Biocontrol actinomyces-streptomyces diastatochromogenes D

A technology of Streptomyces chromogenes and amylase, applied in the field of microorganisms, can solve problems such as the types of unsafe microbial sources of pesticides

Inactive Publication Date: 2008-07-23
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of actinomycete-amylase chromogenous Streptomyces D isolated and screened out from Hangzhou soil for the deficiency that chemical pesticide is unsafe and microbial source pesticide kind is less; Another purpose of the present invention is Provides a microbial pesticide for preventing and treating some plant fungal diseases by using the strain to extract its metabolites through fermentation and a preparation method thereof

Method used

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  • Biocontrol actinomyces-streptomyces diastatochromogenes D
  • Biocontrol actinomyces-streptomyces diastatochromogenes D

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Embodiment 1: (Isolation and Screening of Amylase Streptomyces Chromogenes D)

[0022] The present invention obtained the amylase chromogenic Streptomyces chromogen from the soil sample collected in Tianmu Mountain, Lin'an, Zhejiang Province in August 2006, through the steps of separation, cultivation, fermentation, activity determination and screening of effective strains for inhibiting plant fungal disease pathogens. diastatochromogenes) D, preservation.

[0023] Determination of antibacterial activity of amylase Streptomyces diastatochromogenes D: Take 1mL of its fermentation supernatant in a sterile petri dish, mix it with 9mL of PDA medium cooled to 50°C, and mix it evenly with 9mL of PDA medium cooled to 50°C. Place a 4mm-diameter cake of cucumber fusarium wilt or tomato gray mold on the base plane, connect the cake to the center of the petri dish (the diameter of the petri dish is 9cm), and place it in an incubator for 36 hours at 28°C. Sterile water treatment w...

Embodiment 2

[0026] Embodiment 2: (preparation method of amylase Streptomyces chromogenes D fermentation metabolite)

[0027] Follow these steps:

[0028] (1) Bacteria test tube slant activation culture: the slant medium is Gaoshi No. 1 medium, and its components and content are: K 2 HPO 4 0.5g, KNO 3 1g, MgSO 4 0.5g, 20g soluble starch, 0.5g NaCl, FeSO 4 0.01g, 20g of agar, add water to 1L after dissolving; pick a little amylase spores of Streptomyces chromogenicum D with an inoculation loop under aseptic conditions, and put them into the sterilized Gaoshi No. 1 medium test tube (15×150mm ), placed in an incubator at 28°C±1°C, activated for 96 hours, until the slant of the test tube is covered with spores;

[0029] (2) Fermentation culture: the fermentation medium contains 20g of soybean cake powder, 15g of corn flour, 10g of bran, and NH 4 NO 3 3g, (NH 4 ) 2 8O 4 5g, CaCO 3 5g, MgSO 4 1g, the balance is water; each 300mL triangular bottle is filled with 40mL fermentation brot...

Embodiment 3

[0032] Embodiment 3: (use amylase Streptomyces chromogenes D fermentation metabolite to prepare wettable powder)

[0033] in:

[0034] White carbon black: (produced by Shanghai Fuqi Chemical Co., Ltd.)

[0035] Nongdai 0203-B: (produced by Xingtai Lanxing Auxiliary Factory)

[0036] Sodium lignosulfonate: (produced by Shanghai Weicheng Chemical Co., Ltd.)

[0037] Light calcium carbonate: (produced by Shanghai Daewoo Biochemical Co., Ltd.)

[0038]The product contains 1.5% active compound [2,4-Dioxo-1-(3,4,5-trihydroxy-6-hydroxymethyl-tetrahydro-pyran-2-yl)-1,2,3,4-tetrahydro-pyrimidin-5 -yl]-methylamino-acetic acid, add 2% of white carbon black as an adsorbent, and add 1% of agricultural milk 0203-B and 3% of sodium lignosulfonate auxiliary materials, and finally make up to 100 with light calcium carbonate %, after mixing, the wettable powder (hereinafter referred to as "product A") was made by jet milling.

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Abstract

The invention discloses a preparation method of microbial pesticide for controlling vegetable fungal diseases, belonging to the microbial technical field, which utilizes a streptomyces diastatochromogenes D isolated and screened from a soil sample to prepare various formulation pesticides with the steps of the activation cultivation of strain, fermentation cultivation, leavening extraction and distribution of pesticide auxiliary material to the purified active composite, wherein the soil sample is acquired from Lin' an Tianmu mountain in Hangzhou, Zhejiang. The preparation method of microbial pesticide for controlling vegetable fungal diseases is safe and can be used for controlling cucumber fusarium wilt, tomato gray mold, and other vegetable fungal diseases; and the controlling effect is a little better than normal chemical agents.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the cultivation of a biocontrol actinomycete-amylase streptomyces chromogenicum D and the separation and application of its metabolites. Background technique [0002] Actinomycetes are closely related to humans. According to statistics, 61.7% of the discovered antibiotics are produced by actinomycetes. Commonly used antibiotics except penicillins and cephalosporins, the vast majority are the products of actinomycetes. Soil is an important place for actinomycetes to inhabit. Obtaining actinomycetes with high antibacterial activity from soil to produce antibiotics is an indispensable basic work for the development of new medicines and new pesticides worldwide. [0003] At present, the prevention and control of plant diseases mainly uses chemical pesticides to kill pathogenic bacteria, but the toxic side effects and residues of chemical pesticides on humans and animals are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/465
Inventor 申屠旭萍俞晓平董胜张王为民
Owner CHINA JILIANG UNIV
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