Application of mixed formula preparation to control on nilaparvata lugens stal
A mixed formula, brown planthopper technology, applied in the field of microorganisms, can solve problems such as increased mortality and hindered growth and reproduction of insects
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Embodiment 1
[0014] Embodiment 1: (the preparation method of amylase Streptomyces chromogenes D metabolite)
[0015] Follow these steps:
[0016] (1) Activation culture of strains: move the separated and preserved amylase chromogenic Streptomyces strain D to the slant of Gaoshi No. 1 in the test tube, and cultivate it until the slant of the test tube is covered with spores under the condition of 28°C±1°C;
[0017] (2) Fermentation culture: the fermentation medium contains 20g of soybean cake powder, 15g of corn flour, 10g of bran, and NH 4 NO 3 3g, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 1g, sterilized, cooled to 40°C, inoculated under aseptic conditions with spores of a metalloplatinase amylase Streptomyces chromogenicum D into the fermentation medium, cultured at 28°C±1°C for 5 days;
[0018] (3) Extraction of fermented product: After the fermentation is completed, the fermented liquid is collected by centrifugation, added an equal volume of ethyl acetate for extraction, the lower la...
Embodiment 2
[0020] Embodiment 2: (preparation of amylase Streptomyces chromogenes D metabolite and imidacloprid mixed formula)
[0021] Imidacloprid water-dispersible granules (the active ingredient mass percentage is 70%) are produced by Bayer, and imidacloprid water-dispersible granules and amylase chromogenic Streptomyces D metabolites are dissolved with tap water respectively, mix, and be made into final concentration and be 0.2g / Mixed formula of L-amylase Streptomyces chromogenes D metabolite and 0.01g / L imidacloprid.
Embodiment 3
[0022] Embodiment 3: (Application of amylase Streptomyces chromogenicum D metabolite and imidacloprid mixed formula in the control of brown planthopper nymphs)
[0023] Follow these steps:
[0024] (1) TN1 paddy rice at the tillering and jointing stage, after removing part of the roots and base leaf sheaths, wash and dry naturally;
[0025] (2) With clear water as the control, the final concentration of spraying is 0.2g / L amylase Streptomyces chromogenes D metabolite and 0.01g / L imidacloprid mixed agent. It is required that the spray droplets of the agent are evenly distributed, the coverage rate is high, and the dosage is appropriate. It is advisable to wet the surface of the target object without loss, and let it dry naturally;
[0026] (3) Put the rice seedlings processed in step (2) into a large test tube injected with 20ml rice culture solution in advance, insert 3 rice seedlings into 3 instar brown planthopper nymphs in each tube, and use the rice seedlings after sprayi...
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