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Application of mixed formula preparation to control on nilaparvata lugens stal

A mixed formula, brown planthopper technology, applied in the field of microorganisms, can solve problems such as increased mortality and hindered growth and reproduction of insects

Active Publication Date: 2014-08-06
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The lack of symbiotic bacteria will directly cause the growth and reproduction of insects to be hindered, and the mortality rate will increase.

Method used

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  • Application of mixed formula preparation to control on nilaparvata lugens stal
  • Application of mixed formula preparation to control on nilaparvata lugens stal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: (the preparation method of amylase Streptomyces chromogenes D metabolite)

[0015] Follow these steps:

[0016] (1) Activation culture of strains: move the separated and preserved amylase chromogenic Streptomyces strain D to the slant of Gaoshi No. 1 in the test tube, and cultivate it until the slant of the test tube is covered with spores under the condition of 28°C±1°C;

[0017] (2) Fermentation culture: the fermentation medium contains 20g of soybean cake powder, 15g of corn flour, 10g of bran, and NH 4 NO 3 3g, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 1g, sterilized, cooled to 40°C, inoculated under aseptic conditions with spores of a metalloplatinase amylase Streptomyces chromogenicum D into the fermentation medium, cultured at 28°C±1°C for 5 days;

[0018] (3) Extraction of fermented product: After the fermentation is completed, the fermented liquid is collected by centrifugation, added an equal volume of ethyl acetate for extraction, the lower la...

Embodiment 2

[0020] Embodiment 2: (preparation of amylase Streptomyces chromogenes D metabolite and imidacloprid mixed formula)

[0021] Imidacloprid water-dispersible granules (the active ingredient mass percentage is 70%) are produced by Bayer, and imidacloprid water-dispersible granules and amylase chromogenic Streptomyces D metabolites are dissolved with tap water respectively, mix, and be made into final concentration and be 0.2g / Mixed formula of L-amylase Streptomyces chromogenes D metabolite and 0.01g / L imidacloprid.

Embodiment 3

[0022] Embodiment 3: (Application of amylase Streptomyces chromogenicum D metabolite and imidacloprid mixed formula in the control of brown planthopper nymphs)

[0023] Follow these steps:

[0024] (1) TN1 paddy rice at the tillering and jointing stage, after removing part of the roots and base leaf sheaths, wash and dry naturally;

[0025] (2) With clear water as the control, the final concentration of spraying is 0.2g / L amylase Streptomyces chromogenes D metabolite and 0.01g / L imidacloprid mixed agent. It is required that the spray droplets of the agent are evenly distributed, the coverage rate is high, and the dosage is appropriate. It is advisable to wet the surface of the target object without loss, and let it dry naturally;

[0026] (3) Put the rice seedlings processed in step (2) into a large test tube injected with 20ml rice culture solution in advance, insert 3 rice seedlings into 3 instar brown planthopper nymphs in each tube, and use the rice seedlings after sprayi...

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Abstract

The invention relates to the technical field of microbiology, particularly to an application of a mixed formula preparation to control on nilaparvata lugens stal. Streptomyces diastatochromogenes is named streptomyces diastatochromogenes D. The strain is preserved in the CGMCC (China General Microbiological Culture Collection Center) of CCCCM(China Committee for Culture Collection of Microorganisms)on May 15th, 2007, and the preservation registration number is CGMCC No.2060. Metabolic products having an inhibition effect on nilaparvata lugens stal yeast-likesymbiotic bacteria can be obtained throughfermentation and extraction of the streptomyces diastatochromogenes D.The application is most significantly characterized in that the metabolic products of the streptomyces diastatochromogenes D and imidacloprid are mixed in certainproportion to prepare a new formula, the usage amount of a pesticide can be obviously reduced when the mixed formula is used for controlling the nilaparvata lugens stal, the control effect on the nilaparvata lugens stal can be effectively improved, and the development of the drug resistance of the nilaparvata lugens stal can be delayed.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a mixed formulation of amylase Streptomyces chromogenes D metabolites and imidacloprid. Background technique [0002] brown planthopper It is one of the important pests of rice. It mainly damages rice plants by sucking phloem juice and spreading rice viruses. When the pests occur, a large area of ​​rice plants will die and fall, resulting in reduced grain production, or even no harvest. In terms of production, chemical pesticides are the main means of controlling brown planthoppers. However, due to the large and frequent use of chemical insecticides, the brown planthopper has developed resistance to many chemical insecticides, resulting in a significant decline in the control effect. Therefore, it is imminent to study and apply new means to reduce the occurrence and damage of rice brown planthopper. [0003] The symbiosis between insects and microorganisms is a ubiqui...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P7/04
Inventor 申屠旭萍俞晓平李丹婷
Owner CHINA JILIANG UNIV
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