 1000mL. 3 2.5g, NH 4 NO 3 1.5g, FeSO 4 0.5g, KH 2 PO 4 1.5g, the balance is water; 40mL fermentation broth per 300mL triangular bott...

Embodiment 3

[0033] Embodiment 3: (use amylase Streptomyces chromogenes D fermentation metabolite to prepare wettable powder)

[0034] in:

[0035] White carbon black: (produced by Shanghai Fuqi Chemical Co., Ltd.)

[0036] Nongdai 0203-B: (produced by Xingtai Lanxing Auxiliary Factory)

[0037] Sodium lignosulfonate: (produced by Shanghai Weicheng Chemical Co., Ltd.)

[0038] Light calcium carbonate: (produced by Shanghai Daewoo Biochemical Co., Ltd.)

[0039] The product contains 1.5% active compound Toyocamycin, adding 2% white carbon black as an adsorbent, adding 1% Nongru 0203-B and 3% sodium lignosulfonate auxiliary materials, and finally making up to 100% with light calcium carbonate , after mixing, wettable powder (hereinafter referred to as "product A") was made by jet milling.

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Abstract

The invention discloses a preparation method of a microbial pesticide for controlling vegetable fungus diseases, belonging to the technical field of microorganisms. The preparation method comprises the steps of: activating and culturing strains of streptomyces diastatochromogenes D obtained by the separation and screening of soil samples acquired from Tianmu Mountain in Lin'an, Hangzhou Zhejiang;fermenting and culturing; extracting fermented materials; and preparing the purified active compounds by matching with agricultural pesticide auxiliary materials, and the like into various formationsof agricultural pesticides. The microbial pesticide can be used for controlling vegetable fungus diseases of cucumber fusarium wilt, tomato gray mold or tomato early blight, and the like, and has control effect which is equal or slightly superior to the traditional chemical pesticide, and is safe.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to the cultivation of biocontrol actinomycete-amylase Streptomyces diastatochromogenes D and the separation and application of metabolites. Background technique [0002] Actinomycetes are closely related to humans. According to statistics, 70% of the discovered antibiotics are produced by actinomycetes. Soil is an important place for actinomycetes to inhabit. Obtaining actinomycetes with high antibacterial activity from soil to produce antibiotics is an indispensable basic work for the development of new medicines and new pesticides worldwide. [0003] At present, the control of plant diseases mainly adopts the method of chemical pesticide control. Although chemical pesticides have ideal control effects on many diseases, long-term use will induce the resistance of bacteria. In addition, the frequent use of pesticides not only increases the production cost of agricultural product...

Claims

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Application Information

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IPC IPC(8): A01P3/00A01N43/90C12P19/40C12R1/525
Inventor 俞晓平申屠旭萍董胜张郝培应边亚琳陈春
Owner CHINA JILIANG UNIV
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